A versatile and open source design for one and two photon light sheet microscopy
Abstract Two-photon light sheet microscopy offers great potential for a range of biological applications. Still, its practical implementation is impeded by the high cost of laser sources, the complexity of construction, and the challenges associated with adapting to existing microscope setups. Here,...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-03107-3 |
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| author | Antoine Hubert Thomas Panier Geoffrey Migault Hugo Trentesaux Benoît Beaudou Georges Debrégeas Volker Bormuth |
| author_facet | Antoine Hubert Thomas Panier Geoffrey Migault Hugo Trentesaux Benoît Beaudou Georges Debrégeas Volker Bormuth |
| author_sort | Antoine Hubert |
| collection | DOAJ |
| description | Abstract Two-photon light sheet microscopy offers great potential for a range of biological applications. Still, its practical implementation is impeded by the high cost of laser sources, the complexity of construction, and the challenges associated with adapting to existing microscope setups. Here, we release an open-source design that addresses these limitations by providing detailed building instructions for transforming a brightfield microscope into a versatile one- and two-photon light sheet system. Our design incorporates a specially designed broadband hollow core fiber, enabling the simultaneous utilization of visible laser alongside an expansive pulsed laser source from another setup. This integration allows for uncompromised image resolution and speed. Furthermore, the design reduces the complexity of construction, alignment, and overall cost, thereby significantly enhancing the accessibility of this technology ( https://github.com/LJPZebra/OLU ). |
| format | Article |
| id | doaj-art-752ef57a2e8449dba545fc1cfb43bbdc |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-752ef57a2e8449dba545fc1cfb43bbdc2025-08-20T03:45:32ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-03107-3A versatile and open source design for one and two photon light sheet microscopyAntoine Hubert0Thomas Panier1Geoffrey Migault2Hugo Trentesaux3Benoît Beaudou4Georges Debrégeas5Volker Bormuth6Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP)Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP)Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP)Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP)GLOphotonicsSorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP)Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP)Abstract Two-photon light sheet microscopy offers great potential for a range of biological applications. Still, its practical implementation is impeded by the high cost of laser sources, the complexity of construction, and the challenges associated with adapting to existing microscope setups. Here, we release an open-source design that addresses these limitations by providing detailed building instructions for transforming a brightfield microscope into a versatile one- and two-photon light sheet system. Our design incorporates a specially designed broadband hollow core fiber, enabling the simultaneous utilization of visible laser alongside an expansive pulsed laser source from another setup. This integration allows for uncompromised image resolution and speed. Furthermore, the design reduces the complexity of construction, alignment, and overall cost, thereby significantly enhancing the accessibility of this technology ( https://github.com/LJPZebra/OLU ).https://doi.org/10.1038/s41598-025-03107-3Light-sheet microscopyTwo-photon microscopyHollow core fiberNegative curvature fiberOpen source |
| spellingShingle | Antoine Hubert Thomas Panier Geoffrey Migault Hugo Trentesaux Benoît Beaudou Georges Debrégeas Volker Bormuth A versatile and open source design for one and two photon light sheet microscopy Scientific Reports Light-sheet microscopy Two-photon microscopy Hollow core fiber Negative curvature fiber Open source |
| title | A versatile and open source design for one and two photon light sheet microscopy |
| title_full | A versatile and open source design for one and two photon light sheet microscopy |
| title_fullStr | A versatile and open source design for one and two photon light sheet microscopy |
| title_full_unstemmed | A versatile and open source design for one and two photon light sheet microscopy |
| title_short | A versatile and open source design for one and two photon light sheet microscopy |
| title_sort | versatile and open source design for one and two photon light sheet microscopy |
| topic | Light-sheet microscopy Two-photon microscopy Hollow core fiber Negative curvature fiber Open source |
| url | https://doi.org/10.1038/s41598-025-03107-3 |
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