In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide
Potato is a major crop whose yield and quality are impacted by the pathogenic fungus Rhizoctonia solani. Here, a control approach based on the use of endophytic bacillus spores and bacterial consortia was assessed as an innovative solution to improve tuber productivity and health. Endophytic potato...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964424000926 |
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| author | Imen Ben Slimene Debez Amani Ben Alaya Ines Karkouch Bilel Khiari Pedro Garcia-Caparros Nouf M. Alyami Ahmed Debez Belhassen Tarhouni Naceur Djébali |
| author_facet | Imen Ben Slimene Debez Amani Ben Alaya Ines Karkouch Bilel Khiari Pedro Garcia-Caparros Nouf M. Alyami Ahmed Debez Belhassen Tarhouni Naceur Djébali |
| author_sort | Imen Ben Slimene Debez |
| collection | DOAJ |
| description | Potato is a major crop whose yield and quality are impacted by the pathogenic fungus Rhizoctonia solani. Here, a control approach based on the use of endophytic bacillus spores and bacterial consortia was assessed as an innovative solution to improve tuber productivity and health. Endophytic potato bacteria extracted from several organs (leaves, stems and roots) evaluated for their antagonistic activity against R. solani by the double culture technique and their ability to rot potato slices. Additionally, the ability of Bacillus strains to generate hydrolytic enzymes and produce lipopeptides (using ESI-MS/MS mass spectrometry) was assessed. Then, the effectiveness of selected bacterial strains, either individually or in consortium (after carrying out compatibility tests) in the suppression of R. solani was evaluated in the greenhouse. Six endophytes not affecting potato tubers and having an antagonistic effect against R. solani were identified: B. halotolerans SpS5, B. velezensis KnL15, B. aryabhattai FaR1, B. amyloliquefaciens LiR9, B. haynesii ReR10 which produce several types and combinations of lipopeptides belonging to different families (iturins, fengycins and surfactins) as well as hydrolytic enzymes (chitinase, protease and cellulase). The vegetative and sporulating strain SpS5, as well as the 5 other Bacillus and their Sps5-based consortia, were found to be very effective in increasing the yield of healthy, high-quality tubers and reducing disease severity. Based on the results mentioned above, a microbial fungicide from the spores of (i) B. halotolerans SpS5 for which we sequenced the whole genome or (ii) bacterial consortia could be developed to combat black scurf potato and other fungal infections. |
| format | Article |
| id | doaj-art-74446bfebb4f447fa2dca1ff1e5be48b |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-74446bfebb4f447fa2dca1ff1e5be48b2024-11-21T06:03:12ZengElsevierBiological Control1049-96442024-06-01193105527In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicideImen Ben Slimene Debez0Amani Ben Alaya1Ines Karkouch2Bilel Khiari3Pedro Garcia-Caparros4Nouf M. Alyami5Ahmed Debez6Belhassen Tarhouni7Naceur Djébali8Laboratory of Bioactive Substances, Centre of Biotechnology of BorjCedria (CBBC), BP 901 Hammam-Lif 2050, Tunisia; Corresponding author at: Laboratory of Bioactive Substances, Centre of Biotechnology of BorjCedria (CBBC), BP 901 Hammam-Lif 2050, Tunisia.Laboratory of Bioactive Substances, Centre of Biotechnology of BorjCedria (CBBC), BP 901 Hammam-Lif 2050, TunisiaLaboratory of Bioactive Substances, Centre of Biotechnology of BorjCedria (CBBC), BP 901 Hammam-Lif 2050, TunisiaLaboratory of Bioactive Substances, Centre of Biotechnology of BorjCedria (CBBC), BP 901 Hammam-Lif 2050, TunisiaAgronomy Department of Superior School Engineering, University of Almería, Almería, SpainDepartment of Zoology, College of Science, King Saud University, PO Box -2455, Riyadh 11451, Saudi ArabiaLaboratory of Extremophile Plants (LPE), Centre of Biotechnology of Borj Cedria, BP 901 Hammam-Lif 2050, TunisiaCentre Technique de la Pomme de Terre et de l'Artichaut, Route Jedaida, Essaida 2031, Manouba, TunisiaLaboratory of Bioactive Substances, Centre of Biotechnology of BorjCedria (CBBC), BP 901 Hammam-Lif 2050, TunisiaPotato is a major crop whose yield and quality are impacted by the pathogenic fungus Rhizoctonia solani. Here, a control approach based on the use of endophytic bacillus spores and bacterial consortia was assessed as an innovative solution to improve tuber productivity and health. Endophytic potato bacteria extracted from several organs (leaves, stems and roots) evaluated for their antagonistic activity against R. solani by the double culture technique and their ability to rot potato slices. Additionally, the ability of Bacillus strains to generate hydrolytic enzymes and produce lipopeptides (using ESI-MS/MS mass spectrometry) was assessed. Then, the effectiveness of selected bacterial strains, either individually or in consortium (after carrying out compatibility tests) in the suppression of R. solani was evaluated in the greenhouse. Six endophytes not affecting potato tubers and having an antagonistic effect against R. solani were identified: B. halotolerans SpS5, B. velezensis KnL15, B. aryabhattai FaR1, B. amyloliquefaciens LiR9, B. haynesii ReR10 which produce several types and combinations of lipopeptides belonging to different families (iturins, fengycins and surfactins) as well as hydrolytic enzymes (chitinase, protease and cellulase). The vegetative and sporulating strain SpS5, as well as the 5 other Bacillus and their Sps5-based consortia, were found to be very effective in increasing the yield of healthy, high-quality tubers and reducing disease severity. Based on the results mentioned above, a microbial fungicide from the spores of (i) B. halotolerans SpS5 for which we sequenced the whole genome or (ii) bacterial consortia could be developed to combat black scurf potato and other fungal infections.http://www.sciencedirect.com/science/article/pii/S1049964424000926Bacillus sporesLipopeptidesConsortiaPotato black scurfBiocontrolTuber quality |
| spellingShingle | Imen Ben Slimene Debez Amani Ben Alaya Ines Karkouch Bilel Khiari Pedro Garcia-Caparros Nouf M. Alyami Ahmed Debez Belhassen Tarhouni Naceur Djébali In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide Biological Control Bacillus spores Lipopeptides Consortia Potato black scurf Biocontrol Tuber quality |
| title | In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide |
| title_full | In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide |
| title_fullStr | In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide |
| title_full_unstemmed | In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide |
| title_short | In vitro and in vivo antifungal efficacy of individual and consortium Bacillus strains in controlling potato black scurf and possible development of spore-based fungicide |
| title_sort | in vitro and in vivo antifungal efficacy of individual and consortium bacillus strains in controlling potato black scurf and possible development of spore based fungicide |
| topic | Bacillus spores Lipopeptides Consortia Potato black scurf Biocontrol Tuber quality |
| url | http://www.sciencedirect.com/science/article/pii/S1049964424000926 |
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