High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemic
Background and Objectives: Klebsiella pneumoniae is a common pathogen associated with healthcare-related infections. It is particularly notable for its ability to develop resistance to multiple antibiotics, making treatment challenging. During the COVID-19 pandemic, increased antibiotic use to mana...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4892 |
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| author | Golnar Rahimzadeh Shaghayegh Rezai Ghazal Abbasi Saman Soleimanpour Reza Valadan Laleh Vahedi Somayeh Sheidaei Faezeh Sadat Movahedi Raha Rezai Mohammad Sadegh Rezai |
| author_facet | Golnar Rahimzadeh Shaghayegh Rezai Ghazal Abbasi Saman Soleimanpour Reza Valadan Laleh Vahedi Somayeh Sheidaei Faezeh Sadat Movahedi Raha Rezai Mohammad Sadegh Rezai |
| author_sort | Golnar Rahimzadeh |
| collection | DOAJ |
| description |
Background and Objectives: Klebsiella pneumoniae is a common pathogen associated with healthcare-related infections. It is particularly notable for its ability to develop resistance to multiple antibiotics, making treatment challenging. During the COVID-19 pandemic, increased antibiotic use to manage critically ill patients was contributed to the rise of multidrug-resistant Klebsiella pneumoniae. This study evaluated the antibiotic resistance patterns of multidrug-resistant, ESBL-producing Klebsiella pneumoniae in northern Iran after the COVID-19 pandemic.
Materials and Methods: This cross-sectional study was conducted between September 2022 and October 2023. Clinical samples were collected from patients with nosocomial infections at hospitals in Sari. This study included 114 multidrug-resistant ESBLs-producing Klebsiella pneumoniae isolates. Antimicrobial susceptibility was assessed using broth macro-dilution, and resistance genes were detected by multiplex PCR.
Results: Gentamicin, ampicillin-sulbactam, co-amoxiclav, and ceftazidime displayed the lowest activity against multidrug-resistant Klebsiella pneumoniae. In contrast, piperacillin-tazobactam showed the highest activity. The prevalence of resistance genes was as follows: blaTEM (99.12%), blaSHV (74.56%), blaCTX (88.60%), blaIMP (64.04%), acrA -B (92.98%), and OqXA -B (67.54%).
Conclusion: This study identified over 50% of antibiotic-resistance genes. Over half of multidrug-resistant Klebsiella pneumoniae isolates showed resistance to antibiotics except piperacillin-tazobactam, which is recommended for treating multidrug-resistant Klebsiella pneumoniae infections.
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| format | Article |
| id | doaj-art-73d3cfd879d84e52abb7c1f8faccf5ae |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-73d3cfd879d84e52abb7c1f8faccf5ae2025-01-06T08:38:48ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-12-0116610.18502/ijm.v16i6.17247High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemicGolnar Rahimzadeh0Shaghayegh Rezai1Ghazal Abbasi2Saman Soleimanpour3Reza Valadan4Laleh Vahedi5Somayeh Sheidaei6Faezeh Sadat Movahedi7Raha Rezai8Mohammad Sadegh Rezai9Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranStudents Research Committee, Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMolecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranDepartment of Pathology, School of Medicine, Mazandaran University of Medical Sciences, Sari, IranDepartment of Pathology, School of Medicine, Mazandaran University of Medical Sciences, Sari, IranPediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, IranPediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, IranPediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran Background and Objectives: Klebsiella pneumoniae is a common pathogen associated with healthcare-related infections. It is particularly notable for its ability to develop resistance to multiple antibiotics, making treatment challenging. During the COVID-19 pandemic, increased antibiotic use to manage critically ill patients was contributed to the rise of multidrug-resistant Klebsiella pneumoniae. This study evaluated the antibiotic resistance patterns of multidrug-resistant, ESBL-producing Klebsiella pneumoniae in northern Iran after the COVID-19 pandemic. Materials and Methods: This cross-sectional study was conducted between September 2022 and October 2023. Clinical samples were collected from patients with nosocomial infections at hospitals in Sari. This study included 114 multidrug-resistant ESBLs-producing Klebsiella pneumoniae isolates. Antimicrobial susceptibility was assessed using broth macro-dilution, and resistance genes were detected by multiplex PCR. Results: Gentamicin, ampicillin-sulbactam, co-amoxiclav, and ceftazidime displayed the lowest activity against multidrug-resistant Klebsiella pneumoniae. In contrast, piperacillin-tazobactam showed the highest activity. The prevalence of resistance genes was as follows: blaTEM (99.12%), blaSHV (74.56%), blaCTX (88.60%), blaIMP (64.04%), acrA -B (92.98%), and OqXA -B (67.54%). Conclusion: This study identified over 50% of antibiotic-resistance genes. Over half of multidrug-resistant Klebsiella pneumoniae isolates showed resistance to antibiotics except piperacillin-tazobactam, which is recommended for treating multidrug-resistant Klebsiella pneumoniae infections. https://ijm.tums.ac.ir/index.php/ijm/article/view/4892Klebsiella pneumoniae;Healthcare associated infections;COVID‑19;Extended spectrum beta lactamase;Multidrug resistant;Multiplex polymerase chain reaction |
| spellingShingle | Golnar Rahimzadeh Shaghayegh Rezai Ghazal Abbasi Saman Soleimanpour Reza Valadan Laleh Vahedi Somayeh Sheidaei Faezeh Sadat Movahedi Raha Rezai Mohammad Sadegh Rezai High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemic Iranian Journal of Microbiology Klebsiella pneumoniae; Healthcare associated infections; COVID‑19; Extended spectrum beta lactamase; Multidrug resistant; Multiplex polymerase chain reaction |
| title | High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemic |
| title_full | High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemic |
| title_fullStr | High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemic |
| title_full_unstemmed | High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemic |
| title_short | High prevalence of antimicrobial resistance genes in multidrug-resistant-ESBLs-producing Klebsiella pneumoniae post-COVID-19 pandemic |
| title_sort | high prevalence of antimicrobial resistance genes in multidrug resistant esbls producing klebsiella pneumoniae post covid 19 pandemic |
| topic | Klebsiella pneumoniae; Healthcare associated infections; COVID‑19; Extended spectrum beta lactamase; Multidrug resistant; Multiplex polymerase chain reaction |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4892 |
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