Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing
In this study, we present a novel 3D perfused skin-on-a-chip model fabricated using micro-precision 3D printing, which offers a streamlined and reproducible approach for incorporating perfusion. Perfused skin models are well-regarded for their advantages, such as improved nutrient supply, enhanced b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-10-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/11/11/1055 |
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| author | Stephen Rhee Chunguang Xia Aditya Chandra Morgan Hamon Geonhui Lee Chen Yang Zaixun Guo Bingjie Sun |
| author_facet | Stephen Rhee Chunguang Xia Aditya Chandra Morgan Hamon Geonhui Lee Chen Yang Zaixun Guo Bingjie Sun |
| author_sort | Stephen Rhee |
| collection | DOAJ |
| description | In this study, we present a novel 3D perfused skin-on-a-chip model fabricated using micro-precision 3D printing, which offers a streamlined and reproducible approach for incorporating perfusion. Perfused skin models are well-regarded for their advantages, such as improved nutrient supply, enhanced barrier function, and prolonged tissue viability. However, current models often require complex setups, such as self-assembled endothelial cells or sacrificial rods, which are prone to variability and time-consuming. Our model uses projection micro-stereolithography 3D printing to create precise microcapillary-like channels using a biocompatible resin, overcoming the drug-absorbing properties of PDMS. A customized chip holder allows for the simultaneous culture of six perfused chips, enabling high-throughput testing. The engineered skin-on-a-chip features distinct dermis and epidermis layers, confirmed via H&E staining and immunostaining. To evaluate drug screening capabilities, inflammation was induced using TNF-α and treated with dexamethasone, with cytokine levels compared to 2D cultures and human skin biopsies. Our 3D model exhibited drug response trends similar to human skin, while showing reduced cytotoxicity over time compared to biopsies. This perfused skin-on-a-chip provides a reliable, physiologically relevant alternative for drug and cosmetics screening, simplifying perfusion setup while preserving key benefits. |
| format | Article |
| id | doaj-art-71214cf52c6c4a78a8fa6af59d5469f8 |
| institution | Kabale University |
| issn | 2306-5354 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-71214cf52c6c4a78a8fa6af59d5469f82024-11-26T17:51:46ZengMDPI AGBioengineering2306-53542024-10-011111105510.3390/bioengineering11111055Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics TestingStephen Rhee0Chunguang Xia1Aditya Chandra2Morgan Hamon3Geonhui Lee4Chen Yang5Zaixun Guo6Bingjie Sun7BMF Biotechnology, Inc., San Diego, CA 92121, USABMF Biotechnology, Inc., San Diego, CA 92121, USABMF Biotechnology, Inc., San Diego, CA 92121, USABMF Biotechnology, Inc., San Diego, CA 92121, USABMF Biotechnology, Inc., San Diego, CA 92121, USABMF Nano Material Technology Co., Ltd., Shenzhen 518100, ChinaBMF Nano Material Technology Co., Ltd., Shenzhen 518100, ChinaBMF Biotechnology, Inc., San Diego, CA 92121, USAIn this study, we present a novel 3D perfused skin-on-a-chip model fabricated using micro-precision 3D printing, which offers a streamlined and reproducible approach for incorporating perfusion. Perfused skin models are well-regarded for their advantages, such as improved nutrient supply, enhanced barrier function, and prolonged tissue viability. However, current models often require complex setups, such as self-assembled endothelial cells or sacrificial rods, which are prone to variability and time-consuming. Our model uses projection micro-stereolithography 3D printing to create precise microcapillary-like channels using a biocompatible resin, overcoming the drug-absorbing properties of PDMS. A customized chip holder allows for the simultaneous culture of six perfused chips, enabling high-throughput testing. The engineered skin-on-a-chip features distinct dermis and epidermis layers, confirmed via H&E staining and immunostaining. To evaluate drug screening capabilities, inflammation was induced using TNF-α and treated with dexamethasone, with cytokine levels compared to 2D cultures and human skin biopsies. Our 3D model exhibited drug response trends similar to human skin, while showing reduced cytotoxicity over time compared to biopsies. This perfused skin-on-a-chip provides a reliable, physiologically relevant alternative for drug and cosmetics screening, simplifying perfusion setup while preserving key benefits.https://www.mdpi.com/2306-5354/11/11/1055skinorgan-on-a-chipskin-on-a-chipperfusiondrug screening3D printing |
| spellingShingle | Stephen Rhee Chunguang Xia Aditya Chandra Morgan Hamon Geonhui Lee Chen Yang Zaixun Guo Bingjie Sun Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing Bioengineering skin organ-on-a-chip skin-on-a-chip perfusion drug screening 3D printing |
| title | Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing |
| title_full | Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing |
| title_fullStr | Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing |
| title_full_unstemmed | Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing |
| title_short | Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing |
| title_sort | full thickness perfused skin on a chip with in vivo like drug response for drug and cosmetics testing |
| topic | skin organ-on-a-chip skin-on-a-chip perfusion drug screening 3D printing |
| url | https://www.mdpi.com/2306-5354/11/11/1055 |
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