Agarose hydrogel-mediated electroporation method for retinal tissue cultured at the air-liquid interface

Summary: It is advantageous to culture the ex vivo retina and other tissues at the air-liquid interface to allow for more efficient gas exchange. However, gene delivery to these cultures can be challenging. Electroporation is a fast and robust method of gene delivery, but typically requires submerge...

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Bibliographic Details
Main Authors: Megan L. Stone, Hannah H. Lee, Edward M. Levine
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:iScience
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004224025240
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