Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell Lines
<i>Puya chilensis</i> Mol. is a plant of the Bromeliaceae family, which has been traditionally used for medicinal applications in various digestive disorders. In this study, the phytoconstituents of six extracts of stems and flowers of <i>P. chilensis</i> were evaluated: phen...
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2024-10-01
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| author | Manuel Martínez-Lobos Valentina Silva Joan Villena Carlos Jara-Gutiérrez Waleska E. Vera Quezada Iván Montenegro Alejandro Madrid |
| author_facet | Manuel Martínez-Lobos Valentina Silva Joan Villena Carlos Jara-Gutiérrez Waleska E. Vera Quezada Iván Montenegro Alejandro Madrid |
| author_sort | Manuel Martínez-Lobos |
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| description | <i>Puya chilensis</i> Mol. is a plant of the Bromeliaceae family, which has been traditionally used for medicinal applications in various digestive disorders. In this study, the phytoconstituents of six extracts of stems and flowers of <i>P. chilensis</i> were evaluated: phenols, flavonoids and total anthraquinones, as well as their antioxidant capacity and cytotoxicity in colon cancer cell lines HT-29. The data demonstrate that the ethyl acetate extract of <i>P. chilensis</i> flowers is cytotoxic in HT-29 cell lines (IC<sub>50</sub> = 41.70 µg/mL) without causing toxic effects on healthy colon cells (IC<sub>50</sub> > 100 µg/mL); also, this extract concentrated the highest amount of phenols (4.63 μg GAE/g d.e.), flavonoids (31.5 μg QE/g d.e.) and anthraquinones (12.60 μg EE/g d.e.) among all the extracts tested, which also correlated with its highlighted antioxidant capacity (DPPH∙IC<sub>50</sub> = 4.15 mg/mL and FRAP 26.52 mM TEAC) over the other extracts. About thirty-five compounds were identified in this extract−the fatty acid esters present have been shown to have therapeutic effects on several types of cancer and could explain its antiproliferative activity. |
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| institution | Kabale University |
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| publishDate | 2024-10-01 |
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| spelling | doaj-art-6f89caee04c24d209e34d7ae6f3d65df2024-11-08T14:39:11ZengMDPI AGPlants2223-77472024-10-011321298910.3390/plants13212989Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell LinesManuel Martínez-Lobos0Valentina Silva1Joan Villena2Carlos Jara-Gutiérrez3Waleska E. Vera Quezada4Iván Montenegro5Alejandro Madrid6Laboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2340000, ChileLaboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2340000, ChileCentro Interdisciplinario de Investigación Biomédica e Ingeniería para la Salud (MEDING), Escuela de Medicina, Facultad de Medicina, Universidad de Valparaíso, Valparaíso 2340000, ChileCentro Interdisciplinario de Investigación Biomédica e Ingeniería para la Salud (MEDING), Escuela de Medicina, Escuela de Kinesiología, Universidad de Valparaíso, Valparaíso 2340000, ChileLaboratorio de Química de Metabolitos Bioactivos, Escuela de Química y Farmacia, Facultad de Farmacia, Centro de Investigación Farmacopea Chilena, Universidad de Valparaíso, Valparaíso 2340000, ChileCentro Interdisciplinario de Investigación Biomédica e Ingeniería para la Salud (MEDING), Escuela de Obstetricia, Facultad de Medicina, Universidad de Valparaíso, Valparaíso 2340000, ChileLaboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Ciencias y Geografía, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2340000, Chile<i>Puya chilensis</i> Mol. is a plant of the Bromeliaceae family, which has been traditionally used for medicinal applications in various digestive disorders. In this study, the phytoconstituents of six extracts of stems and flowers of <i>P. chilensis</i> were evaluated: phenols, flavonoids and total anthraquinones, as well as their antioxidant capacity and cytotoxicity in colon cancer cell lines HT-29. The data demonstrate that the ethyl acetate extract of <i>P. chilensis</i> flowers is cytotoxic in HT-29 cell lines (IC<sub>50</sub> = 41.70 µg/mL) without causing toxic effects on healthy colon cells (IC<sub>50</sub> > 100 µg/mL); also, this extract concentrated the highest amount of phenols (4.63 μg GAE/g d.e.), flavonoids (31.5 μg QE/g d.e.) and anthraquinones (12.60 μg EE/g d.e.) among all the extracts tested, which also correlated with its highlighted antioxidant capacity (DPPH∙IC<sub>50</sub> = 4.15 mg/mL and FRAP 26.52 mM TEAC) over the other extracts. About thirty-five compounds were identified in this extract−the fatty acid esters present have been shown to have therapeutic effects on several types of cancer and could explain its antiproliferative activity.https://www.mdpi.com/2223-7747/13/21/2989<i>Puya chilensis</i>antioxidantcytotoxiccolon cancerascorbic acid 2,6 dihexadecanoate |
| spellingShingle | Manuel Martínez-Lobos Valentina Silva Joan Villena Carlos Jara-Gutiérrez Waleska E. Vera Quezada Iván Montenegro Alejandro Madrid Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell Lines Plants <i>Puya chilensis</i> antioxidant cytotoxic colon cancer ascorbic acid 2,6 dihexadecanoate |
| title | Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell Lines |
| title_full | Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell Lines |
| title_fullStr | Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell Lines |
| title_full_unstemmed | Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell Lines |
| title_short | Phytoconstituents, Antioxidant Activity and Cytotoxicity of <i>Puya chilensis</i> Mol. Extracts in Colon Cell Lines |
| title_sort | phytoconstituents antioxidant activity and cytotoxicity of i puya chilensis i mol extracts in colon cell lines |
| topic | <i>Puya chilensis</i> antioxidant cytotoxic colon cancer ascorbic acid 2,6 dihexadecanoate |
| url | https://www.mdpi.com/2223-7747/13/21/2989 |
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