Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls
This study examined the effects of surgical castration and lidocaine-plus-meloxicam treatment on growth, physiology, behaviors, and leukocyte heat shock protein 90 (HSP 90) gene expression in Hanwoo (Korean cattle) bulls. Twenty Hanwoo bulls (body weight 248.8 ± 28.5 kg, age 9.4 ± 1.04 months) were...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Veterinary Science |
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| author | Ingu Cho Seonpil Yoo Da Jin Sol Jung Jaesung Lee Seok-Hyeon Baek Sang Yeob Kim Jinoh Lee Dohyun Kim Hyun Jin Kim Myunggi Baik Myunggi Baik |
| author_facet | Ingu Cho Seonpil Yoo Da Jin Sol Jung Jaesung Lee Seok-Hyeon Baek Sang Yeob Kim Jinoh Lee Dohyun Kim Hyun Jin Kim Myunggi Baik Myunggi Baik |
| author_sort | Ingu Cho |
| collection | DOAJ |
| description | This study examined the effects of surgical castration and lidocaine-plus-meloxicam treatment on growth, physiology, behaviors, and leukocyte heat shock protein 90 (HSP 90) gene expression in Hanwoo (Korean cattle) bulls. Twenty Hanwoo bulls (body weight 248.8 ± 28.5 kg, age 9.4 ± 1.04 months) were assigned to three treatments: surgical castration with lidocaine injection (5 mL in the scrotum) and oral meloxicam administration (1 mg/kg body weight; LM; n = 7); surgical castration with placebo injection (5 mL of 0.9% NaCl) and oral placebo administration (lactose, 1 mg/kg body weight; CAS; n = 7); and shame castration (SHAM; n = 6). Meloxicam and lactose were administered 3 h before castration, and lidocaine and NaCl were injected immediately before castration. Surgical castration was performed with a Newberry knife and a Henderson castration tool. Wight was measured the day before and 14 d after castration, and behavior was observed from 0.5 h to 4.5 h post-castration. Blood was collected at −1 d, 0.5 h, 6 h, 1 d, 3 d, 7 d, and 14 d after castration to measure plasma cortisol, haptoglobin, and leukocyte HSP 90 mRNA. Castration tended to decrease average daily gain (p = 0.06), but the LM treatment did not affect weight gain. Bulls in CAS showed higher cortisol concentration (p < 0.05) at 0.5 and 6 h after castration compared to SHAM, with no difference between CAS and LM. Castration increased haptoglobin concentration at 1 and 3 d after castration (p < 0.05) while LM bulls showed decreased haptoglobin concentration (p < 0.05) than CAS bulls at these times. HSP90 mRNA was increased at 6 h post-castration while LM did not reduce its expression, suggesting HSP90 may serve as an acute stress marker in castrated bulls. Castration increased (p < 0.05) frequencies of drinking, lying, walking, leg lifting, kicking, and stiff gait, while decreasing (p < 0.05) eating frequency. LM alleviated (p < 0.05) drinking, leg lifting, kicking, and stiff gait. Collectively, castration resulted in physiological changes, increased leukocyte HSP90 gene expression, and altered behaviors. These findings suggest that lidocaine-plus-meloxicam treatment partially mitigates pain and inflammation in the castrated bulls. |
| format | Article |
| id | doaj-art-6f5ae5fc770c43889e40257511b6fd8d |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-6f5ae5fc770c43889e40257511b6fd8d2024-12-04T07:27:36ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14658441465844Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bullsIngu Cho0Seonpil Yoo1Da Jin Sol Jung2Jaesung Lee3Seok-Hyeon Baek4Sang Yeob Kim5Jinoh Lee6Dohyun Kim7Hyun Jin Kim8Myunggi Baik9Myunggi Baik10Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaAnimal Welfare Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, CanadaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaInstitutes of Green Bio Science Technology, Seoul National University, Pyeongchang-gun, Gangwon, Republic of KoreaThis study examined the effects of surgical castration and lidocaine-plus-meloxicam treatment on growth, physiology, behaviors, and leukocyte heat shock protein 90 (HSP 90) gene expression in Hanwoo (Korean cattle) bulls. Twenty Hanwoo bulls (body weight 248.8 ± 28.5 kg, age 9.4 ± 1.04 months) were assigned to three treatments: surgical castration with lidocaine injection (5 mL in the scrotum) and oral meloxicam administration (1 mg/kg body weight; LM; n = 7); surgical castration with placebo injection (5 mL of 0.9% NaCl) and oral placebo administration (lactose, 1 mg/kg body weight; CAS; n = 7); and shame castration (SHAM; n = 6). Meloxicam and lactose were administered 3 h before castration, and lidocaine and NaCl were injected immediately before castration. Surgical castration was performed with a Newberry knife and a Henderson castration tool. Wight was measured the day before and 14 d after castration, and behavior was observed from 0.5 h to 4.5 h post-castration. Blood was collected at −1 d, 0.5 h, 6 h, 1 d, 3 d, 7 d, and 14 d after castration to measure plasma cortisol, haptoglobin, and leukocyte HSP 90 mRNA. Castration tended to decrease average daily gain (p = 0.06), but the LM treatment did not affect weight gain. Bulls in CAS showed higher cortisol concentration (p < 0.05) at 0.5 and 6 h after castration compared to SHAM, with no difference between CAS and LM. Castration increased haptoglobin concentration at 1 and 3 d after castration (p < 0.05) while LM bulls showed decreased haptoglobin concentration (p < 0.05) than CAS bulls at these times. HSP90 mRNA was increased at 6 h post-castration while LM did not reduce its expression, suggesting HSP90 may serve as an acute stress marker in castrated bulls. Castration increased (p < 0.05) frequencies of drinking, lying, walking, leg lifting, kicking, and stiff gait, while decreasing (p < 0.05) eating frequency. LM alleviated (p < 0.05) drinking, leg lifting, kicking, and stiff gait. Collectively, castration resulted in physiological changes, increased leukocyte HSP90 gene expression, and altered behaviors. These findings suggest that lidocaine-plus-meloxicam treatment partially mitigates pain and inflammation in the castrated bulls.https://www.frontiersin.org/articles/10.3389/fvets.2024.1465844/fullwelfarestressbullnon-steroidal anti-inflammatory druglocal anesthetic |
| spellingShingle | Ingu Cho Seonpil Yoo Da Jin Sol Jung Jaesung Lee Seok-Hyeon Baek Sang Yeob Kim Jinoh Lee Dohyun Kim Hyun Jin Kim Myunggi Baik Myunggi Baik Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls Frontiers in Veterinary Science welfare stress bull non-steroidal anti-inflammatory drug local anesthetic |
| title | Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls |
| title_full | Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls |
| title_fullStr | Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls |
| title_full_unstemmed | Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls |
| title_short | Effects of lidocaine-plus-meloxicam treatment on behavioral and physiological changes, and leukocyte heat shock protein 90 gene expression after surgical castration in Hanwoo bulls |
| title_sort | effects of lidocaine plus meloxicam treatment on behavioral and physiological changes and leukocyte heat shock protein 90 gene expression after surgical castration in hanwoo bulls |
| topic | welfare stress bull non-steroidal anti-inflammatory drug local anesthetic |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1465844/full |
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