A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved]
Stimulator of interferon genes protein (STING1) is an immune adaptor protein which promotes innate immune defense mechanisms against pathogens. To enhance our understanding of STING1-associated disease, it is essential to make high-performing antibodies accessible to the scientific community. This s...
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F1000 Research Ltd
2025-01-01
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| Online Access: | https://f1000research.com/articles/13-1049/v2 |
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| author | Vera Ruíz Moleón Carl Laflamme Kathleen Southern Riham Ayoubi Charles Alende Maryam Fotouhi |
| author_facet | Vera Ruíz Moleón Carl Laflamme Kathleen Southern Riham Ayoubi Charles Alende Maryam Fotouhi |
| author_sort | Vera Ruíz Moleón |
| collection | DOAJ |
| description | Stimulator of interferon genes protein (STING1) is an immune adaptor protein which promotes innate immune defense mechanisms against pathogens. To enhance our understanding of STING1-associated disease, it is essential to make high-performing antibodies accessible to the scientific community. This study aims to improve reliability of STING1 research as we have characterized sixteen STING1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs. |
| format | Article |
| id | doaj-art-6f1788b339044b428901f11ade3cdf1a |
| institution | Kabale University |
| issn | 2046-1402 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj-art-6f1788b339044b428901f11ade3cdf1a2025-01-14T01:00:02ZengF1000 Research LtdF1000Research2046-14022025-01-0113175918A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved]Vera Ruíz Moleón0https://orcid.org/0000-0003-3728-3158Carl Laflamme1https://orcid.org/0000-0001-5906-025XKathleen Southern2https://orcid.org/0000-0002-4125-3608Riham Ayoubi3Charles Alende4https://orcid.org/0009-0005-4611-6134Maryam Fotouhi5Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaStimulator of interferon genes protein (STING1) is an immune adaptor protein which promotes innate immune defense mechanisms against pathogens. To enhance our understanding of STING1-associated disease, it is essential to make high-performing antibodies accessible to the scientific community. This study aims to improve reliability of STING1 research as we have characterized sixteen STING1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.https://f1000research.com/articles/13-1049/v2Uniprot ID: Q86WV6 STING1 hSTING MPYS MITA stimulator of interferon genes proteineng |
| spellingShingle | Vera Ruíz Moleón Carl Laflamme Kathleen Southern Riham Ayoubi Charles Alende Maryam Fotouhi A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] F1000Research Uniprot ID: Q86WV6 STING1 hSTING MPYS MITA stimulator of interferon genes protein eng |
| title | A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_full | A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_fullStr | A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_full_unstemmed | A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_short | A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_sort | guide to selecting high performing antibodies for sting1 uniprot id q86wv6 for use in western blot immunoprecipitation and immunofluorescence version 2 peer review 2 approved |
| topic | Uniprot ID: Q86WV6 STING1 hSTING MPYS MITA stimulator of interferon genes protein eng |
| url | https://f1000research.com/articles/13-1049/v2 |
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