Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat
Stroke is one of the leading causes of death and disability worldwide. However, information on stroke-related tongue coating microbiome (TCM) is limited, and whether TCM modulation could benefit for stroke prevention and rehabilitation is unknown. Here, TCM from stroke patients (SP) was characterize...
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250177 |
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| author | Zhiqiang Xiong Gang Liu Ling Fang Xiuming Li Yongjun Xia Guangqiang Wang Xin Song Lianzhong Ai |
| author_facet | Zhiqiang Xiong Gang Liu Ling Fang Xiuming Li Yongjun Xia Guangqiang Wang Xin Song Lianzhong Ai |
| author_sort | Zhiqiang Xiong |
| collection | DOAJ |
| description | Stroke is one of the leading causes of death and disability worldwide. However, information on stroke-related tongue coating microbiome (TCM) is limited, and whether TCM modulation could benefit for stroke prevention and rehabilitation is unknown. Here, TCM from stroke patients (SP) was characterized using molecular techniques. The occurrence of stroke resulted in TCM dysbiosis with signif icantly reduced species richness and diversity. The abundance of Prevotella, Leptotrichia, Actinomyces, Alloprevotella, Haemophilus, and TM7_[G-1]were greatly reduced, but common infection Streptococcus and Pseudomonas were remarkably increased. Furthermore, an antioxidative probiotic Lactiplantibacillus plantarum AR113 was used for TCM intervention in stroke rats with cerebral ischemia/reperfusion (I/R). AR113 partly restored I/R induced change of TCM and gut microbiota with significantly improved neurological deficit, relieved histopathologic change, increased activities of antioxidant enzymes, and decreased contents of oxidative stress biomarkers. Moreover, the gene expression of antioxidant-related proteins and apoptosis-related factors heme oxygenase-1 (HO-1), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H:quinone oxidoreductase-1 (NQO-1), and Bcl-2 was significantly increased, but cytochrome C, cleaved caspase-3, and Bax were markedly decreased in the brain by AR113 treatment. The results suggested that AR113 could ameliorate cerebral I/R injury through antioxidation and anti-apoptosis pathways, and AR113 intervention of TCM may have the application potential for stroke prevention and control. |
| format | Article |
| id | doaj-art-6ebe0ee99b1c4fd48c02edf95b010210 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-6ebe0ee99b1c4fd48c02edf95b0102102025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342132214010.26599/FSHW.2022.9250177Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in ratZhiqiang Xiong0Gang Liu1Ling Fang2Xiuming Li3Yongjun Xia4Guangqiang Wang5Xin Song6Lianzhong Ai7Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaDepartment of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaDepartment of Rehabilitation Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaStroke is one of the leading causes of death and disability worldwide. However, information on stroke-related tongue coating microbiome (TCM) is limited, and whether TCM modulation could benefit for stroke prevention and rehabilitation is unknown. Here, TCM from stroke patients (SP) was characterized using molecular techniques. The occurrence of stroke resulted in TCM dysbiosis with signif icantly reduced species richness and diversity. The abundance of Prevotella, Leptotrichia, Actinomyces, Alloprevotella, Haemophilus, and TM7_[G-1]were greatly reduced, but common infection Streptococcus and Pseudomonas were remarkably increased. Furthermore, an antioxidative probiotic Lactiplantibacillus plantarum AR113 was used for TCM intervention in stroke rats with cerebral ischemia/reperfusion (I/R). AR113 partly restored I/R induced change of TCM and gut microbiota with significantly improved neurological deficit, relieved histopathologic change, increased activities of antioxidant enzymes, and decreased contents of oxidative stress biomarkers. Moreover, the gene expression of antioxidant-related proteins and apoptosis-related factors heme oxygenase-1 (HO-1), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H:quinone oxidoreductase-1 (NQO-1), and Bcl-2 was significantly increased, but cytochrome C, cleaved caspase-3, and Bax were markedly decreased in the brain by AR113 treatment. The results suggested that AR113 could ameliorate cerebral I/R injury through antioxidation and anti-apoptosis pathways, and AR113 intervention of TCM may have the application potential for stroke prevention and control.https://www.sciopen.com/article/10.26599/FSHW.2022.9250177strokecerebral ischemia/reperfusiontongue coatinglactiplantibacillus plantarum ar113probiotic intervention |
| spellingShingle | Zhiqiang Xiong Gang Liu Ling Fang Xiuming Li Yongjun Xia Guangqiang Wang Xin Song Lianzhong Ai Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat Food Science and Human Wellness stroke cerebral ischemia/reperfusion tongue coating lactiplantibacillus plantarum ar113 probiotic intervention |
| title | Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat |
| title_full | Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat |
| title_fullStr | Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat |
| title_full_unstemmed | Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat |
| title_short | Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat |
| title_sort | lactiplantibacillus plantarum ar113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia reperfusion injury in rat |
| topic | stroke cerebral ischemia/reperfusion tongue coating lactiplantibacillus plantarum ar113 probiotic intervention |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250177 |
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