Construction of primary chassis cells with efficient protein expression in Thermus thermophilus

Construction of primary chassis cells with efficient protein expression in Thermus thermophilus

Abstract Background Thermus thermophilus HB27 is a promising thermophilic chassis for recombinant thermostable protein production, owing to its high optimal growth temperature, which can simplify downstream processing and reduce contamination risks. However, maximizing its potential requires optimiz...

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Bibliographic Details
Main Authors: Yuqian Liang, Mohamed Motawaa, Xuying Bu, Junwei Wei, Yuan Shao, Yingjun Li
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Microbial Cell Factories
Subjects:
Thermus thermophilus HB27
Chassis cell
Promoter library
Β-galactosidase monitor system
pTT27-Plasmid
Protease activity
Online Access:https://doi.org/10.1186/s12934-025-02785-y
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https://doi.org/10.1186/s12934-025-02785-y

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