Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric Nanoparticles

Nitric oxide (NO) is a multifunctional signaling molecule in plants, playing key roles in germination, microbial symbiosis, and nodule formation. However, its instability requires innovative approaches, such as using nanoencapsulated NO donors, to prolong its effects. This study evaluated the impact...

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Main Authors: Ana Cristina Preisler, Giovanna Camargo do Carmo, Rafael Caetano da Silva, Ana Luisa de Oliveira Simões, Juliana de Carvalho Izidoro, Joana Claudio Pieretti, Roberta Albino dos Reis, André Luiz Floriano Jacob, Amedea Barozzi Seabra, Halley Caixeta Oliveira
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Language:English
Published: MDPI AG 2024-12-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/1/17
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author Ana Cristina Preisler
Giovanna Camargo do Carmo
Rafael Caetano da Silva
Ana Luisa de Oliveira Simões
Juliana de Carvalho Izidoro
Joana Claudio Pieretti
Roberta Albino dos Reis
André Luiz Floriano Jacob
Amedea Barozzi Seabra
Halley Caixeta Oliveira
author_facet Ana Cristina Preisler
Giovanna Camargo do Carmo
Rafael Caetano da Silva
Ana Luisa de Oliveira Simões
Juliana de Carvalho Izidoro
Joana Claudio Pieretti
Roberta Albino dos Reis
André Luiz Floriano Jacob
Amedea Barozzi Seabra
Halley Caixeta Oliveira
author_sort Ana Cristina Preisler
collection DOAJ
description Nitric oxide (NO) is a multifunctional signaling molecule in plants, playing key roles in germination, microbial symbiosis, and nodule formation. However, its instability requires innovative approaches, such as using nanoencapsulated NO donors, to prolong its effects. This study evaluated the impact of treating soybean (<i>Glycine max</i>) seeds with the NO donor S-nitrosoglutathione (GSNO), encapsulated in polymeric nanoparticles, on the germination, nodulation, and plant growth. Seeds were treated with free GSNO, chitosan nanoparticles with/without NO (NP CS-GSNO/NP CS-GSH, where GSH is glutathione, the NO donor precursor), and alginate nanoparticles with/without NO (NP Al-GSNO/NP Al-GSH). Chitosan nanoparticles (positive zeta potential) were smaller and released NO faster compared with alginate nanoparticles (negative zeta potential). The seed treatment with NP CS-GSNO (1 mM, related to GSNO concentration) significantly improved germination percentage, root length, number of secondary roots, and dry root mass of soybean compared with the control. Conversely, NP CS-GSH resulted in decreased root and shoot length. NP Al-GSNO enhanced shoot dry mass and increased the number of secondary roots by approximately threefold at the highest concentrations. NP CS-GSNO, NP Al-GSNO, and NP Al-GSH increased S-nitrosothiol levels in the roots by approximately fourfold compared with the control. However, NP CS-GSNO was the only treatment that increased the nodule dry mass of soybean plants. Therefore, our results indicate the potential of chitosan nanoparticles to improve the application of NO donors in soybean seeds.
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spelling doaj-art-6e8db733daf04147851322795d9a52642025-01-10T13:19:29ZengMDPI AGPlants2223-77472024-12-011411710.3390/plants14010017Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric NanoparticlesAna Cristina Preisler0Giovanna Camargo do Carmo1Rafael Caetano da Silva2Ana Luisa de Oliveira Simões3Juliana de Carvalho Izidoro4Joana Claudio Pieretti5Roberta Albino dos Reis6André Luiz Floriano Jacob7Amedea Barozzi Seabra8Halley Caixeta Oliveira9Department of Animal and Plant Biology, Londrina State University, Londrina 86057-970, PR, BrazilDepartment of Animal and Plant Biology, Londrina State University, Londrina 86057-970, PR, BrazilDepartment of Biodiversity Conservation, Institute of Environmental Research, São Paulo 04301-902, SP, BrazilDepartment of Animal and Plant Biology, Londrina State University, Londrina 86057-970, PR, BrazilCenter for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André 09210-580, SP, BrazilCenter for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André 09210-580, SP, BrazilCenter for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André 09210-580, SP, BrazilCenter for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André 09210-580, SP, BrazilCenter for Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), Santo André 09210-580, SP, BrazilDepartment of Animal and Plant Biology, Londrina State University, Londrina 86057-970, PR, BrazilNitric oxide (NO) is a multifunctional signaling molecule in plants, playing key roles in germination, microbial symbiosis, and nodule formation. However, its instability requires innovative approaches, such as using nanoencapsulated NO donors, to prolong its effects. This study evaluated the impact of treating soybean (<i>Glycine max</i>) seeds with the NO donor S-nitrosoglutathione (GSNO), encapsulated in polymeric nanoparticles, on the germination, nodulation, and plant growth. Seeds were treated with free GSNO, chitosan nanoparticles with/without NO (NP CS-GSNO/NP CS-GSH, where GSH is glutathione, the NO donor precursor), and alginate nanoparticles with/without NO (NP Al-GSNO/NP Al-GSH). Chitosan nanoparticles (positive zeta potential) were smaller and released NO faster compared with alginate nanoparticles (negative zeta potential). The seed treatment with NP CS-GSNO (1 mM, related to GSNO concentration) significantly improved germination percentage, root length, number of secondary roots, and dry root mass of soybean compared with the control. Conversely, NP CS-GSH resulted in decreased root and shoot length. NP Al-GSNO enhanced shoot dry mass and increased the number of secondary roots by approximately threefold at the highest concentrations. NP CS-GSNO, NP Al-GSNO, and NP Al-GSH increased S-nitrosothiol levels in the roots by approximately fourfold compared with the control. However, NP CS-GSNO was the only treatment that increased the nodule dry mass of soybean plants. Therefore, our results indicate the potential of chitosan nanoparticles to improve the application of NO donors in soybean seeds.https://www.mdpi.com/2223-7747/14/1/17nanocarriersnoduleS-nitrosoglutathionenitric oxidechitosan nanoparticlesalginate nanoparticles
spellingShingle Ana Cristina Preisler
Giovanna Camargo do Carmo
Rafael Caetano da Silva
Ana Luisa de Oliveira Simões
Juliana de Carvalho Izidoro
Joana Claudio Pieretti
Roberta Albino dos Reis
André Luiz Floriano Jacob
Amedea Barozzi Seabra
Halley Caixeta Oliveira
Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric Nanoparticles
Plants
nanocarriers
nodule
S-nitrosoglutathione
nitric oxide
chitosan nanoparticles
alginate nanoparticles
title Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric Nanoparticles
title_full Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric Nanoparticles
title_fullStr Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric Nanoparticles
title_full_unstemmed Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric Nanoparticles
title_short Improving Soybean Germination and Nodule Development with Nitric Oxide-Releasing Polymeric Nanoparticles
title_sort improving soybean germination and nodule development with nitric oxide releasing polymeric nanoparticles
topic nanocarriers
nodule
S-nitrosoglutathione
nitric oxide
chitosan nanoparticles
alginate nanoparticles
url https://www.mdpi.com/2223-7747/14/1/17
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