Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media
Survival rate of head and neck squamous cell carcinomas (HNSCC) patients are still to date very poor, and the application of innovative clinical approaches are urgently needed. Cold atmospheric plasmas (CAPs) are partially ionized gases that have shown anti-tumor effectiveness over a wide range of c...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024174890 |
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| author | Viviana di Giacomo Marwa Balaha Asia Pece Ilaria Cela Gianluca Fulgenzi Giovanna Orsini Tatiana Spadoni Tirtha Raj Acharya Nagendra Kumar Kaushik Eun Ha Choi Monica Rapino Mariangela Mazzone Gabriella Mincione Gianluca Sala Eloisa Sardella Vittoria Perrotti |
| author_facet | Viviana di Giacomo Marwa Balaha Asia Pece Ilaria Cela Gianluca Fulgenzi Giovanna Orsini Tatiana Spadoni Tirtha Raj Acharya Nagendra Kumar Kaushik Eun Ha Choi Monica Rapino Mariangela Mazzone Gabriella Mincione Gianluca Sala Eloisa Sardella Vittoria Perrotti |
| author_sort | Viviana di Giacomo |
| collection | DOAJ |
| description | Survival rate of head and neck squamous cell carcinomas (HNSCC) patients are still to date very poor, and the application of innovative clinical approaches are urgently needed. Cold atmospheric plasmas (CAPs) are partially ionized gases that have shown anti-tumor effectiveness over a wide range of cancer types with potential application into clinics. However, the comprehension of the mechanisms underlying indirect CAP effects plays a key role for the prediction of treatment outcomes. In our work, we assessed the potential application of indirect CAP, by using plasma activated media (PAM) and plasma-treated liquids (PTL), as therapeutic strategies for HNSCC treatment. The impact of PAM obtained from exposure to CAP for different times was evaluated in three head and neck cancer (HNC) cell lines (HSC3, FaDu, CAL-27). Cytotoxic effects as inhibition of proliferation, apoptosis rate and cell cycle modifications were tested for the different PAM, showing a time- and cell culture media-dependence tightly related to the chemical composition of PAM considered. In addition, cytotoxic effects were also observed on HNC, two bladder cancer models and one breast cancer cell line when considering PTL, paving the way for their application into a clinical setting. |
| format | Article |
| id | doaj-art-6d9225d07d8f4caba8811910b0dec635 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-6d9225d07d8f4caba8811910b0dec6352025-01-17T04:51:20ZengElsevierHeliyon2405-84402025-01-01111e41458Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture mediaViviana di Giacomo0Marwa Balaha1Asia Pece2Ilaria Cela3Gianluca Fulgenzi4Giovanna Orsini5Tatiana Spadoni6Tirtha Raj Acharya7Nagendra Kumar Kaushik8Eun Ha Choi9Monica Rapino10Mariangela Mazzone11Gabriella Mincione12Gianluca Sala13Eloisa Sardella14Vittoria Perrotti15Department of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; UdA-TechLab, Research Center, “G. d’Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kafrelsheikh University, 33516, Kafr El Sheikh, EgyptDepartment of Innovative Technologies in Medicine & Dentistry, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Clinical and Molecular Sciences, Polytechnic University of Marche, Ancona, ItalyDepartment of Clinical Sciences and Stomatology (DISCO), Polytechnic University of Marche, Ancona, ItalyDepartment of Clinical and Molecular Sciences, Polytechnic University of Marche, Ancona, ItalyPlasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, South KoreaPlasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, South KoreaPlasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, South KoreaGenetic Molecular Institute of CNR, Unit of Chieti, “G. d’Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, “G. d’Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, “G. d’Annunzio” University of Chieti-Pescara, Chieti, ItalyDepartment of Innovative Technologies in Medicine & Dentistry, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, Chieti, ItalyCNR- Istituto di Nanotecnologia (CNR-NANOTEC) UoS Bari, c/o Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, via Orabona, 4, 70126, Bari, ItalyUdA-TechLab, Research Center, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; Department of Innovative Technologies in Medicine & Dentistry, “G. d’Annunzio” University of Chieti-Pescara, Chieti, Italy; Corresponding author. Department of Innovative Technologies in Medicine & Dentistry, University ''G. d'Annunzio'', Chieti-Pescara, Via dei vestini 31, 66100, Chieti, Italy.Survival rate of head and neck squamous cell carcinomas (HNSCC) patients are still to date very poor, and the application of innovative clinical approaches are urgently needed. Cold atmospheric plasmas (CAPs) are partially ionized gases that have shown anti-tumor effectiveness over a wide range of cancer types with potential application into clinics. However, the comprehension of the mechanisms underlying indirect CAP effects plays a key role for the prediction of treatment outcomes. In our work, we assessed the potential application of indirect CAP, by using plasma activated media (PAM) and plasma-treated liquids (PTL), as therapeutic strategies for HNSCC treatment. The impact of PAM obtained from exposure to CAP for different times was evaluated in three head and neck cancer (HNC) cell lines (HSC3, FaDu, CAL-27). Cytotoxic effects as inhibition of proliferation, apoptosis rate and cell cycle modifications were tested for the different PAM, showing a time- and cell culture media-dependence tightly related to the chemical composition of PAM considered. In addition, cytotoxic effects were also observed on HNC, two bladder cancer models and one breast cancer cell line when considering PTL, paving the way for their application into a clinical setting.http://www.sciencedirect.com/science/article/pii/S2405844024174890Cold atmospheric plasmaPlasma activated mediaApoptosisCell cycleCell proliferationHead and neck cancer |
| spellingShingle | Viviana di Giacomo Marwa Balaha Asia Pece Ilaria Cela Gianluca Fulgenzi Giovanna Orsini Tatiana Spadoni Tirtha Raj Acharya Nagendra Kumar Kaushik Eun Ha Choi Monica Rapino Mariangela Mazzone Gabriella Mincione Gianluca Sala Eloisa Sardella Vittoria Perrotti Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media Heliyon Cold atmospheric plasma Plasma activated media Apoptosis Cell cycle Cell proliferation Head and neck cancer |
| title | Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media |
| title_full | Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media |
| title_fullStr | Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media |
| title_full_unstemmed | Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media |
| title_short | Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media |
| title_sort | human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media |
| topic | Cold atmospheric plasma Plasma activated media Apoptosis Cell cycle Cell proliferation Head and neck cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024174890 |
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