Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea model
Abstract Scientific bodies overseeing UV radiation protection recommend safety limits for exposure to ultraviolet radiation in the workplace based on published peer-reviewed data. To support this goal, a 3D model of the human cornea was used to assess the wavelength dependence of corneal damage indu...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-84196-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559739395211264 |
|---|---|
| author | Manuela Buonanno Raabia Hashmi Camryn E. Petersen Zheng Tang David Welch Igor Shuryak David J. Brenner |
| author_facet | Manuela Buonanno Raabia Hashmi Camryn E. Petersen Zheng Tang David Welch Igor Shuryak David J. Brenner |
| author_sort | Manuela Buonanno |
| collection | DOAJ |
| description | Abstract Scientific bodies overseeing UV radiation protection recommend safety limits for exposure to ultraviolet radiation in the workplace based on published peer-reviewed data. To support this goal, a 3D model of the human cornea was used to assess the wavelength dependence of corneal damage induced by UV-C radiation. In the first set of experiments the models were exposed with or without simulated tears; at each wavelength (215–255 nm) cells with DNA dimers and their distribution within the epithelium were measured. Simulated tears reduced the fraction of damaged cells to an extent dependent on the wavelength and tissue layer. Subsequent experiments were performed with models exposed without simulated tears; yields of DNA-damaged cells and their distribution within the corneal epithelium were evaluated at each wavelength, together with other markers of cell and tissue integrity. Unlike relatively longer wavelengths, the range of wavelengths commonly referred to as far-UV-C (215–235 nm) only induced dimers in the uppermost layers of the epithelium and did not result in lasting damage or halt proliferation of the germinative cells. These results provide evidence for the recommended exposure limits for far-UV-C wavelengths, which have been proposed as a practical technology to reduce the risk of transmission of airborne diseases in occupied locations. |
| format | Article |
| id | doaj-art-6ae5b6ab1ea64e999f682fd32f28fd8d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-6ae5b6ab1ea64e999f682fd32f28fd8d2025-01-05T12:13:27ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-84196-4Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea modelManuela Buonanno0Raabia Hashmi1Camryn E. Petersen2Zheng Tang3David Welch4Igor Shuryak5David J. Brenner6Center for Radiological Research, Columbia University Irving Medical CenterCenter for Radiological Research, Columbia University Irving Medical CenterCenter for Radiological Research, Columbia University Irving Medical CenterCenter for Radiological Research, Columbia University Irving Medical CenterCenter for Radiological Research, Columbia University Irving Medical CenterCenter for Radiological Research, Columbia University Irving Medical CenterCenter for Radiological Research, Columbia University Irving Medical CenterAbstract Scientific bodies overseeing UV radiation protection recommend safety limits for exposure to ultraviolet radiation in the workplace based on published peer-reviewed data. To support this goal, a 3D model of the human cornea was used to assess the wavelength dependence of corneal damage induced by UV-C radiation. In the first set of experiments the models were exposed with or without simulated tears; at each wavelength (215–255 nm) cells with DNA dimers and their distribution within the epithelium were measured. Simulated tears reduced the fraction of damaged cells to an extent dependent on the wavelength and tissue layer. Subsequent experiments were performed with models exposed without simulated tears; yields of DNA-damaged cells and their distribution within the corneal epithelium were evaluated at each wavelength, together with other markers of cell and tissue integrity. Unlike relatively longer wavelengths, the range of wavelengths commonly referred to as far-UV-C (215–235 nm) only induced dimers in the uppermost layers of the epithelium and did not result in lasting damage or halt proliferation of the germinative cells. These results provide evidence for the recommended exposure limits for far-UV-C wavelengths, which have been proposed as a practical technology to reduce the risk of transmission of airborne diseases in occupied locations.https://doi.org/10.1038/s41598-024-84196-4 |
| spellingShingle | Manuela Buonanno Raabia Hashmi Camryn E. Petersen Zheng Tang David Welch Igor Shuryak David J. Brenner Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea model Scientific Reports |
| title | Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea model |
| title_full | Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea model |
| title_fullStr | Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea model |
| title_full_unstemmed | Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea model |
| title_short | Wavelength-dependent DNA damage induced by single wavelengths of UV-C radiation (215 to 255 nm) in a human cornea model |
| title_sort | wavelength dependent dna damage induced by single wavelengths of uv c radiation 215 to 255 nm in a human cornea model |
| url | https://doi.org/10.1038/s41598-024-84196-4 |
| work_keys_str_mv | AT manuelabuonanno wavelengthdependentdnadamageinducedbysinglewavelengthsofuvcradiation215to255nminahumancorneamodel AT raabiahashmi wavelengthdependentdnadamageinducedbysinglewavelengthsofuvcradiation215to255nminahumancorneamodel AT camrynepetersen wavelengthdependentdnadamageinducedbysinglewavelengthsofuvcradiation215to255nminahumancorneamodel AT zhengtang wavelengthdependentdnadamageinducedbysinglewavelengthsofuvcradiation215to255nminahumancorneamodel AT davidwelch wavelengthdependentdnadamageinducedbysinglewavelengthsofuvcradiation215to255nminahumancorneamodel AT igorshuryak wavelengthdependentdnadamageinducedbysinglewavelengthsofuvcradiation215to255nminahumancorneamodel AT davidjbrenner wavelengthdependentdnadamageinducedbysinglewavelengthsofuvcradiation215to255nminahumancorneamodel |