Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencing
BackgroundAlthough previous studies have reported the dysregulation of respiratory tract microbiota in infectious diseases, insufficient data exist regarding respiratory microbiota imbalances in the lower respiratory tracts of children with pulmonary tuberculosis (PTB). In this study, we assessed th...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1492881/full |
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| author | Haiyi Zhou Haiyi Zhou Yi Pei Yi Pei Qifang Xie Qifang Xie Wenjie Nie Wenjie Nie Xiaoyan Liu Xiaoyan Liu Han Xia Jie Jiang Jie Jiang Jie Jiang Jie Jiang |
| author_facet | Haiyi Zhou Haiyi Zhou Yi Pei Yi Pei Qifang Xie Qifang Xie Wenjie Nie Wenjie Nie Xiaoyan Liu Xiaoyan Liu Han Xia Jie Jiang Jie Jiang Jie Jiang Jie Jiang |
| author_sort | Haiyi Zhou |
| collection | DOAJ |
| description | BackgroundAlthough previous studies have reported the dysregulation of respiratory tract microbiota in infectious diseases, insufficient data exist regarding respiratory microbiota imbalances in the lower respiratory tracts of children with pulmonary tuberculosis (PTB). In this study, we assessed the value of mNGS in the pathogen diagnosis and microbiome analysis of PTB patients using bronchoalveolar lavage fluid (BALF) samples.MethodsA total of 64 participants, comprising 43 pediatric PTB and 21 pediatric pneumonia patients were recruited in the present study. BALF samples were collected from the above participants. Parallel comparisons between mNGS and conventional microbial test (CMT) pathogen detection were performed. Moreover, the diversity and structure of all 64 patients’ lung BALF microbiomes were explored using the mNGS data.ResultsComparing to the final clinical diagnosis, mNGS in BALF samples produced a sensitivity of 46.51%, which was lower than that of TB-PCR (55.00%) and Xpert (55.00%). The diagnostic efficacy of PTB can be highly enhanced by mNGS combined with TB-PCR (AUC=0.8140, P<0.0001). There were no significant differences in the diversity either between patients with TB and pneumonia. Positive mNGS pathogen results in pediatric PTB patients significantly affect the β-diversity of the pulmonary microbiota. In addition, significant taxonomic differences were found in BALF specimens from patients with PTB and pneumonia, both of which have unique bacterial compositions.ConclusionsmNGS is valuable in the etiological diagnosis of PTB, and can reveal pulmonary microecological characteristics. For pediatric PTB patients, the mNGS should be implemented early and complementary to CMTs. |
| format | Article |
| id | doaj-art-68d565e28e3b47f8ac054f2af8b24ca0 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-68d565e28e3b47f8ac054f2af8b24ca02024-12-19T05:10:07ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-12-011410.3389/fcimb.2024.14928811492881Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencingHaiyi Zhou0Haiyi Zhou1Yi Pei2Yi Pei3Qifang Xie4Qifang Xie5Wenjie Nie6Wenjie Nie7Xiaoyan Liu8Xiaoyan Liu9Han Xia10Jie Jiang11Jie Jiang12Jie Jiang13Jie Jiang14The Affiliated Changsha Central Hospital, Department of Center for Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, ChinaChangsha Tuberculosis Technology Innovation Center of Children, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaThe Affiliated Changsha Central Hospital, Department of Center for Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, ChinaHunan Clinical Medical Technology Demonstration Base of Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaThe Affiliated Changsha Central Hospital, Department of Center for Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, ChinaChangsha Tuberculosis Technology Innovation Center of Children, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaThe Affiliated Changsha Central Hospital, Department of Center for Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, ChinaChangsha Tuberculosis Technology Innovation Center of Children, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaThe Affiliated Changsha Central Hospital, Department of Center for Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, ChinaChangsha Tuberculosis Technology Innovation Center of Children, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaDepartment of Scientific Affairs, Hugobiotech Co., Ltd., Beijing, ChinaThe Affiliated Changsha Central Hospital, Department of Center for Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, ChinaChangsha Tuberculosis Technology Innovation Center of Children, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaHunan Clinical Medical Technology Demonstration Base of Tuberculosis Diagnosis and Treatment, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaKey Laboratory of Rare Pediatric Diseases, Ministry of Education, Hengyang Medical School, University of South China, Changsha, ChinaBackgroundAlthough previous studies have reported the dysregulation of respiratory tract microbiota in infectious diseases, insufficient data exist regarding respiratory microbiota imbalances in the lower respiratory tracts of children with pulmonary tuberculosis (PTB). In this study, we assessed the value of mNGS in the pathogen diagnosis and microbiome analysis of PTB patients using bronchoalveolar lavage fluid (BALF) samples.MethodsA total of 64 participants, comprising 43 pediatric PTB and 21 pediatric pneumonia patients were recruited in the present study. BALF samples were collected from the above participants. Parallel comparisons between mNGS and conventional microbial test (CMT) pathogen detection were performed. Moreover, the diversity and structure of all 64 patients’ lung BALF microbiomes were explored using the mNGS data.ResultsComparing to the final clinical diagnosis, mNGS in BALF samples produced a sensitivity of 46.51%, which was lower than that of TB-PCR (55.00%) and Xpert (55.00%). The diagnostic efficacy of PTB can be highly enhanced by mNGS combined with TB-PCR (AUC=0.8140, P<0.0001). There were no significant differences in the diversity either between patients with TB and pneumonia. Positive mNGS pathogen results in pediatric PTB patients significantly affect the β-diversity of the pulmonary microbiota. In addition, significant taxonomic differences were found in BALF specimens from patients with PTB and pneumonia, both of which have unique bacterial compositions.ConclusionsmNGS is valuable in the etiological diagnosis of PTB, and can reveal pulmonary microecological characteristics. For pediatric PTB patients, the mNGS should be implemented early and complementary to CMTs.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1492881/fullpediatric pulmonary tuberculosisbronchoalveolar lavage fluidmetagenomic next-generation sequencingmicroecologyconventional microbial test |
| spellingShingle | Haiyi Zhou Haiyi Zhou Yi Pei Yi Pei Qifang Xie Qifang Xie Wenjie Nie Wenjie Nie Xiaoyan Liu Xiaoyan Liu Han Xia Jie Jiang Jie Jiang Jie Jiang Jie Jiang Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencing Frontiers in Cellular and Infection Microbiology pediatric pulmonary tuberculosis bronchoalveolar lavage fluid metagenomic next-generation sequencing microecology conventional microbial test |
| title | Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencing |
| title_full | Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencing |
| title_fullStr | Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencing |
| title_full_unstemmed | Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencing |
| title_short | Diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next-generation sequencing |
| title_sort | diagnosis and insight into the unique lung microbiota of pediatric pulmonary tuberculosis patients by bronchoalveolar lavage using metagenomic next generation sequencing |
| topic | pediatric pulmonary tuberculosis bronchoalveolar lavage fluid metagenomic next-generation sequencing microecology conventional microbial test |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1492881/full |
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