过氧化氢酶对高糖诱导NIT-1胰岛β细胞Pax6基因表达下降的影响
【目的】探讨过氧化氢酶(eatalase)对高糖诱导的NIT-1胰岛B细胞成对同源异形盒转录因子6(Pax6)基因表达下降的影响。【方法】体外培养NIT-1胰岛B细胞24h,分4个处理组:低糖组(NG),低糖+过氧化氢酶组(NG+C),高糖组(FIG),高糖+过氧化氢酶组(HG+C),用2,7二氯氢化荧光素二酯(H2DCF-DA)染色检测活性氧簇(ROS)的水平,RT-PCR半定量检测Pax6 mRNA表达。【结果】HG组ROS水平(1.10±0.24)明显高于NG组(0.95±0.26),P〈0.05;FIG+C组ROS水平(0.94±0.26)低于HG组,P〈0.05;HG组Pax6 mR...
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Main Authors: | , , , , |
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Format: | Article |
Language: | zho |
Published: |
Editorial Office of Journal of Sun Yat-sen University
2006-01-01
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Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
Subjects: | |
Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43583029/ |
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