Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats
Abstract To illustrate the anti-diabetic properties of Berberis orthobotrys seeds was the aim of the current study. After a series of experiments, two doses of aqueous methanolic extract of the seeds were selected i.e., 151 mg/kg and 301 mg/kg to assess the anti-diabetic potential in normoglycemic,...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83987-z |
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| author | Ali Imran Abid Hira Muzammel Mohsin Javed Muhammad Muntazir Iqbal Sajid Mahmood Ammar Zidan Fadi Jaber K. K. Mujeeb Rahman Ali Bahadur Shahid Iqbal Muhammad Saad Ayed A. Dera Yousif A. Asiri Abd-ElAziem Farouk Salman Aloufi |
| author_facet | Ali Imran Abid Hira Muzammel Mohsin Javed Muhammad Muntazir Iqbal Sajid Mahmood Ammar Zidan Fadi Jaber K. K. Mujeeb Rahman Ali Bahadur Shahid Iqbal Muhammad Saad Ayed A. Dera Yousif A. Asiri Abd-ElAziem Farouk Salman Aloufi |
| author_sort | Ali Imran Abid |
| collection | DOAJ |
| description | Abstract To illustrate the anti-diabetic properties of Berberis orthobotrys seeds was the aim of the current study. After a series of experiments, two doses of aqueous methanolic extract of the seeds were selected i.e., 151 mg/kg and 301 mg/kg to assess the anti-diabetic potential in normoglycemic, alloxan-induced and glucose-loaded diabetic rats. The effect of the seeds extract on total cholesterol and triglycerides was also assessed. Histological examination of the pancreas and the GC-MS analysis of blood and urine samples of rats were also carried out. At 151 mg/kg and 301 mg/kg doses, the aqueous methanolic extracts of Berberis orthobotrys seeds showed promising results for their anti-diabetic potential comparable to Glibenclamide. Studies on the phytochemistry of AMEBO also exposed the existence of alkaloids, steroids, glycosides, flavonoids and resins. Serum lipid measurements showed a substantial reduction in triglycerides and total cholesterol on days 7 and 15. The pancreas’s islets were protected by the extract, as shown by a histological analysis. The primary metabolite Berberine, which was qualitatively detected by GC-MS in blood and urine samples of rats, may be the alkaloid in command of insulin release or recovery of beta cells after alloxan destruction. More study is necessary to comprehend the underlying signaling process. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-66e7e1519737401eac3071e491f6e6882025-01-05T12:16:53ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-024-83987-zUnveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic ratsAli Imran Abid0Hira Muzammel1Mohsin Javed2Muhammad Muntazir Iqbal3Sajid Mahmood4Ammar Zidan5Fadi Jaber6K. K. Mujeeb Rahman7Ali Bahadur8Shahid Iqbal9Muhammad Saad10Ayed A. Dera11Yousif A. Asiri12Abd-ElAziem Farouk13Salman Aloufi14Department of Pharmacology, Faculty of Pharmacy, The University of LahoreDepartment of Pharmacology, Faculty of Pharmacy, Lahore College for Women UniversityDepartment of Chemistry, School of Science, University of Management and TechnologyDepartment of Chemistry, School of Science, University of Management and TechnologyNottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo ChinaBiomedical Engineering Department, College of Engineering and Technologies, Al-Mustaqbal UniversityDepartment of Biomedical Engineering, Ajman UniversityDepartment of Biomedical Engineering, Ajman UniversityNanomaterials Research Center, Department of Chemistry, College of Science, Mathematics, and Technology, Wenzhou-Kean UniversityNottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo ChinaCentre for Organic and Nanohybrid Electronics, Silesian University of TechnologyDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid UniversityTaif UniversityDepartment of Biotechnology, College of Science, Taif UniversityDepartment of Biotechnology, College of Science, Taif UniversityAbstract To illustrate the anti-diabetic properties of Berberis orthobotrys seeds was the aim of the current study. After a series of experiments, two doses of aqueous methanolic extract of the seeds were selected i.e., 151 mg/kg and 301 mg/kg to assess the anti-diabetic potential in normoglycemic, alloxan-induced and glucose-loaded diabetic rats. The effect of the seeds extract on total cholesterol and triglycerides was also assessed. Histological examination of the pancreas and the GC-MS analysis of blood and urine samples of rats were also carried out. At 151 mg/kg and 301 mg/kg doses, the aqueous methanolic extracts of Berberis orthobotrys seeds showed promising results for their anti-diabetic potential comparable to Glibenclamide. Studies on the phytochemistry of AMEBO also exposed the existence of alkaloids, steroids, glycosides, flavonoids and resins. Serum lipid measurements showed a substantial reduction in triglycerides and total cholesterol on days 7 and 15. The pancreas’s islets were protected by the extract, as shown by a histological analysis. The primary metabolite Berberine, which was qualitatively detected by GC-MS in blood and urine samples of rats, may be the alkaloid in command of insulin release or recovery of beta cells after alloxan destruction. More study is necessary to comprehend the underlying signaling process.https://doi.org/10.1038/s41598-024-83987-zBerberis orthobotrysDiabetes mellitusMetabolic irregularitiesGC-MS |
| spellingShingle | Ali Imran Abid Hira Muzammel Mohsin Javed Muhammad Muntazir Iqbal Sajid Mahmood Ammar Zidan Fadi Jaber K. K. Mujeeb Rahman Ali Bahadur Shahid Iqbal Muhammad Saad Ayed A. Dera Yousif A. Asiri Abd-ElAziem Farouk Salman Aloufi Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats Scientific Reports Berberis orthobotrys Diabetes mellitus Metabolic irregularities GC-MS |
| title | Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats |
| title_full | Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats |
| title_fullStr | Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats |
| title_full_unstemmed | Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats |
| title_short | Unveiling nature’s antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats |
| title_sort | unveiling nature s antidiabetic potential in berberis orthobotrys seeds for alloxan induced diabetic rats |
| topic | Berberis orthobotrys Diabetes mellitus Metabolic irregularities GC-MS |
| url | https://doi.org/10.1038/s41598-024-83987-z |
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