Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells
Abstract This study included patients with thyroid eye disease (n = 81) and Graves’ disease (n = 165) for a cross-sectional study. Then, peripheral blood mononuclear cells (PBMCs) were collected from patients with thyroid eye disease (n = 5), Graves’ disease (n = 15), and normal individuals (n = 5)...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-08-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-08904-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849332896464109568 |
|---|---|
| author | Hongli Lin Honghong Duan Jingyi Zheng Zhengrong Jiang Yajing Xu Huibin Huang |
| author_facet | Hongli Lin Honghong Duan Jingyi Zheng Zhengrong Jiang Yajing Xu Huibin Huang |
| author_sort | Hongli Lin |
| collection | DOAJ |
| description | Abstract This study included patients with thyroid eye disease (n = 81) and Graves’ disease (n = 165) for a cross-sectional study. Then, peripheral blood mononuclear cells (PBMCs) were collected from patients with thyroid eye disease (n = 5), Graves’ disease (n = 15), and normal individuals (n = 5) for RNA-seq. WGCNA analysis was conducted on the RNA-seq results to identify genes related to thyroid eye disease, and these genes were verified by RT-qPCR. The results suggest that: (1) Neutrophil counts were elevated in the TED group compared to the GD group. (2) Ten hub genes, MMP9, BPI, CD177, MPO, CEACAM8, CEACAM1, SLPI, AZU1, KCNJ15 and TNFRSF10C, were identified. The above hub genes were positively correlated with neutrophils. |
| format | Article |
| id | doaj-art-65fedcad5e4e413eab29ba79754c20db |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-65fedcad5e4e413eab29ba79754c20db2025-08-20T03:46:04ZengNature PortfolioScientific Reports2045-23222025-08-0115111010.1038/s41598-025-08904-4Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cellsHongli Lin0Honghong Duan1Jingyi Zheng2Zhengrong Jiang3Yajing Xu4Huibin Huang5Department of Endocrinology, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Gynaecology and Obstetrics, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Endocrinology, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Endocrinology, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Endocrinology, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Endocrinology, The Second Affiliated Hospital of Fujian Medical UniversityAbstract This study included patients with thyroid eye disease (n = 81) and Graves’ disease (n = 165) for a cross-sectional study. Then, peripheral blood mononuclear cells (PBMCs) were collected from patients with thyroid eye disease (n = 5), Graves’ disease (n = 15), and normal individuals (n = 5) for RNA-seq. WGCNA analysis was conducted on the RNA-seq results to identify genes related to thyroid eye disease, and these genes were verified by RT-qPCR. The results suggest that: (1) Neutrophil counts were elevated in the TED group compared to the GD group. (2) Ten hub genes, MMP9, BPI, CD177, MPO, CEACAM8, CEACAM1, SLPI, AZU1, KCNJ15 and TNFRSF10C, were identified. The above hub genes were positively correlated with neutrophils.https://doi.org/10.1038/s41598-025-08904-4Thyroid eye diseaseGraves’ diseaseWGCNA (weighted gene co-expression network analysis)NeutrophilRNA-seq |
| spellingShingle | Hongli Lin Honghong Duan Jingyi Zheng Zhengrong Jiang Yajing Xu Huibin Huang Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells Scientific Reports Thyroid eye disease Graves’ disease WGCNA (weighted gene co-expression network analysis) Neutrophil RNA-seq |
| title | Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells |
| title_full | Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells |
| title_fullStr | Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells |
| title_full_unstemmed | Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells |
| title_short | Clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells |
| title_sort | clinical characteristics of thyroid eye disease and expression profile of peripheral blood immune cells |
| topic | Thyroid eye disease Graves’ disease WGCNA (weighted gene co-expression network analysis) Neutrophil RNA-seq |
| url | https://doi.org/10.1038/s41598-025-08904-4 |
| work_keys_str_mv | AT honglilin clinicalcharacteristicsofthyroideyediseaseandexpressionprofileofperipheralbloodimmunecells AT honghongduan clinicalcharacteristicsofthyroideyediseaseandexpressionprofileofperipheralbloodimmunecells AT jingyizheng clinicalcharacteristicsofthyroideyediseaseandexpressionprofileofperipheralbloodimmunecells AT zhengrongjiang clinicalcharacteristicsofthyroideyediseaseandexpressionprofileofperipheralbloodimmunecells AT yajingxu clinicalcharacteristicsofthyroideyediseaseandexpressionprofileofperipheralbloodimmunecells AT huibinhuang clinicalcharacteristicsofthyroideyediseaseandexpressionprofileofperipheralbloodimmunecells |