Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 Isolate
African swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia. The causative agent, ASF virus (ASFV), is a large, double-stranded and structurally complex virus. The ASFV genome encodes for more than 160 proteins; however, the...
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| author | Elizabeth Ramirez-Medina Lauro Velazquez-Salinas Alyssa Valladares Ediane Silva Leeanna Burton Douglas P. Gladue Manuel V. Borca |
| author_facet | Elizabeth Ramirez-Medina Lauro Velazquez-Salinas Alyssa Valladares Ediane Silva Leeanna Burton Douglas P. Gladue Manuel V. Borca |
| author_sort | Elizabeth Ramirez-Medina |
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| description | African swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia. The causative agent, ASF virus (ASFV), is a large, double-stranded and structurally complex virus. The ASFV genome encodes for more than 160 proteins; however, the functions of most of these proteins are still in the process of being characterized. The ASF gene <i>R298L</i>, which has previously been characterized as able to encode a functional serine protein kinase, is expressed late in the virus infection cycle and may be part of the virus particle. There is no description of the importance of the <i>R298L</i> gene in basic virus functions such as replication or virulence in the natural host. Based on its evolution, it is proposed that there are four different phenotypes of <i>R298L</i> of ASFV in nature, which may have potential implications for <i>R298L</i> functionality. We report here that a recombinant virus lacking the <i>R298L</i> gene in the Georgia 2010 isolate, ASFV-G-∆R298L, does not exhibit significant changes in its replication in primary cultures of swine macrophages. In addition, when experimentally inoculated in pigs, ASFV-G-∆R298L induced a fatal form of the disease similar to that caused by the parental virulent ASFV-G. Therefore, deletion of <i>R298L</i> does not significantly affect virus replication and virulence in domestic pigs of the ASFV Georgia 2010 isolate. |
| format | Article |
| id | doaj-art-63bec5bfeb1b4b7abbd95a55db93f60b |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-63bec5bfeb1b4b7abbd95a55db93f60b2024-12-27T14:59:13ZengMDPI AGViruses1999-49152024-12-011612191110.3390/v16121911Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 IsolateElizabeth Ramirez-Medina0Lauro Velazquez-Salinas1Alyssa Valladares2Ediane Silva3Leeanna Burton4Douglas P. Gladue5Manuel V. Borca6Foreign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAAfrican swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia. The causative agent, ASF virus (ASFV), is a large, double-stranded and structurally complex virus. The ASFV genome encodes for more than 160 proteins; however, the functions of most of these proteins are still in the process of being characterized. The ASF gene <i>R298L</i>, which has previously been characterized as able to encode a functional serine protein kinase, is expressed late in the virus infection cycle and may be part of the virus particle. There is no description of the importance of the <i>R298L</i> gene in basic virus functions such as replication or virulence in the natural host. Based on its evolution, it is proposed that there are four different phenotypes of <i>R298L</i> of ASFV in nature, which may have potential implications for <i>R298L</i> functionality. We report here that a recombinant virus lacking the <i>R298L</i> gene in the Georgia 2010 isolate, ASFV-G-∆R298L, does not exhibit significant changes in its replication in primary cultures of swine macrophages. In addition, when experimentally inoculated in pigs, ASFV-G-∆R298L induced a fatal form of the disease similar to that caused by the parental virulent ASFV-G. Therefore, deletion of <i>R298L</i> does not significantly affect virus replication and virulence in domestic pigs of the ASFV Georgia 2010 isolate.https://www.mdpi.com/1999-4915/16/12/1911ASFVASFAfrican swine fever virusrecombinant virusASFV virulenceASFV <i>R298L</i> gene |
| spellingShingle | Elizabeth Ramirez-Medina Lauro Velazquez-Salinas Alyssa Valladares Ediane Silva Leeanna Burton Douglas P. Gladue Manuel V. Borca Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 Isolate Viruses ASFV ASF African swine fever virus recombinant virus ASFV virulence ASFV <i>R298L</i> gene |
| title | Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 Isolate |
| title_full | Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 Isolate |
| title_fullStr | Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 Isolate |
| title_full_unstemmed | Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 Isolate |
| title_short | Assessment of the Effect of Deleting the African Swine Fever Virus Gene <i>R298L</i> on Virus Replication and Virulence of the Georgia2010 Isolate |
| title_sort | assessment of the effect of deleting the african swine fever virus gene i r298l i on virus replication and virulence of the georgia2010 isolate |
| topic | ASFV ASF African swine fever virus recombinant virus ASFV virulence ASFV <i>R298L</i> gene |
| url | https://www.mdpi.com/1999-4915/16/12/1911 |
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