Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function
Background: Over the last decade, the anticancer effects of Rhus verniciflua Stokes (RVS) have been reported in various preclinical or clinical studies. However, the effects of RVS on immuno-oncology, especially on the functional properties of T cells and their phenotypes, remain unclear. Here, we p...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2025-01-01
|
| Series: | Integrative Cancer Therapies |
| Online Access: | https://doi.org/10.1177/15347354241308220 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841557284133535744 |
|---|---|
| author | Seoyoung Kim PhD Young-Kwan Lee PhD Wang-Jun Lee MD, PhD Hyoun Jong Moon MD, PhD Sanghun Lee KMD, PhD |
| author_facet | Seoyoung Kim PhD Young-Kwan Lee PhD Wang-Jun Lee MD, PhD Hyoun Jong Moon MD, PhD Sanghun Lee KMD, PhD |
| author_sort | Seoyoung Kim PhD |
| collection | DOAJ |
| description | Background: Over the last decade, the anticancer effects of Rhus verniciflua Stokes (RVS) have been reported in various preclinical or clinical studies. However, the effects of RVS on immuno-oncology, especially on the functional properties of T cells and their phenotypes, remain unclear. Here, we planned to investigate the impact of RVS on immuno-oncology, specifically focusing on its effects on T cells. Methods Peripheral blood mononuclear cells (PBMCs) from breast cancer patients were isolated to obtain cytokine-induced killer cell populations with >85% CD3+ T cells. The anticancer activity of these T cells was evaluated by introducing red fluorescent protein (RFP) into HLA-A02:01 type-matched breast cancer cell lines (MCF7 and MDA-MB-231) and analyzing the results using flow cytometry. The effect of RVS extracts on T cell phenotype was assessed using markers such as CTLA-4 and PD-1, as well as mRNA expression levels of key genes (IFN-γ, TNF-α, and IL-2). Results RVS treatment significantly enhanced the anticancer activity of T cells against breast cancer cells. Specifically, T cells treated with 100 µg/mL of RVS showed a 20.6% increase in cytotoxicity against MCF-7 cells and a 36.2% increase against MDA-MB231 cells compared to the control. Additionally, RVS treatment led to a significant reduction in PD-1 expression on T cells. Conclusion: Our findings demonstrate that RVS treatment enhances T cell function against breast cancer cells by reducing PD-1 expression. These results suggest that components of RVS may serve as potential candidates for restoring exhausted T cells in cancer therapy. |
| format | Article |
| id | doaj-art-635b360bfbb943d39e3d0baf2ac943e0 |
| institution | Kabale University |
| issn | 1552-695X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Integrative Cancer Therapies |
| spelling | doaj-art-635b360bfbb943d39e3d0baf2ac943e02025-01-06T14:03:59ZengSAGE PublishingIntegrative Cancer Therapies1552-695X2025-01-012410.1177/15347354241308220Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell FunctionSeoyoung Kim PhD0Young-Kwan Lee PhD1Wang-Jun Lee MD, PhD2Hyoun Jong Moon MD, PhD3Sanghun Lee KMD, PhD4Eutilex Co. Ltd., Geumcheon-gu, Seoul, Republic of KoreaExcelsisbio Inc., Goyang-si, Gyeonggi-do, Republic of KoreaMyongji Hospital, Goyang-si, Gyeonggi-do, Republic of KoreaMyongji Hospital, Goyang-si, Gyeonggi-do, Republic of KoreaDepartment of Bioconvergence & Engineering, Dankook University, Yongin-si, Gyeonggi-do, Republic of KoreaBackground: Over the last decade, the anticancer effects of Rhus verniciflua Stokes (RVS) have been reported in various preclinical or clinical studies. However, the effects of RVS on immuno-oncology, especially on the functional properties of T cells and their phenotypes, remain unclear. Here, we planned to investigate the impact of RVS on immuno-oncology, specifically focusing on its effects on T cells. Methods Peripheral blood mononuclear cells (PBMCs) from breast cancer patients were isolated to obtain cytokine-induced killer cell populations with >85% CD3+ T cells. The anticancer activity of these T cells was evaluated by introducing red fluorescent protein (RFP) into HLA-A02:01 type-matched breast cancer cell lines (MCF7 and MDA-MB-231) and analyzing the results using flow cytometry. The effect of RVS extracts on T cell phenotype was assessed using markers such as CTLA-4 and PD-1, as well as mRNA expression levels of key genes (IFN-γ, TNF-α, and IL-2). Results RVS treatment significantly enhanced the anticancer activity of T cells against breast cancer cells. Specifically, T cells treated with 100 µg/mL of RVS showed a 20.6% increase in cytotoxicity against MCF-7 cells and a 36.2% increase against MDA-MB231 cells compared to the control. Additionally, RVS treatment led to a significant reduction in PD-1 expression on T cells. Conclusion: Our findings demonstrate that RVS treatment enhances T cell function against breast cancer cells by reducing PD-1 expression. These results suggest that components of RVS may serve as potential candidates for restoring exhausted T cells in cancer therapy.https://doi.org/10.1177/15347354241308220 |
| spellingShingle | Seoyoung Kim PhD Young-Kwan Lee PhD Wang-Jun Lee MD, PhD Hyoun Jong Moon MD, PhD Sanghun Lee KMD, PhD Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function Integrative Cancer Therapies |
| title | Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function |
| title_full | Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function |
| title_fullStr | Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function |
| title_full_unstemmed | Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function |
| title_short | Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function |
| title_sort | rhus verniciflua stokes inhibits pd 1 expression and induces anticancer effects by enhancing t cell function |
| url | https://doi.org/10.1177/15347354241308220 |
| work_keys_str_mv | AT seoyoungkimphd rhusvernicifluastokesinhibitspd1expressionandinducesanticancereffectsbyenhancingtcellfunction AT youngkwanleephd rhusvernicifluastokesinhibitspd1expressionandinducesanticancereffectsbyenhancingtcellfunction AT wangjunleemdphd rhusvernicifluastokesinhibitspd1expressionandinducesanticancereffectsbyenhancingtcellfunction AT hyounjongmoonmdphd rhusvernicifluastokesinhibitspd1expressionandinducesanticancereffectsbyenhancingtcellfunction AT sanghunleekmdphd rhusvernicifluastokesinhibitspd1expressionandinducesanticancereffectsbyenhancingtcellfunction |