Adapting Next-Generation Sequencing to <i>in Process</i> CRISPR-Cas9 Genome Editing of Recombinant <i>Ac</i>MNPV Vectors: From Shotgun to Tiled-Amplicon Sequencing

Adapting Next-Generation Sequencing to <i>in Process</i> CRISPR-Cas9 Genome Editing of Recombinant <i>Ac</i>MNPV Vectors: From Shotgun to Tiled-Amplicon Sequencing

The alphabaculovirus <i>Autographa californica</i> multiple nucleopolyhedrovirus (<i>Ac</i>MNPV) is the most commonly used virus in the Baculovirus Expression Vector System (BEVS) and has been utilized for the production of many human and veterinary biologics. <i>Ac<...

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Bibliographic Details
Main Authors: Madhuja Chakraborty, Lisa Nielsen, Delaney Nash, Jozef I. Nissimov, Trevor C. Charles, Marc G. Aucoin
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Viruses
Subjects:
CRISPR-Cas9
baculovirus
<i>Ac</i>MNPV
indel mutation
transfection-infection assay
next-generation sequencing
Online Access:https://www.mdpi.com/1999-4915/17/3/437
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