Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot disease
Recently, attention has been shifting toward the perspective of the existence of plants and microbes as a functioning ecological unit. However, studies highlighting the impacts of the microbial community on plant health are still limited. In this study, fungal community (mycobiome) of leaf, root, an...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1422360/full |
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| author | Abiodun Abeeb Azeez Abiodun Abeeb Azeez Daniel Ofeoritse Esiegbuya Adebola Azeez Lateef Adebola Azeez Lateef Fred O. Asiegbu |
| author_facet | Abiodun Abeeb Azeez Abiodun Abeeb Azeez Daniel Ofeoritse Esiegbuya Adebola Azeez Lateef Adebola Azeez Lateef Fred O. Asiegbu |
| author_sort | Abiodun Abeeb Azeez |
| collection | DOAJ |
| description | Recently, attention has been shifting toward the perspective of the existence of plants and microbes as a functioning ecological unit. However, studies highlighting the impacts of the microbial community on plant health are still limited. In this study, fungal community (mycobiome) of leaf, root, and soil of symptomatic leaf-spot diseased (SS) oil palm were compared against asymptomatic (AS) trees using ITS2 rRNA gene metabarcoding. A total of 3,435,417 high-quality sequences were obtained from 29 samples investigated. Out of the 14 phyla identified, Ascomycota and Basidiomycota were the most dominant accounting for 94.2 and 4.7% of the total counts in AS, and 75 and 21.2% in SS, respectively. Neopestalotiopsis is the most abundant genus for AS representing 8.0% of the identified amplicons compared to 2.0% in SS while Peniophora is the most abundant with 8.6% of the identified amplicons for SS compared to 0.1% in AS. The biomarker discovery algorithm LEfSe revealed different taxa signatures for the sample categories, particularly soil samples from asymptomatic trees, which were the most enriched. Network analysis revealed high modularity across all groups, except in root samples. Additionally, a large proportion of the identified keystone species consisted of rare taxa, suggesting potential role in ecosystem functions. Surprisingly both AS and SS leaf samples shared taxa previously associated with oil palm leaf spot disease. The significant abundance of Trichoderma asperellum in the asymptomatic root samples could be further explored as a potential biocontrol agent against oil palm disease. |
| format | Article |
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| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-5ec64aee91dc466c921357e1d2fb5e022024-12-06T06:50:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14223601422360Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot diseaseAbiodun Abeeb Azeez0Abiodun Abeeb Azeez1Daniel Ofeoritse Esiegbuya2Adebola Azeez Lateef3Adebola Azeez Lateef4Fred O. Asiegbu5Department of Forest Sciences, University of Helsinki, Helsinki, FinlandRainforest Research Station, Forestry Research Institute of Nigeria, Ibadan, NigeriaPathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin City, NigeriaDepartment of Forest Sciences, University of Helsinki, Helsinki, FinlandDepartment of Plant Biology, Faculty of Life Sciences, University of Ilorin, Ilorin, NigeriaDepartment of Forest Sciences, University of Helsinki, Helsinki, FinlandRecently, attention has been shifting toward the perspective of the existence of plants and microbes as a functioning ecological unit. However, studies highlighting the impacts of the microbial community on plant health are still limited. In this study, fungal community (mycobiome) of leaf, root, and soil of symptomatic leaf-spot diseased (SS) oil palm were compared against asymptomatic (AS) trees using ITS2 rRNA gene metabarcoding. A total of 3,435,417 high-quality sequences were obtained from 29 samples investigated. Out of the 14 phyla identified, Ascomycota and Basidiomycota were the most dominant accounting for 94.2 and 4.7% of the total counts in AS, and 75 and 21.2% in SS, respectively. Neopestalotiopsis is the most abundant genus for AS representing 8.0% of the identified amplicons compared to 2.0% in SS while Peniophora is the most abundant with 8.6% of the identified amplicons for SS compared to 0.1% in AS. The biomarker discovery algorithm LEfSe revealed different taxa signatures for the sample categories, particularly soil samples from asymptomatic trees, which were the most enriched. Network analysis revealed high modularity across all groups, except in root samples. Additionally, a large proportion of the identified keystone species consisted of rare taxa, suggesting potential role in ecosystem functions. Surprisingly both AS and SS leaf samples shared taxa previously associated with oil palm leaf spot disease. The significant abundance of Trichoderma asperellum in the asymptomatic root samples could be further explored as a potential biocontrol agent against oil palm disease.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1422360/fulloil palmleaf spot diseasemycobiomefungal communitysymptomaticasymptomatic |
| spellingShingle | Abiodun Abeeb Azeez Abiodun Abeeb Azeez Daniel Ofeoritse Esiegbuya Adebola Azeez Lateef Adebola Azeez Lateef Fred O. Asiegbu Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot disease Frontiers in Microbiology oil palm leaf spot disease mycobiome fungal community symptomatic asymptomatic |
| title | Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot disease |
| title_full | Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot disease |
| title_fullStr | Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot disease |
| title_full_unstemmed | Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot disease |
| title_short | Mycobiome analysis of leaf, root, and soil of symptomatic oil palm trees (Elaeis guineensis Jacq.) affected by leaf spot disease |
| title_sort | mycobiome analysis of leaf root and soil of symptomatic oil palm trees elaeis guineensis jacq affected by leaf spot disease |
| topic | oil palm leaf spot disease mycobiome fungal community symptomatic asymptomatic |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1422360/full |
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