A simple and universal quasi-modular cloning system using NEBuilder® HiFi DNA assembly kit

Modular cloning has transformed synthetic biology by enabling rapid assembly of standardized DNA parts, but its effectiveness is often limited to well-characterised model organisms. Here, we expand the utility of the NEBuilder® HiFi DNA Assembly Kit (New England Biolabs) into a quasi-modular system...

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Bibliographic Details
Main Authors: M. Rey Toleco, Mark van der Giezen
Format: Article
Language:English
Published: Taylor & Francis Group 2025-07-01
Series:BioTechniques
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/07366205.2025.2542012
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