Expression profile of cannabinoid receptor 2 in ovine ileum during prenatal development
<b>Aim: </b>Here, we aimed to explore cannabinoid receptor 2 (CB2) expression in ovine ileum during prenatal development using immunohistochemistry.<p> <b>Materials and Methods: </b>We used 20 ileal samples from fetal sheep whose gestational ages range from 63 to 147 da...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1266 |
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| Summary: | <b>Aim: </b>Here, we aimed to explore cannabinoid receptor 2 (CB2) expression
in ovine ileum during prenatal development using immunohistochemistry.<p>
<b>Materials and Methods: </b>We used 20 ileal samples from fetal sheep
whose gestational ages range from 63 to 147 days. To visualize CB2
expression in ileum, we applied labeled Streptavidin Biotin method
to paraffin sections.<p>
<b>Results: </b>The majority of the intestinal epithelium showed a weak
positive reaction for CB2. Interestingly, we observed intense intracytoplasmic
staining in some epithelial cells of both intestinal villi and
crypts. Moreover, CB2 immunoreaction was observed in ganglion
cells in submucosal and myenteric plexus. We also determined positive
staining in smooth muscle cells of lamina and tunica muscularis.
Towards the end of the prenatal period, we found histologically
mature Peyers patches. CB2 staining was detected in the majority of
immune cells forming the follicle and dome region. Follicle associated
epithelium also showed CB2 immunoreaction.<p>
<b>Conclusion: </b>The present study is the first to describe CB2 expression
in fetal sheep ileum. CB2 expression was determined in epithelial
cells, smooth muscles, enteric neurons and Peyers patches in ileum
fetal sheep under physiological conditions. Considering the morphology
of epithelial cells with intense CB2 staining and previous
findings, CB2 may be a possible marker for enteroendocrine cells
during the prenatal period. |
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| ISSN: | 1309-6958 2146-1953 |