Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in Honduras
Poultry bio-mapping helps identify microbial contamination and process optimization opportunities such as sanitary dressing procedures, equipment adjustments, cross-contamination controls, and chemical intervention applications in commercial poultry processing operations. This study focuses on the d...
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MDPI AG
2024-09-01
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| Series: | Poultry |
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| Online Access: | https://www.mdpi.com/2674-1164/3/4/25 |
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| author | Gabriela K. Betancourt-Barszcz Sabrina E. Blandon David A. Vargas Rossy Bueno López Daniela R. Chavez-Velado Angelica Sánchez Valeria Larios Nadira J. Espinoza-Rock Mirian Bueno-Almendarez Marcos X. Sanchez-Plata |
| author_facet | Gabriela K. Betancourt-Barszcz Sabrina E. Blandon David A. Vargas Rossy Bueno López Daniela R. Chavez-Velado Angelica Sánchez Valeria Larios Nadira J. Espinoza-Rock Mirian Bueno-Almendarez Marcos X. Sanchez-Plata |
| author_sort | Gabriela K. Betancourt-Barszcz |
| collection | DOAJ |
| description | Poultry bio-mapping helps identify microbial contamination and process optimization opportunities such as sanitary dressing procedures, equipment adjustments, cross-contamination controls, and chemical intervention applications in commercial poultry processing operations. This study focuses on the development of a preliminary microbiological baseline of microbial indicators, including total viable counts (TVC), Enterobacteriaceae (EB), and the quantification and detection of pathogens such as <i>Salmonella</i> and <i>Campylobacter</i> spp. in four commercial broiler processing facilities in Honduras. Whole chicken and wing rinses were collected from four poultry processing plants at different locations: live receiving, rehanger, post-evisceration, post-chiller, and wings. The MicroSnap<sup>®</sup> system was used for the enumeration of microbial indicators, the BAX<sup>®</sup>-System-SalQuant<sup>®</sup> was used for the quantification of <i>Salmonella</i>, and the BAX<sup>®</sup>-System-CampyQuant™ was used for <i>Campylobacter</i> spp. Negative samples after enumeration were tested with BAX<sup>®</sup>-System <i>Salmonella</i> and BAX<sup>®</sup>-System <i>Campylobacter</i> for prevalence analysis, respectively. The TVC and EB counts were continuously reduced from the live receiving to the post-chiller location, presenting a statistically significant increase (<i>p</i> < 0.01) at the wings location. The <i>Salmonella</i> counts were significantly different between stages (<i>p</i> < 0.01). The prevalence of <i>Salmonella</i> was highest in the live receiving stage, with 92.50%, while that at the post-chiller stage was the lowest, at 15.38%. <i>Campylobacter</i> spp. counts were lower than that at the other stages at wings 1.61 Log CFU/sample; however, <i>Campylobacter</i> spp.’s prevalence was higher than 62.5% in all stages. Microbial bio-mapping using novel technologies suitable for mobile applications was conducted in this study to establish statistical process control parameters for microorganisms. A nationwide microbial baseline for commercial broiler processing facilities in Honduras was developed. In-country data serve as a benchmark for continuous improvement at each facility evaluated and can assist regulatory officers in the development of risk-based performance standards aimed at reducing the risk of exposure to consumers. |
| format | Article |
| id | doaj-art-5d30acf07d484bf6afeebf5cbe8dc5d0 |
| institution | Kabale University |
| issn | 2674-1164 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
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| series | Poultry |
| spelling | doaj-art-5d30acf07d484bf6afeebf5cbe8dc5d02024-12-27T14:48:14ZengMDPI AGPoultry2674-11642024-09-013433034510.3390/poultry3040025Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in HondurasGabriela K. Betancourt-Barszcz0Sabrina E. Blandon1David A. Vargas2Rossy Bueno López3Daniela R. Chavez-Velado4Angelica Sánchez5Valeria Larios6Nadira J. Espinoza-Rock7Mirian Bueno-Almendarez8Marcos X. Sanchez-Plata9International Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAHonduran National Service of Agri-Food Safety and Health, SENASA, Tegucigalpa 11101, HondurasInternational Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USAPoultry bio-mapping helps identify microbial contamination and process optimization opportunities such as sanitary dressing procedures, equipment adjustments, cross-contamination controls, and chemical intervention applications in commercial poultry processing operations. This study focuses on the development of a preliminary microbiological baseline of microbial indicators, including total viable counts (TVC), Enterobacteriaceae (EB), and the quantification and detection of pathogens such as <i>Salmonella</i> and <i>Campylobacter</i> spp. in four commercial broiler processing facilities in Honduras. Whole chicken and wing rinses were collected from four poultry processing plants at different locations: live receiving, rehanger, post-evisceration, post-chiller, and wings. The MicroSnap<sup>®</sup> system was used for the enumeration of microbial indicators, the BAX<sup>®</sup>-System-SalQuant<sup>®</sup> was used for the quantification of <i>Salmonella</i>, and the BAX<sup>®</sup>-System-CampyQuant™ was used for <i>Campylobacter</i> spp. Negative samples after enumeration were tested with BAX<sup>®</sup>-System <i>Salmonella</i> and BAX<sup>®</sup>-System <i>Campylobacter</i> for prevalence analysis, respectively. The TVC and EB counts were continuously reduced from the live receiving to the post-chiller location, presenting a statistically significant increase (<i>p</i> < 0.01) at the wings location. The <i>Salmonella</i> counts were significantly different between stages (<i>p</i> < 0.01). The prevalence of <i>Salmonella</i> was highest in the live receiving stage, with 92.50%, while that at the post-chiller stage was the lowest, at 15.38%. <i>Campylobacter</i> spp. counts were lower than that at the other stages at wings 1.61 Log CFU/sample; however, <i>Campylobacter</i> spp.’s prevalence was higher than 62.5% in all stages. Microbial bio-mapping using novel technologies suitable for mobile applications was conducted in this study to establish statistical process control parameters for microorganisms. A nationwide microbial baseline for commercial broiler processing facilities in Honduras was developed. In-country data serve as a benchmark for continuous improvement at each facility evaluated and can assist regulatory officers in the development of risk-based performance standards aimed at reducing the risk of exposure to consumers.https://www.mdpi.com/2674-1164/3/4/25poultry bio-mappingmicrobial baselinetotal viable countsEnterobacteriaceae counts<i>Salmonella</i> enumeration<i>Campylobacter</i> spp. enumeration |
| spellingShingle | Gabriela K. Betancourt-Barszcz Sabrina E. Blandon David A. Vargas Rossy Bueno López Daniela R. Chavez-Velado Angelica Sánchez Valeria Larios Nadira J. Espinoza-Rock Mirian Bueno-Almendarez Marcos X. Sanchez-Plata Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in Honduras Poultry poultry bio-mapping microbial baseline total viable counts Enterobacteriaceae counts <i>Salmonella</i> enumeration <i>Campylobacter</i> spp. enumeration |
| title | Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in Honduras |
| title_full | Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in Honduras |
| title_fullStr | Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in Honduras |
| title_full_unstemmed | Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in Honduras |
| title_short | Use of a Mobile Methodology for the Bio-Mapping of Microbial Indicators and RT-PCR-Based Pathogen Quantification in Commercial Broiler Processing Facilities in Honduras |
| title_sort | use of a mobile methodology for the bio mapping of microbial indicators and rt pcr based pathogen quantification in commercial broiler processing facilities in honduras |
| topic | poultry bio-mapping microbial baseline total viable counts Enterobacteriaceae counts <i>Salmonella</i> enumeration <i>Campylobacter</i> spp. enumeration |
| url | https://www.mdpi.com/2674-1164/3/4/25 |
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