Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity
Abstract Perfluorooctane sulfonate (PFOS) is a widely used chemical in industrial production. It can be introduced into the environment through multiple pathways and exhibits resistance to degradation. Recent research has demonstrated a significant correlation between its exposure levels in the huma...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84732-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544744019165184 |
|---|---|
| author | Rui Ren Xinyue Zhou Tianyu Jia Bin Wang Ahui Liu Min Gao Ji Song Liyan Wang Yuanxue Jing Liulin Yu Haofei Shen Xuehong Zhang |
| author_facet | Rui Ren Xinyue Zhou Tianyu Jia Bin Wang Ahui Liu Min Gao Ji Song Liyan Wang Yuanxue Jing Liulin Yu Haofei Shen Xuehong Zhang |
| author_sort | Rui Ren |
| collection | DOAJ |
| description | Abstract Perfluorooctane sulfonate (PFOS) is a widely used chemical in industrial production. It can be introduced into the environment through multiple pathways and exhibits resistance to degradation. Recent research has demonstrated a significant correlation between its exposure levels in the human body and the incidence of various diseases. The expression of genes related to endometrial receptivity and the differentiation of human endometrial stromal cells (hESCs) were assessed in this study concerning PFOS. In this study, we investigated the effect of PFOS exposure on endometrial tolerance by cell and animal experiments. The activity against endometrial mesenchymal cells was significantly reduced by PFOS intervention, and the apoptosis flow assay results showed that PFOS significantly promoted cell death in a concentration-dependent manner. Transmission electron microscopy results revealed mitochondrial damage in the PFOS-intervened group, and WB results showed that the expression levels of endometrial tolerance-related proteins Homeobox A10 (HOXA10) and integrin beta3 (ITGB3) were decreased, and the expression level of Forkhead box O1 (FOXO1) protein was increased. Animal studies have shown that PFOS exposure can change uterine morphology, cause obvious damage to pinopodes morphology, change the estrous cycle of mice, and affect endometrial receptivity In the present study, we found that PFOS may synergistically affect the viability of endometrial mesenchymal stromal cells through accumulation in vivo, and that PFOS may contribute to the failure of embryo implantation by affecting mitochondrial function and consequently endometrial permissive sites. |
| format | Article |
| id | doaj-art-5d298195732849709917b2b28aec9246 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5d298195732849709917b2b28aec92462025-01-12T12:20:13ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-024-84732-2Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivityRui Ren0Xinyue Zhou1Tianyu Jia2Bin Wang3Ahui Liu4Min Gao5Ji Song6Liyan Wang7Yuanxue Jing8Liulin Yu9Haofei Shen10Xuehong Zhang11The First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Hospital of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityThe First Hospital of Lanzhou UniversityThe First Hospital of Lanzhou UniversityThe First Hospital of Lanzhou UniversityThe First Hospital of Lanzhou UniversityThe First Hospital of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityAbstract Perfluorooctane sulfonate (PFOS) is a widely used chemical in industrial production. It can be introduced into the environment through multiple pathways and exhibits resistance to degradation. Recent research has demonstrated a significant correlation between its exposure levels in the human body and the incidence of various diseases. The expression of genes related to endometrial receptivity and the differentiation of human endometrial stromal cells (hESCs) were assessed in this study concerning PFOS. In this study, we investigated the effect of PFOS exposure on endometrial tolerance by cell and animal experiments. The activity against endometrial mesenchymal cells was significantly reduced by PFOS intervention, and the apoptosis flow assay results showed that PFOS significantly promoted cell death in a concentration-dependent manner. Transmission electron microscopy results revealed mitochondrial damage in the PFOS-intervened group, and WB results showed that the expression levels of endometrial tolerance-related proteins Homeobox A10 (HOXA10) and integrin beta3 (ITGB3) were decreased, and the expression level of Forkhead box O1 (FOXO1) protein was increased. Animal studies have shown that PFOS exposure can change uterine morphology, cause obvious damage to pinopodes morphology, change the estrous cycle of mice, and affect endometrial receptivity In the present study, we found that PFOS may synergistically affect the viability of endometrial mesenchymal stromal cells through accumulation in vivo, and that PFOS may contribute to the failure of embryo implantation by affecting mitochondrial function and consequently endometrial permissive sites.https://doi.org/10.1038/s41598-024-84732-2Perfluorooctane sulfonate (PFOS)Endometrial stromal cellsEndometrial receptivityPinopodes morphology |
| spellingShingle | Rui Ren Xinyue Zhou Tianyu Jia Bin Wang Ahui Liu Min Gao Ji Song Liyan Wang Yuanxue Jing Liulin Yu Haofei Shen Xuehong Zhang Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity Scientific Reports Perfluorooctane sulfonate (PFOS) Endometrial stromal cells Endometrial receptivity Pinopodes morphology |
| title | Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity |
| title_full | Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity |
| title_fullStr | Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity |
| title_full_unstemmed | Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity |
| title_short | Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity |
| title_sort | developmental exposure to perfluorooctane sulfonate pfos impairs the endometrial receptivity |
| topic | Perfluorooctane sulfonate (PFOS) Endometrial stromal cells Endometrial receptivity Pinopodes morphology |
| url | https://doi.org/10.1038/s41598-024-84732-2 |
| work_keys_str_mv | AT ruiren developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT xinyuezhou developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT tianyujia developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT binwang developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT ahuiliu developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT mingao developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT jisong developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT liyanwang developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT yuanxuejing developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT liulinyu developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT haofeishen developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity AT xuehongzhang developmentalexposuretoperfluorooctanesulfonatepfosimpairstheendometrialreceptivity |