Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila Vinasse
Precise control of operational parameters in anaerobic digestion reactors is crucial to avoid imbalances that could affect biomethane production and alterations in the microbiota. Restoring the methanogenic microbiota after a failure is essential for recovering methane production, yet no published s...
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MDPI AG
2024-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/11/557 |
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| author | Adriana Serrano-Meza Iván Moreno-Andrade Edson B. Estrada-Arriaga Sergio A. Díaz-Barajas Liliana García-Sánchez Marco A. Garzón-Zúñiga |
| author_facet | Adriana Serrano-Meza Iván Moreno-Andrade Edson B. Estrada-Arriaga Sergio A. Díaz-Barajas Liliana García-Sánchez Marco A. Garzón-Zúñiga |
| author_sort | Adriana Serrano-Meza |
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| description | Precise control of operational parameters in anaerobic digestion reactors is crucial to avoid imbalances that could affect biomethane production and alterations in the microbiota. Restoring the methanogenic microbiota after a failure is essential for recovering methane production, yet no published strategies exist for this recovery. In this study, we restored the methanogenic microbiota in an anaerobic SBR reactor that operates with both biofilm and suspended biomass simultaneously, aiming to treat tequila vinasses. Four strategies were evaluated for restoring the methanogenic microbiota: reducing the initial vinasse concentration, increasing the reaction time (RT), adjusting the carbon/nitrogen (C/N) ratio, and progressively increasing the initial vinasse concentration. Among these, adjusting the C/N ratio emerged as a critical parameter for restoring organic matter removal efficiency and reestablishing methanogenic microbiota. The operational conditions under which the methanogenic activity and microbiota were restored were as follows: Operating the A-SBR with an initial vinasse concentration of 60%, an RT of 168 h, a pH of 6.9 ± 0.2, a temperature of 35 ± 2 °C, and a C/N ratio adjusted to 100/1.9 resulted in stable COD removal efficiency of 93 ± 3% over a year and a high percentage of methanogenic microorganisms in both the suspended microbiota (69%) and biofilm (52%). The normalized methane production (0.332 NL CH<sub>4</sub>/g CODr) approached the theoretical maximum value (0.35 L CH<sub>4</sub>/g CODr) after restoring the population and methanogenic activity within the reactor. |
| format | Article |
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| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-5ce4e159e9064c60a72dc2dfd9332bc02024-11-26T18:03:28ZengMDPI AGFermentation2311-56372024-10-01101155710.3390/fermentation10110557Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila VinasseAdriana Serrano-Meza0Iván Moreno-Andrade1Edson B. Estrada-Arriaga2Sergio A. Díaz-Barajas3Liliana García-Sánchez4Marco A. Garzón-Zúñiga5Laboratorio de Evaluación, Desarrollo e Innovación de Tecnología del Agua, CIIDIR-Durango, Instituto Politécnico Nacional, Sigma 119, 20 de Noviembre II, Durango 34220, MexicoLaboratory of Research on Advanced Processes for Water Treatment, Juriquilla Academic Unit, Institute of Engineering, National Autonomous University of Mexico, Blvd. Juriquilla 3001, Querétaro 76230, MexicoSubcoordinación de Sistemas de Saneamiento y Reutilización de Aguas Residuales, Instituto Mexicano de Tecnología del Agua, Paseo Cuauhnáhuac 8532, Jiutepec 62550, MexicoLaboratorio de Evaluación, Desarrollo e Innovación de Tecnología del Agua, CIIDIR-Durango, Instituto Politécnico Nacional, Sigma 119, 20 de Noviembre II, Durango 34220, MexicoSubcoordinación de Sistemas de Saneamiento y Reutilización de Aguas Residuales, Instituto Mexicano de Tecnología del Agua, Paseo Cuauhnáhuac 8532, Jiutepec 62550, MexicoLaboratorio de Evaluación, Desarrollo e Innovación de Tecnología del Agua, CIIDIR-Durango, Instituto Politécnico Nacional, Sigma 119, 20 de Noviembre II, Durango 34220, MexicoPrecise control of operational parameters in anaerobic digestion reactors is crucial to avoid imbalances that could affect biomethane production and alterations in the microbiota. Restoring the methanogenic microbiota after a failure is essential for recovering methane production, yet no published strategies exist for this recovery. In this study, we restored the methanogenic microbiota in an anaerobic SBR reactor that operates with both biofilm and suspended biomass simultaneously, aiming to treat tequila vinasses. Four strategies were evaluated for restoring the methanogenic microbiota: reducing the initial vinasse concentration, increasing the reaction time (RT), adjusting the carbon/nitrogen (C/N) ratio, and progressively increasing the initial vinasse concentration. Among these, adjusting the C/N ratio emerged as a critical parameter for restoring organic matter removal efficiency and reestablishing methanogenic microbiota. The operational conditions under which the methanogenic activity and microbiota were restored were as follows: Operating the A-SBR with an initial vinasse concentration of 60%, an RT of 168 h, a pH of 6.9 ± 0.2, a temperature of 35 ± 2 °C, and a C/N ratio adjusted to 100/1.9 resulted in stable COD removal efficiency of 93 ± 3% over a year and a high percentage of methanogenic microorganisms in both the suspended microbiota (69%) and biofilm (52%). The normalized methane production (0.332 NL CH<sub>4</sub>/g CODr) approached the theoretical maximum value (0.35 L CH<sub>4</sub>/g CODr) after restoring the population and methanogenic activity within the reactor.https://www.mdpi.com/2311-5637/10/11/557bioenergybiogasbiomethanemethanogenesisanaerobic digestionsuspended microbiota |
| spellingShingle | Adriana Serrano-Meza Iván Moreno-Andrade Edson B. Estrada-Arriaga Sergio A. Díaz-Barajas Liliana García-Sánchez Marco A. Garzón-Zúñiga Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila Vinasse Fermentation bioenergy biogas biomethane methanogenesis anaerobic digestion suspended microbiota |
| title | Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila Vinasse |
| title_full | Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila Vinasse |
| title_fullStr | Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila Vinasse |
| title_full_unstemmed | Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila Vinasse |
| title_short | Biomethane Production and Methanogenic Microbiota Restoration After a pH Failure in an Anaerobic Sequencing Batch Reactor (A-SBR) Treating Tequila Vinasse |
| title_sort | biomethane production and methanogenic microbiota restoration after a ph failure in an anaerobic sequencing batch reactor a sbr treating tequila vinasse |
| topic | bioenergy biogas biomethane methanogenesis anaerobic digestion suspended microbiota |
| url | https://www.mdpi.com/2311-5637/10/11/557 |
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