New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder
Introduction. High-quality dairy products depend on the properties of raw materials. Milk is the basic raw material for all dairy products. Its quality is subject to requirements of biological safety, and its physicochemical and technological properties comply with strict indicators. ϰ-casein gene i...
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Kemerovo State University
2020-10-01
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| Series: | Техника и технология пищевых производств |
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| author | Gilmanov Khamid Kh. Semipyatnyi Vladislav K. Bigaeva Alana V. Vafin Ramil R. Turovskaya Svetlana N. |
| author_facet | Gilmanov Khamid Kh. Semipyatnyi Vladislav K. Bigaeva Alana V. Vafin Ramil R. Turovskaya Svetlana N. |
| author_sort | Gilmanov Khamid Kh. |
| collection | DOAJ |
| description | Introduction. High-quality dairy products depend on the properties of raw materials. Milk is the basic raw material for all dairy products. Its quality is subject to requirements of biological safety, and its physicochemical and technological properties comply with strict indicators. ϰ-casein gene is a basic milk protein. Its allelic variants affect milk productivity, as well as its composition and technological properties, e.g. cheeseability and heat resistance. The research objective was to develop a new method for determining the ratio of the relative proportions of selection-significant kappa-casein gene alleles in bulk cow’s milk powder by means of molecular genetics and bioinformation system. The method can help to test raw materials for further processing rationality.
Study objects and methods. The research included a genetic analysis of samples of bulk whole milk powder, bulk powdered skimmed milk, and bulk raw milk. The developed method involved DNA extraction, combined PCR-RFLP technique, electrophoretic results, and analysis detection of information data using new mathematical algorithms and software.
Results and discussion. The ratio of the relative proportions of the kappa-casein gene alleles in milk powder was determined using primer sets JK5 and JK3, as well as restriction endonuclease HinfI for PCR-RFLP analysis. The experiment showed satisfactory reproducibility and interpretation of the obtained data. The program provided a ratio calculation of the kappa-casein gene alleles relative proportions in the studied milk powder samples, expressed as a percentage of the A allele proportion with additional indication of the absolute and relative errors. It also placed the information block of the generated numerical indicators into the percentage scale.
Conclusion. The developed methodology was implemented by a set of laboratory procedures. The new specialized program «Calculation of the relative proportions ratio of ϰ-casein alleles in bulk milk» is on open access. It provides a correct and prompt data interpretation, generated during the analysis of dry milk raw materials of bulk origin. |
| format | Article |
| id | doaj-art-5c8c6bffadbd48749a05b46dcbeaf0c9 |
| institution | Kabale University |
| issn | 2074-9414 2313-1748 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj-art-5c8c6bffadbd48749a05b46dcbeaf0c92024-12-02T00:03:51ZengKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482020-10-0150352553510.21603/2074-9414-2020-3-525-535New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powderGilmanov Khamid Kh. 0https://orcid.org/0000-0001-7053-6925Semipyatnyi Vladislav K. 1https://orcid.org/0000-0003-1241-0026Bigaeva Alana V. 2https://orcid.org/0000-0001-8400-2465Vafin Ramil R. 3https://orcid.org/0000-0003-0914-0053Turovskaya Svetlana N. 4https://orcid.org/0000-0002-5875-9875All-Russian Dairy Research InstituteAll-Russian Dairy Research InstituteAll-Russian Dairy Research InstituteAll-Russian Dairy Research InstituteAll-Russian Dairy Research InstituteIntroduction. High-quality dairy products depend on the properties of raw materials. Milk is the basic raw material for all dairy products. Its quality is subject to requirements of biological safety, and its physicochemical and technological properties comply with strict indicators. ϰ-casein gene is a basic milk protein. Its allelic variants affect milk productivity, as well as its composition and technological properties, e.g. cheeseability and heat resistance. The research objective was to develop a new method for determining the ratio of the relative proportions of selection-significant kappa-casein gene alleles in bulk cow’s milk powder by means of molecular genetics and bioinformation system. The method can help to test raw materials for further processing rationality. Study objects and methods. The research included a genetic analysis of samples of bulk whole milk powder, bulk powdered skimmed milk, and bulk raw milk. The developed method involved DNA extraction, combined PCR-RFLP technique, electrophoretic results, and analysis detection of information data using new mathematical algorithms and software. Results and discussion. The ratio of the relative proportions of the kappa-casein gene alleles in milk powder was determined using primer sets JK5 and JK3, as well as restriction endonuclease HinfI for PCR-RFLP analysis. The experiment showed satisfactory reproducibility and interpretation of the obtained data. The program provided a ratio calculation of the kappa-casein gene alleles relative proportions in the studied milk powder samples, expressed as a percentage of the A allele proportion with additional indication of the absolute and relative errors. It also placed the information block of the generated numerical indicators into the percentage scale. Conclusion. The developed methodology was implemented by a set of laboratory procedures. The new specialized program «Calculation of the relative proportions ratio of ϰ-casein alleles in bulk milk» is on open access. It provides a correct and prompt data interpretation, generated during the analysis of dry milk raw materials of bulk origin.milk productscaseinheat resistancecheese suitabilityallelecsn3pcrrflpalgorithms |
| spellingShingle | Gilmanov Khamid Kh. Semipyatnyi Vladislav K. Bigaeva Alana V. Vafin Ramil R. Turovskaya Svetlana N. New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder Техника и технология пищевых производств milk products casein heat resistance cheese suitability allele csn3 pcr rflp algorithms |
| title | New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder |
| title_full | New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder |
| title_fullStr | New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder |
| title_full_unstemmed | New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder |
| title_short | New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder |
| title_sort | new determination method for the ratio of the relative proportions of ϰ casein alleles in milk powder |
| topic | milk products casein heat resistance cheese suitability allele csn3 pcr rflp algorithms |
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