Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral side
Microbubble (MB)-assisted ultrasound (US) is an innovative modality for the non-invasive, targeted, and efficient delivery of therapeutic molecules into the brain. Previously, we reported the first metabolomic signature of blood–brain barrier opening (BBBO) induced by MB-assisted US. In the present...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2024.1383963/full |
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| author | Antoine Presset Sylvie Bodard Antoine Lefèvre Antoine Lefèvre Anaïs Millet Edward Oujagir Camille Dupuy Camille Dupuy Tarik Iazourène Ayache Bouakaz Patrick Emond Patrick Emond Patrick Emond Jean-Michel Escoffre Lydie Nadal-Desbarats Lydie Nadal-Desbarats |
| author_facet | Antoine Presset Sylvie Bodard Antoine Lefèvre Antoine Lefèvre Anaïs Millet Edward Oujagir Camille Dupuy Camille Dupuy Tarik Iazourène Ayache Bouakaz Patrick Emond Patrick Emond Patrick Emond Jean-Michel Escoffre Lydie Nadal-Desbarats Lydie Nadal-Desbarats |
| author_sort | Antoine Presset |
| collection | DOAJ |
| description | Microbubble (MB)-assisted ultrasound (US) is an innovative modality for the non-invasive, targeted, and efficient delivery of therapeutic molecules into the brain. Previously, we reported the first metabolomic signature of blood–brain barrier opening (BBBO) induced by MB-assisted US. In the present study, the neurometabolic consequences of acoustically-mediated BBBO on cerebral tissue were investigated using multimodal metabolomics approaches. Sinusoid US waves (1 MHz, peak negative pressure 0.6 MPa, burst length 10 ms, total treatment time 30 s, MB bolus dose 0.7 × 105 MBs/g) were applied on the rats’ right striatum (ipsilateral side). Brain was collected and both striata were then dissected 3 h, 2 days, and 1 week after BBBO. After tissue preparation, the samples were analyzed using nuclear magnetic resonance spectrometry (NMRS) and high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Our findings showed a slight disruption of metabolic pathways in contralateral striata of animals. Analyses of metabolic pathways indicated changes in amino acid metabolisms. In addition, tryptophan derivate dosages revealed the perturbation of a central metabolite of the kynurenine pathway (i.e., 3-hydroxy-kynurenine). In conclusion, the acoustically-mediated BBBO of the ipsilateral cerebral hemisphere induced significant change in metabolism of contralateral one. |
| format | Article |
| id | doaj-art-5c7f09dbd46b43ff9c3b34697ff9cf0c |
| institution | Kabale University |
| issn | 1662-5099 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Neuroscience |
| spelling | doaj-art-5c7f09dbd46b43ff9c3b34697ff9cf0c2024-11-20T06:27:52ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992024-11-011710.3389/fnmol.2024.13839631383963Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral sideAntoine Presset0Sylvie Bodard1Antoine Lefèvre2Antoine Lefèvre3Anaïs Millet4Edward Oujagir5Camille Dupuy6Camille Dupuy7Tarik Iazourène8Ayache Bouakaz9Patrick Emond10Patrick Emond11Patrick Emond12Jean-Michel Escoffre13Lydie Nadal-Desbarats14Lydie Nadal-Desbarats15UMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceDépartement Analyses Chimique et Métabolomique, PST Analyses des Systèmes Biologiques, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceDépartement Analyses Chimique et Métabolomique, PST Analyses des Systèmes Biologiques, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceDépartement Analyses Chimique et Métabolomique, PST Analyses des Systèmes Biologiques, Université de Tours, Tours, FranceCHRU Tours, Serv Med Nucl in Vitro, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceUMR 1253, iBrain, Inserm, Université de Tours, Tours, FranceDépartement Analyses Chimique et Métabolomique, PST Analyses des Systèmes Biologiques, Université de Tours, Tours, FranceMicrobubble (MB)-assisted ultrasound (US) is an innovative modality for the non-invasive, targeted, and efficient delivery of therapeutic molecules into the brain. Previously, we reported the first metabolomic signature of blood–brain barrier opening (BBBO) induced by MB-assisted US. In the present study, the neurometabolic consequences of acoustically-mediated BBBO on cerebral tissue were investigated using multimodal metabolomics approaches. Sinusoid US waves (1 MHz, peak negative pressure 0.6 MPa, burst length 10 ms, total treatment time 30 s, MB bolus dose 0.7 × 105 MBs/g) were applied on the rats’ right striatum (ipsilateral side). Brain was collected and both striata were then dissected 3 h, 2 days, and 1 week after BBBO. After tissue preparation, the samples were analyzed using nuclear magnetic resonance spectrometry (NMRS) and high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Our findings showed a slight disruption of metabolic pathways in contralateral striata of animals. Analyses of metabolic pathways indicated changes in amino acid metabolisms. In addition, tryptophan derivate dosages revealed the perturbation of a central metabolite of the kynurenine pathway (i.e., 3-hydroxy-kynurenine). In conclusion, the acoustically-mediated BBBO of the ipsilateral cerebral hemisphere induced significant change in metabolism of contralateral one.https://www.frontiersin.org/articles/10.3389/fnmol.2024.1383963/fullblood–brain barrier openingmetabolomicsbrain’s contralateral sideamino acid metabolismscompensatory mechanismultrasound |
| spellingShingle | Antoine Presset Sylvie Bodard Antoine Lefèvre Antoine Lefèvre Anaïs Millet Edward Oujagir Camille Dupuy Camille Dupuy Tarik Iazourène Ayache Bouakaz Patrick Emond Patrick Emond Patrick Emond Jean-Michel Escoffre Lydie Nadal-Desbarats Lydie Nadal-Desbarats Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral side Frontiers in Molecular Neuroscience blood–brain barrier opening metabolomics brain’s contralateral side amino acid metabolisms compensatory mechanism ultrasound |
| title | Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral side |
| title_full | Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral side |
| title_fullStr | Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral side |
| title_full_unstemmed | Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral side |
| title_short | Metabolomic profile of cerebral tissue after acoustically-mediated blood-brain barrier opening in a healthy rat model: a focus on the contralateral side |
| title_sort | metabolomic profile of cerebral tissue after acoustically mediated blood brain barrier opening in a healthy rat model a focus on the contralateral side |
| topic | blood–brain barrier opening metabolomics brain’s contralateral side amino acid metabolisms compensatory mechanism ultrasound |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2024.1383963/full |
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