Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo
Although apoptosis can be readily assessed in vitro with a variety of techniques, the detection of apoptosis in the in vivo setting poses a much more difficult proposition. Apoptosis in an organism is followed almost inevitably by rapid clearance of dying cells via phagocytosis, thus limiting the ab...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00017 |
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| author | Mukesh K. Nyati Felix Y. Feng Vinayak D. Kanade Rabindranath Nayak |
| author_facet | Mukesh K. Nyati Felix Y. Feng Vinayak D. Kanade Rabindranath Nayak |
| author_sort | Mukesh K. Nyati |
| collection | DOAJ |
| description | Although apoptosis can be readily assessed in vitro with a variety of techniques, the detection of apoptosis in the in vivo setting poses a much more difficult proposition. Apoptosis in an organism is followed almost inevitably by rapid clearance of dying cells via phagocytosis, thus limiting the ability to analyze apoptosis in vivo using classical techniques. To address this issue, we developed a method to enhance in vivo apoptosis detection using pretreatment with chloroquine, an inhibitor of macrophage activity, in Swiss albino mice. This technique resulted in a significant increase in the accumulation of apoptotic cells induced by 5-fluorouracil, as detected by propidium iodide staining in solid and ascitic forms of Ehrlich ascitic tumors and in bone marrow cells. We further validated our technique using DNA fragmentation and endonuclease assays. Our results demonstrated that chloroquine pretreatment can significantly enhance accumulation of apoptotic cells in organisms, and we envision combining this method with modern imaging techniques to optimize in vivo detection of apoptosis. |
| format | Article |
| id | doaj-art-59a5c8eb8e9342228fa009747b23dd1d |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2006-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-59a5c8eb8e9342228fa009747b23dd1d2025-01-03T00:12:14ZengSAGE PublishingMolecular Imaging1536-01212006-07-01510.2310/7290.2006.0001710.2310_7290.2006.00017Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In VivoMukesh K. Nyati0Felix Y. Feng1Vinayak D. Kanade2Rabindranath Nayak3Indian Institute of Science, IndiaUniversity of MichiganIndian Institute of Science, IndiaIndian Institute of Science, IndiaAlthough apoptosis can be readily assessed in vitro with a variety of techniques, the detection of apoptosis in the in vivo setting poses a much more difficult proposition. Apoptosis in an organism is followed almost inevitably by rapid clearance of dying cells via phagocytosis, thus limiting the ability to analyze apoptosis in vivo using classical techniques. To address this issue, we developed a method to enhance in vivo apoptosis detection using pretreatment with chloroquine, an inhibitor of macrophage activity, in Swiss albino mice. This technique resulted in a significant increase in the accumulation of apoptotic cells induced by 5-fluorouracil, as detected by propidium iodide staining in solid and ascitic forms of Ehrlich ascitic tumors and in bone marrow cells. We further validated our technique using DNA fragmentation and endonuclease assays. Our results demonstrated that chloroquine pretreatment can significantly enhance accumulation of apoptotic cells in organisms, and we envision combining this method with modern imaging techniques to optimize in vivo detection of apoptosis.https://doi.org/10.2310/7290.2006.00017 |
| spellingShingle | Mukesh K. Nyati Felix Y. Feng Vinayak D. Kanade Rabindranath Nayak Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo Molecular Imaging |
| title | Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo |
| title_full | Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo |
| title_fullStr | Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo |
| title_full_unstemmed | Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo |
| title_short | Chloroquine Treatment Increases Detection of 5-Fluorouracil-Induced Apoptosis In Vivo |
| title_sort | chloroquine treatment increases detection of 5 fluorouracil induced apoptosis in vivo |
| url | https://doi.org/10.2310/7290.2006.00017 |
| work_keys_str_mv | AT mukeshknyati chloroquinetreatmentincreasesdetectionof5fluorouracilinducedapoptosisinvivo AT felixyfeng chloroquinetreatmentincreasesdetectionof5fluorouracilinducedapoptosisinvivo AT vinayakdkanade chloroquinetreatmentincreasesdetectionof5fluorouracilinducedapoptosisinvivo AT rabindranathnayak chloroquinetreatmentincreasesdetectionof5fluorouracilinducedapoptosisinvivo |