Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier model
Abstract Repeated muscle micro-trauma may cause severe muscle damage. Diffusion tensor imaging (DTI) exhibits sensitivity to microstructural changes in skeletal muscle. We hypothesize that longer diffusion times enhance sensitivity to micro-trauma and that membrane permeability increases with micro-...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-83644-5 |
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| author | Susanne S. Rauh Donnie Cameron Oliver J. Gurney-Champion Frank Smithuis Mario Maas Martijn Froeling Hermien E. Kan Aart J. Nederveen Gustav J. Strijkers Melissa T. Hooijmans |
| author_facet | Susanne S. Rauh Donnie Cameron Oliver J. Gurney-Champion Frank Smithuis Mario Maas Martijn Froeling Hermien E. Kan Aart J. Nederveen Gustav J. Strijkers Melissa T. Hooijmans |
| author_sort | Susanne S. Rauh |
| collection | DOAJ |
| description | Abstract Repeated muscle micro-trauma may cause severe muscle damage. Diffusion tensor imaging (DTI) exhibits sensitivity to microstructural changes in skeletal muscle. We hypothesize that longer diffusion times enhance sensitivity to micro-trauma and that membrane permeability increases with micro-trauma. We obtained DTI scans of the thighs in ten male runners 1 week before (TP-0), 24–48 h after (TP-1), and 2 weeks after (TP-2) they completed a marathon. Diffusion times were 28.1, 116.7, and 316.7 ms. The random permeable barrier model (RPBM) was fitted to the radial diffusivities to obtain estimates for fiber diameter and membrane permeability. Hamstring and quadriceps muscles were manually segmented. A linear mixed model assessed variations across time points and diffusion times within the DTI and RPBM parameters and assessed sensitivity to micro-trauma by comparing %-changes in DTI parameters at TP-1 and TP-2 to TP-0. All DTI parameters except FA significantly changed between TP-0 and TP-1, and between TP-1 and TP-2. The %-change did not differ between diffusion times. The permeability increased at TP-1 and TP-2 compared to TP-0. In conclusion, longer diffusion times did not improve sensitivity to micro-trauma. The increased permeability post-marathon underscores the potential of RPBM-derived parameters as a biomarker for micro-trauma and muscle injuries. |
| format | Article |
| id | doaj-art-58e831d999c54bc68ee02dd48421c38c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-58e831d999c54bc68ee02dd48421c38c2025-01-05T12:23:52ZengNature PortfolioScientific Reports2045-23222024-12-0114111210.1038/s41598-024-83644-5Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier modelSusanne S. Rauh0Donnie Cameron1Oliver J. Gurney-Champion2Frank Smithuis3Mario Maas4Martijn Froeling5Hermien E. Kan6Aart J. Nederveen7Gustav J. Strijkers8Melissa T. Hooijmans9Department of Biomedical Engineering and Physics, Amsterdam University Medical CenterC.J. Gorter MRI Center, Department of Radiology, Leiden University Medical CenterDepartment of Radiology and Nuclear Medicine, Amsterdam University Medical CenterDepartment of Radiology and Nuclear Medicine, Amsterdam University Medical CenterDepartment of Radiology and Nuclear Medicine, Amsterdam University Medical CenterDepartment of Radiology, Utrecht University Medical CenterC.J. Gorter MRI Center, Department of Radiology, Leiden University Medical CenterDepartment of Radiology and Nuclear Medicine, Amsterdam University Medical CenterDepartment of Biomedical Engineering and Physics, Amsterdam University Medical CenterAmsterdam Movement Sciences, SportsAbstract Repeated muscle micro-trauma may cause severe muscle damage. Diffusion tensor imaging (DTI) exhibits sensitivity to microstructural changes in skeletal muscle. We hypothesize that longer diffusion times enhance sensitivity to micro-trauma and that membrane permeability increases with micro-trauma. We obtained DTI scans of the thighs in ten male runners 1 week before (TP-0), 24–48 h after (TP-1), and 2 weeks after (TP-2) they completed a marathon. Diffusion times were 28.1, 116.7, and 316.7 ms. The random permeable barrier model (RPBM) was fitted to the radial diffusivities to obtain estimates for fiber diameter and membrane permeability. Hamstring and quadriceps muscles were manually segmented. A linear mixed model assessed variations across time points and diffusion times within the DTI and RPBM parameters and assessed sensitivity to micro-trauma by comparing %-changes in DTI parameters at TP-1 and TP-2 to TP-0. All DTI parameters except FA significantly changed between TP-0 and TP-1, and between TP-1 and TP-2. The %-change did not differ between diffusion times. The permeability increased at TP-1 and TP-2 compared to TP-0. In conclusion, longer diffusion times did not improve sensitivity to micro-trauma. The increased permeability post-marathon underscores the potential of RPBM-derived parameters as a biomarker for micro-trauma and muscle injuries.https://doi.org/10.1038/s41598-024-83644-5 |
| spellingShingle | Susanne S. Rauh Donnie Cameron Oliver J. Gurney-Champion Frank Smithuis Mario Maas Martijn Froeling Hermien E. Kan Aart J. Nederveen Gustav J. Strijkers Melissa T. Hooijmans Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier model Scientific Reports |
| title | Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier model |
| title_full | Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier model |
| title_fullStr | Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier model |
| title_full_unstemmed | Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier model |
| title_short | Investigating skeletal muscle micro-trauma with time-dependent diffusion and the random permeable barrier model |
| title_sort | investigating skeletal muscle micro trauma with time dependent diffusion and the random permeable barrier model |
| url | https://doi.org/10.1038/s41598-024-83644-5 |
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