Development of SNAP-Tag Based Nanobodies as Secondary Antibody Mimics for Indirect Immunofluorescence Assays

Development of SNAP-Tag Based Nanobodies as Secondary Antibody Mimics for Indirect Immunofluorescence Assays

The immunofluorescence assay is widely used for cellular biology and diagnosis applications. Such an antigen–antibody detection system enables the assessment and visualization of the expression and localization of target proteins. In the classical indirect immunofluorescence assay, secondary antibod...

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Main Authors: Wenjie Sheng, Chaoyu Zhang, T. M. Mohiuddin, Marwah Al-Rawe, Roland Schmitz, Marcus Niebert, Lutz Konrad, Steffen Wagner, Felix Zeppernick, Ivo Meinhold-Heerlein, Ahmad Fawzi Hussain
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Cells
Subjects:
immunofluorescence assays
fluorescence imaging
nanobody
SNAP-tag technology
secondary antibody
Online Access:https://www.mdpi.com/2073-4409/14/10/691
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