Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cells
Microplastics (MPs) accumulate in tissues, including kidney tissue, while Bisphenol A (BPA) is a plasticizer of particular concern. At present, the combined effects of MPs and BPA are unexplored in human renal cells. Therefore, we exposed a proximal tubular cell line (PTECs) to polyethylene (PE)-MPs...
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Elsevier
2024-10-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024154576 |
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| author | Daniela Verzola Noemi Rumeo Stefano Alberti Fabrizio Loiacono Sebastiano La Maestra Mario Passalacqua Cristina Artini Elisa Russo Enrico Verrina Andrea Angeletti Simona Matarese Nicoletta Mancianti Paolo Cravedi Micaela Gentile Francesca Viazzi Pasquale Esposito Edoardo La Porta |
| author_facet | Daniela Verzola Noemi Rumeo Stefano Alberti Fabrizio Loiacono Sebastiano La Maestra Mario Passalacqua Cristina Artini Elisa Russo Enrico Verrina Andrea Angeletti Simona Matarese Nicoletta Mancianti Paolo Cravedi Micaela Gentile Francesca Viazzi Pasquale Esposito Edoardo La Porta |
| author_sort | Daniela Verzola |
| collection | DOAJ |
| description | Microplastics (MPs) accumulate in tissues, including kidney tissue, while Bisphenol A (BPA) is a plasticizer of particular concern. At present, the combined effects of MPs and BPA are unexplored in human renal cells. Therefore, we exposed a proximal tubular cell line (PTECs) to polyethylene (PE)-MPs and BPA, both separately and in combination. When co-exposed, cells showed a significantly reduced cell viability (MTT test) and a pronounced pro-oxidant (MDA levels, NRF2 and NOX4 expression by Western blot) and pro-inflammatory response (IL1β, CCL/CCR2 and CCL/CCR5 mRNAs by RT-PCR), compared to those treated with a single compound. In addition, heat shock protein (HSP90), a chaperone involved in multiple cellular functions, was reduced (by Western Blot and immunocytochemistry), while aryl hydrocarbon receptor (AHR) expression, a transcription factor which binds environmental ligands, was increased (RT-PCR and immunofluorescence). Our research can contribute to the study of the nephrotoxic effects of pollutants and MPs and shed new light on the combined effects of BPA and PE-MPs. |
| format | Article |
| id | doaj-art-574d6acb37604437a1010c65c2276f29 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-574d6acb37604437a1010c65c2276f292024-11-12T05:20:39ZengElsevierHeliyon2405-84402024-10-011020e39426Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cellsDaniela Verzola0Noemi Rumeo1Stefano Alberti2Fabrizio Loiacono3Sebastiano La Maestra4Mario Passalacqua5Cristina Artini6Elisa Russo7Enrico Verrina8Andrea Angeletti9Simona Matarese10Nicoletta Mancianti11Paolo Cravedi12Micaela Gentile13Francesca Viazzi14Pasquale Esposito15Edoardo La Porta16Department of Internal Medicine and Medical Specialties (DIMI), University of Genoa, Genoa, ItalyLaboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, ItalyDCCI, Department of Chemistry and Industrial Chemistry, University of Genoa, ItalyU.O.C. Immunologia, IRCCS Ospedale Policlinico San Martino, Genoa, ItalyDepartment of Health Sciences, University of Genoa, Genoa, ItalySection Biochemistry, Department of Experimental Medicine (DIMES), University of Genoa, Genoa, ItalyDCCI, Department of Chemistry and Industrial Chemistry, University of Genoa, Italy; Institute of Condensed Matter Chemistry and Technologies for Energy, National Research Council, CNR-ICMATE, Genoa, ItalyDepartment of Internal Medicine and Medical Specialties (DIMI), University of Genoa, Genoa, Italy; Unit of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, ItalyUOC Nephrology IRCCS Istituto Giannina Gaslini, Genoa, Italy; UOSD Dialysis IRCCS Istituto Giannina Gaslini, Genoa, ItalyLaboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy; UOC Nephrology IRCCS Istituto Giannina Gaslini, Genoa, ItalyDepartment of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genoa, Genoa, ItalyDepartment of Emergency-Urgency and Transplantation, Nephrology, Dialysis and Transplantation Unit, University Hospital of Siena, Siena, ItalyDivision of Nephrology, Translational Transplant Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USADivision of Nephrology, Translational Transplant Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA; UO Nefrologia, Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, ItalyDepartment of Internal Medicine and Medical Specialties (DIMI), University of Genoa, Genoa, Italy; Unit of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, ItalyDepartment of Internal Medicine and Medical Specialties (DIMI), University of Genoa, Genoa, Italy; Unit of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino, Genoa, Italy; Corresponding author. Department of Internal Medicine and Medical Specialties (DIMI), University of Genoa, Genoa, Italy.UOC Nephrology IRCCS Istituto Giannina Gaslini, Genoa, Italy; UOSD Dialysis IRCCS Istituto Giannina Gaslini, Genoa, ItalyMicroplastics (MPs) accumulate in tissues, including kidney tissue, while Bisphenol A (BPA) is a plasticizer of particular concern. At present, the combined effects of MPs and BPA are unexplored in human renal cells. Therefore, we exposed a proximal tubular cell line (PTECs) to polyethylene (PE)-MPs and BPA, both separately and in combination. When co-exposed, cells showed a significantly reduced cell viability (MTT test) and a pronounced pro-oxidant (MDA levels, NRF2 and NOX4 expression by Western blot) and pro-inflammatory response (IL1β, CCL/CCR2 and CCL/CCR5 mRNAs by RT-PCR), compared to those treated with a single compound. In addition, heat shock protein (HSP90), a chaperone involved in multiple cellular functions, was reduced (by Western Blot and immunocytochemistry), while aryl hydrocarbon receptor (AHR) expression, a transcription factor which binds environmental ligands, was increased (RT-PCR and immunofluorescence). Our research can contribute to the study of the nephrotoxic effects of pollutants and MPs and shed new light on the combined effects of BPA and PE-MPs.http://www.sciencedirect.com/science/article/pii/S2405844024154576Polyethylene-microplasticsBisphenol AKidney tubular cellsCell damage |
| spellingShingle | Daniela Verzola Noemi Rumeo Stefano Alberti Fabrizio Loiacono Sebastiano La Maestra Mario Passalacqua Cristina Artini Elisa Russo Enrico Verrina Andrea Angeletti Simona Matarese Nicoletta Mancianti Paolo Cravedi Micaela Gentile Francesca Viazzi Pasquale Esposito Edoardo La Porta Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cells Heliyon Polyethylene-microplastics Bisphenol A Kidney tubular cells Cell damage |
| title | Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cells |
| title_full | Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cells |
| title_fullStr | Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cells |
| title_full_unstemmed | Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cells |
| title_short | Coexposure to microplastic and Bisphenol A exhacerbates damage to human kidney proximal tubular cells |
| title_sort | coexposure to microplastic and bisphenol a exhacerbates damage to human kidney proximal tubular cells |
| topic | Polyethylene-microplastics Bisphenol A Kidney tubular cells Cell damage |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024154576 |
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