Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegans
Objective: This study aimed to explore the mitigating effects of germinated foxtail millet bioactive peptides (GRBPs) on copper-induced oxidative damage in Caenorhabditis elegans (C. elegans) and its potential mechanisms. Methods: By randomly grouping, the wild-type N2 C. elegans were divided into a...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2025-01-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010368 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841550182629507072 |
|---|---|
| author | Huiqi ZHANG Xiao CHEN Xueling JIN Jingjing LI Juanli YUAN Jinyan GAO Hongbing CHEN Yong WU |
| author_facet | Huiqi ZHANG Xiao CHEN Xueling JIN Jingjing LI Juanli YUAN Jinyan GAO Hongbing CHEN Yong WU |
| author_sort | Huiqi ZHANG |
| collection | DOAJ |
| description | Objective: This study aimed to explore the mitigating effects of germinated foxtail millet bioactive peptides (GRBPs) on copper-induced oxidative damage in Caenorhabditis elegans (C. elegans) and its potential mechanisms. Methods: By randomly grouping, the wild-type N2 C. elegans were divided into a control group and GRBPs groups to examine the effects of GRBPs feeding on mobility and survival rate of C. elegans induced by copper. The impact of GRBPs on oxidative damage was assessed by measuring the levels of reactive oxygen species (ROS), glutathione (GSH), and the activities of catalase (CAT), and superoxide dismutase (SOD). Furthermore, the mechanism of action of GRBPs was explored through genomic and transcriptomic sequencing techniques (RNA-seq) and untargeted metabolomics analysis. Antioxidant-related genes were validated by reverse transcription and real-time quantitative PCR (qPCR). Results: GRBPs obviously improved the survival rate and mobility of C. elegans after copper treatment, and a concentration of 1 mg/mL of GRBPs extremely significantly reduced the levels of MDA (P<0.0001), increased the activities of CAT and SOD, and regulated the expression of specific genes (such as upregulation of ctl-1, ctl-2, and downregulation of sod-1, sod-2). At the genetic level, GRBPs mainly regulated the structural constituent of cuticle and the expression of genes related to cytochrome enzyme P450 in C. elegans. At the metabolic level, GRBPs mainly regulated the content of biosynthesis of plant secondary metabolites in C. elegans. Conclusions: This study demonstrates that germinated millet bioactive peptides can effectively alleviate copper-induced oxidative damage, possibly through enhancing the activity of antioxidant enzymes, reducing the expression of cuticle and cytochrome enzyme P450 related genes, and regulating the content of plant secondary metabolites. |
| format | Article |
| id | doaj-art-534b81a7130a4ae7ad5c62dc2729567b |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-534b81a7130a4ae7ad5c62dc2729567b2025-01-10T06:49:17ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-01-0146138439310.13386/j.issn1002-0306.20240103682024010368-1Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegansHuiqi ZHANG0Xiao CHEN1Xueling JIN2Jingjing LI3Juanli YUAN4Jinyan GAO5Hongbing CHEN6Yong WU7State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaThe First Affiliated Hospital of Nanchang University, Nanchang 330006, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, ChinaSino German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaObjective: This study aimed to explore the mitigating effects of germinated foxtail millet bioactive peptides (GRBPs) on copper-induced oxidative damage in Caenorhabditis elegans (C. elegans) and its potential mechanisms. Methods: By randomly grouping, the wild-type N2 C. elegans were divided into a control group and GRBPs groups to examine the effects of GRBPs feeding on mobility and survival rate of C. elegans induced by copper. The impact of GRBPs on oxidative damage was assessed by measuring the levels of reactive oxygen species (ROS), glutathione (GSH), and the activities of catalase (CAT), and superoxide dismutase (SOD). Furthermore, the mechanism of action of GRBPs was explored through genomic and transcriptomic sequencing techniques (RNA-seq) and untargeted metabolomics analysis. Antioxidant-related genes were validated by reverse transcription and real-time quantitative PCR (qPCR). Results: GRBPs obviously improved the survival rate and mobility of C. elegans after copper treatment, and a concentration of 1 mg/mL of GRBPs extremely significantly reduced the levels of MDA (P<0.0001), increased the activities of CAT and SOD, and regulated the expression of specific genes (such as upregulation of ctl-1, ctl-2, and downregulation of sod-1, sod-2). At the genetic level, GRBPs mainly regulated the structural constituent of cuticle and the expression of genes related to cytochrome enzyme P450 in C. elegans. At the metabolic level, GRBPs mainly regulated the content of biosynthesis of plant secondary metabolites in C. elegans. Conclusions: This study demonstrates that germinated millet bioactive peptides can effectively alleviate copper-induced oxidative damage, possibly through enhancing the activity of antioxidant enzymes, reducing the expression of cuticle and cytochrome enzyme P450 related genes, and regulating the content of plant secondary metabolites.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010368germinated foxtail millet bioactive peptidesc. eleganscopper inducedoxidative damage |
| spellingShingle | Huiqi ZHANG Xiao CHEN Xueling JIN Jingjing LI Juanli YUAN Jinyan GAO Hongbing CHEN Yong WU Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegans Shipin gongye ke-ji germinated foxtail millet bioactive peptides c. elegans copper induced oxidative damage |
| title | Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegans |
| title_full | Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegans |
| title_fullStr | Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegans |
| title_full_unstemmed | Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegans |
| title_short | Molecular Mechanism of Germinated Foxtail Millet Bioactive Peptides in Attenuating Copper-induced Oxidative Damage in Caenorhabditis elegans |
| title_sort | molecular mechanism of germinated foxtail millet bioactive peptides in attenuating copper induced oxidative damage in caenorhabditis elegans |
| topic | germinated foxtail millet bioactive peptides c. elegans copper induced oxidative damage |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024010368 |
| work_keys_str_mv | AT huiqizhang molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans AT xiaochen molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans AT xuelingjin molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans AT jingjingli molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans AT juanliyuan molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans AT jinyangao molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans AT hongbingchen molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans AT yongwu molecularmechanismofgerminatedfoxtailmilletbioactivepeptidesinattenuatingcopperinducedoxidativedamageincaenorhabditiselegans |