Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation
Human bone marrow mesenchymal stem cells (BM-MSCs) and cardiac progenitor/stem cells (CPCs) have been extensively studied as a potential therapeutic treatment for myocardial infarction (MI). Previous reports suggest that lower doses of CPCs are needed to improve cardiac function relative to their bo...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8872009 |
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| author | Imelda Ontoria-Oviedo Itziar Palacios Joaquín Panadero Belén Sánchez Francisco García-García Adolfo López-Cerdán Akaitz Dorronsoro Delia Castellano Luis Rodríguez-Borlado Antonio Bernad Pilar Sepúlveda |
| author_facet | Imelda Ontoria-Oviedo Itziar Palacios Joaquín Panadero Belén Sánchez Francisco García-García Adolfo López-Cerdán Akaitz Dorronsoro Delia Castellano Luis Rodríguez-Borlado Antonio Bernad Pilar Sepúlveda |
| author_sort | Imelda Ontoria-Oviedo |
| collection | DOAJ |
| description | Human bone marrow mesenchymal stem cells (BM-MSCs) and cardiac progenitor/stem cells (CPCs) have been extensively studied as a potential therapeutic treatment for myocardial infarction (MI). Previous reports suggest that lower doses of CPCs are needed to improve cardiac function relative to their bone marrow counterparts. Here, we confirmed this observations and investigated the surface protein expression profile that might explain this effect. Myocardial infarction was performed in nude rats by permanent ligation of the left coronary artery. Cardiac function and infarct size before and after cell transplantation were evaluated by echocardiography and morphometry, respectively. The CPC and BM-MSC receptome were analyzed by proteomic analysis of biotin-labeled surface proteins. Rats transplanted with CPCs showed a greater improvement in cardiac function after MI than those transplanted with BM-MSCs, and this was associated with a smaller infarct size. Analysis of the receptome of CPCs and BM-MSCs showed that gene ontology biological processes and KEGG pathways associated with adhesion mechanisms were upregulated in CPCs compared with BM-MSCs. Moreover, the membrane protein interactome in CPCs showed a strong relationship with biological processes related to cell adhesion whereas the BM-MSCs interactome was more related to immune regulation processes. We conclude that the stronger capacity of CPCs over BM-MSCs to engraft in the infarcted area is likely linked to a more pronounced cell adhesion expression program. |
| format | Article |
| id | doaj-art-532b673053834ff190ac91d94772259e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-532b673053834ff190ac91d94772259e2025-02-03T05:52:58ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88720098872009Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell TransplantationImelda Ontoria-Oviedo0Itziar Palacios1Joaquín Panadero2Belén Sánchez3Francisco García-García4Adolfo López-Cerdán5Akaitz Dorronsoro6Delia Castellano7Luis Rodríguez-Borlado8Antonio Bernad9Pilar Sepúlveda10Regenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, SpainCoretherapix, SLU, Tres Cantos, 28760 Madrid, SpainIGENOMIX S.L, 46980 Paterna, Valencia, SpainCoretherapix, SLU, Tres Cantos, 28760 Madrid, SpainBioinformatics and Biostatistics Unit, Centro de Investigación Príncipe Felipe, 46012 Valencia, SpainBioinformatics and Biostatistics Unit, Centro de Investigación Príncipe Felipe, 46012 Valencia, SpainRegenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, SpainRegenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, SpainCoretherapix, SLU, Tres Cantos, 28760 Madrid, SpainDepartment of Immunology and Oncology, Centro Nacional de Biotecnología (CNB-CSIC), 28048 Madrid, SpainRegenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, SpainHuman bone marrow mesenchymal stem cells (BM-MSCs) and cardiac progenitor/stem cells (CPCs) have been extensively studied as a potential therapeutic treatment for myocardial infarction (MI). Previous reports suggest that lower doses of CPCs are needed to improve cardiac function relative to their bone marrow counterparts. Here, we confirmed this observations and investigated the surface protein expression profile that might explain this effect. Myocardial infarction was performed in nude rats by permanent ligation of the left coronary artery. Cardiac function and infarct size before and after cell transplantation were evaluated by echocardiography and morphometry, respectively. The CPC and BM-MSC receptome were analyzed by proteomic analysis of biotin-labeled surface proteins. Rats transplanted with CPCs showed a greater improvement in cardiac function after MI than those transplanted with BM-MSCs, and this was associated with a smaller infarct size. Analysis of the receptome of CPCs and BM-MSCs showed that gene ontology biological processes and KEGG pathways associated with adhesion mechanisms were upregulated in CPCs compared with BM-MSCs. Moreover, the membrane protein interactome in CPCs showed a strong relationship with biological processes related to cell adhesion whereas the BM-MSCs interactome was more related to immune regulation processes. We conclude that the stronger capacity of CPCs over BM-MSCs to engraft in the infarcted area is likely linked to a more pronounced cell adhesion expression program.http://dx.doi.org/10.1155/2020/8872009 |
| spellingShingle | Imelda Ontoria-Oviedo Itziar Palacios Joaquín Panadero Belén Sánchez Francisco García-García Adolfo López-Cerdán Akaitz Dorronsoro Delia Castellano Luis Rodríguez-Borlado Antonio Bernad Pilar Sepúlveda Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation Stem Cells International |
| title | Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation |
| title_full | Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation |
| title_fullStr | Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation |
| title_full_unstemmed | Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation |
| title_short | Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation |
| title_sort | plasmatic membrane expression of adhesion molecules in human cardiac progenitor stem cells might explain their superior cell engraftment after cell transplantation |
| url | http://dx.doi.org/10.1155/2020/8872009 |
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