Automated pixel-based quantification of porcine circovirus 2 genome in formalin-fixed, paraffin-embedded tissues using in situ hybridisation

Automated pixel-based quantification of porcine circovirus 2 genome in formalin-fixed, paraffin-embedded tissues using in situ hybridisation

IntroductionDetection of porcine circovirus 2 (PCV2) in lymphoid tissues is essential for diagnostic and research purposes. In situ hybridisation (ISH) enables the localisation of viral genomes in tissue sections but is traditionally assessed visually, which may introduce subjectivity.MethodsThis st...

Full description

Saved in:
Bibliographic Details
Main Authors: Mònica Sagrera, Àlex Cobos, Laura Garza-Moreno, Mónica Pérez, Gema García-Buendía, Eva Huerta, Anna Maria Llorens, David Espigares, Marina Sibila, Joaquim Segalés
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Veterinary Science
Subjects:
porcine circovirus 2
in situ hybridisation
qPCR
RNAScope®
digital pathology
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2025.1609897/full
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://www.frontiersin.org/articles/10.3389/fvets.2025.1609897/full

Similar Items