Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer
Abstract Background LncRNAs are critical regulators of bladder cancer (BLCA), and ferroptosis is a newly discovered cell death responsible for mediating apoptosis and tumorigenesis. The present study aims to establish a prognostic signature of differentially expressed ferroptosis-related lncRNAs (DE...
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| Format: | Article |
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Springer
2024-11-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-024-01564-2 |
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| author | Jing Zhou Li Zhang Hao Wu Sheng-Lin Gao Xiao-Ping Chen Li-Feng Zhang Cui-Ping Zhao Bing-Bing Wei Yu Bai |
| author_facet | Jing Zhou Li Zhang Hao Wu Sheng-Lin Gao Xiao-Ping Chen Li-Feng Zhang Cui-Ping Zhao Bing-Bing Wei Yu Bai |
| author_sort | Jing Zhou |
| collection | DOAJ |
| description | Abstract Background LncRNAs are critical regulators of bladder cancer (BLCA), and ferroptosis is a newly discovered cell death responsible for mediating apoptosis and tumorigenesis. The present study aims to establish a prognostic signature of differentially expressed ferroptosis-related lncRNAs (DEFRlncRNAs) and explore the DEFRlncRNA associated with NUPR1 in BLCA. Methods DEFRlncRNAs in BLCA patients were screened using univariate and multivariate Cox and LASSO regression analyses. In vitro experiments were performed to detect the regulatory effects of DEFRlncRNAs on BLCA cells. A prognostic signature of DEFRlncRNAs in BLCA was created and validated. Moreover, we used RNA-binding protein immunoprecipitation (RIP) to evaluate the correlated DEFRlncRNA with NUPR1. Results A prognostic signature involving 18 DEFRlncRNAs in BLCA was created. Overexpression of AL355353.2 or knockdown of AL136084.3 promoted apoptosis in 5637 and T24 cells in vitro. Results from Starbase database estimated that AL136084.3 was positively associated with NUPR1 (R = 0.229, p < 0.001). RIP analysis revealed the reciprocal binding of NUPR1 and AL136084.3 in BLCA. Conclusion The identified FRlncRNA pair signature has a good prognostic and clinical predictive value. The ferroptosis-related lncRNA AL136084.3 is correlated with NUPR1 in BLCA. |
| format | Article |
| id | doaj-art-50e0b37d3db141bf9cadc4241a019b01 |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-50e0b37d3db141bf9cadc4241a019b012024-12-01T12:32:38ZengSpringerDiscover Oncology2730-60112024-11-0115111910.1007/s12672-024-01564-2Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancerJing Zhou0Li Zhang1Hao Wu2Sheng-Lin Gao3Xiao-Ping Chen4Li-Feng Zhang5Cui-Ping Zhao6Bing-Bing Wei7Yu Bai8Department of Medical Oncology, Affiliated Hospital of Jiangnan UniversityDepartment of Urology, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical UniversityDepartment of Urology, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical UniversityDepartment of Urology, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical UniversityDepartment of Medical Oncology, Affiliated Hospital of Jiangnan UniversityDepartment of Urology, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical UniversityDepartment of Geriatrics, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical UniversityDepartment of Urology, The Affiliated Wuxi People’s Hospital of Nanjing Medical UniversityDepartment of Urology, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical UniversityAbstract Background LncRNAs are critical regulators of bladder cancer (BLCA), and ferroptosis is a newly discovered cell death responsible for mediating apoptosis and tumorigenesis. The present study aims to establish a prognostic signature of differentially expressed ferroptosis-related lncRNAs (DEFRlncRNAs) and explore the DEFRlncRNA associated with NUPR1 in BLCA. Methods DEFRlncRNAs in BLCA patients were screened using univariate and multivariate Cox and LASSO regression analyses. In vitro experiments were performed to detect the regulatory effects of DEFRlncRNAs on BLCA cells. A prognostic signature of DEFRlncRNAs in BLCA was created and validated. Moreover, we used RNA-binding protein immunoprecipitation (RIP) to evaluate the correlated DEFRlncRNA with NUPR1. Results A prognostic signature involving 18 DEFRlncRNAs in BLCA was created. Overexpression of AL355353.2 or knockdown of AL136084.3 promoted apoptosis in 5637 and T24 cells in vitro. Results from Starbase database estimated that AL136084.3 was positively associated with NUPR1 (R = 0.229, p < 0.001). RIP analysis revealed the reciprocal binding of NUPR1 and AL136084.3 in BLCA. Conclusion The identified FRlncRNA pair signature has a good prognostic and clinical predictive value. The ferroptosis-related lncRNA AL136084.3 is correlated with NUPR1 in BLCA.https://doi.org/10.1007/s12672-024-01564-2FerroptosislncRNABladder cancerPrognosis |
| spellingShingle | Jing Zhou Li Zhang Hao Wu Sheng-Lin Gao Xiao-Ping Chen Li-Feng Zhang Cui-Ping Zhao Bing-Bing Wei Yu Bai Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer Discover Oncology Ferroptosis lncRNA Bladder cancer Prognosis |
| title | Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer |
| title_full | Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer |
| title_fullStr | Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer |
| title_full_unstemmed | Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer |
| title_short | Ferroptosis-related lncRNA AL136084.3 is associated with NUPR1 in bladder cancer |
| title_sort | ferroptosis related lncrna al136084 3 is associated with nupr1 in bladder cancer |
| topic | Ferroptosis lncRNA Bladder cancer Prognosis |
| url | https://doi.org/10.1007/s12672-024-01564-2 |
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