Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism
Abstract Background Monoacylglycerol lipase (MAGL) belongs to the serine hydrolase family; it catalyzes MAG to produce glycerol and free fatty acids (FFAs), which is the final step in triacylglycerol (TAG) hydrolysis. The effects of MAGL on comprehensive lipid metabolism and plant growth and develop...
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| Language: | English |
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BMC
2024-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-06017-0 |
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| author | Yihua Zhan Jing Wang Xuan Zhao Zhifu Zheng Yi Gan |
| author_facet | Yihua Zhan Jing Wang Xuan Zhao Zhifu Zheng Yi Gan |
| author_sort | Yihua Zhan |
| collection | DOAJ |
| description | Abstract Background Monoacylglycerol lipase (MAGL) belongs to the serine hydrolase family; it catalyzes MAG to produce glycerol and free fatty acids (FFAs), which is the final step in triacylglycerol (TAG) hydrolysis. The effects of MAGL on comprehensive lipid metabolism and plant growth and development have not been elucidated, especially in Arachis hypogaea, an important oil crop. Results Herein, AhMAGL3b encoding a protein with both hydrolase and acyltransferase regions, a member of MAGL gene family, was cloned and overexpressed in Arabidopsis thaliana. A total of 9 homozygous T3 generation transgenic lines were obtained. Compared with wild type (WT), overexpression (OE) of AhMAGL3b had no obvious growth inhibition by investigation of agronomic traits, including growth and photosynthetic parameters. The leaf fatty acid (FA) content was increased by 12.1–27.4% in AhMAGL3b-OE lines, while seed oil content was decreased by 10.7–17.3%. Furthermore, the overexpression of AhMAGL3b resulted in higher soluble sugar and starch content, and lower total soluble protein content in both leaves and seeds. Additionally, during seed germination, AhMAGL3b-OE seeds were more dormant than that of WT and the sensitivity to abscisic acid (ABA) treatment was decreased. Conclusions Taken together, our results indicate that AhMAGL3b is involved in homeostasis among carbohydrates, lipids and protein in A. hypogaea. |
| format | Article |
| id | doaj-art-5054f2f584fa4323946a460cf30f43da |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-5054f2f584fa4323946a460cf30f43da2025-01-05T12:13:10ZengBMCBMC Plant Biology1471-22292024-12-0124111110.1186/s12870-024-06017-0Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolismYihua Zhan0Jing Wang1Xuan Zhao2Zhifu Zheng3Yi Gan4College of Advanced Agricultural Sciences, Zhejiang A & F UniversityCollege of Advanced Agricultural Sciences, Zhejiang A & F UniversityCollege of Advanced Agricultural Sciences, Zhejiang A & F UniversityCollege of Advanced Agricultural Sciences, Zhejiang A & F UniversityCollege of Advanced Agricultural Sciences, Zhejiang A & F UniversityAbstract Background Monoacylglycerol lipase (MAGL) belongs to the serine hydrolase family; it catalyzes MAG to produce glycerol and free fatty acids (FFAs), which is the final step in triacylglycerol (TAG) hydrolysis. The effects of MAGL on comprehensive lipid metabolism and plant growth and development have not been elucidated, especially in Arachis hypogaea, an important oil crop. Results Herein, AhMAGL3b encoding a protein with both hydrolase and acyltransferase regions, a member of MAGL gene family, was cloned and overexpressed in Arabidopsis thaliana. A total of 9 homozygous T3 generation transgenic lines were obtained. Compared with wild type (WT), overexpression (OE) of AhMAGL3b had no obvious growth inhibition by investigation of agronomic traits, including growth and photosynthetic parameters. The leaf fatty acid (FA) content was increased by 12.1–27.4% in AhMAGL3b-OE lines, while seed oil content was decreased by 10.7–17.3%. Furthermore, the overexpression of AhMAGL3b resulted in higher soluble sugar and starch content, and lower total soluble protein content in both leaves and seeds. Additionally, during seed germination, AhMAGL3b-OE seeds were more dormant than that of WT and the sensitivity to abscisic acid (ABA) treatment was decreased. Conclusions Taken together, our results indicate that AhMAGL3b is involved in homeostasis among carbohydrates, lipids and protein in A. hypogaea.https://doi.org/10.1186/s12870-024-06017-0Arachis hypogaeaMAGLTAG metabolismGerminationABA |
| spellingShingle | Yihua Zhan Jing Wang Xuan Zhao Zhifu Zheng Yi Gan Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism BMC Plant Biology Arachis hypogaea MAGL TAG metabolism Germination ABA |
| title | Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism |
| title_full | Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism |
| title_fullStr | Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism |
| title_full_unstemmed | Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism |
| title_short | Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism |
| title_sort | arachis hypogaea monoacylglycerol lipase ahmagl3b participates in lipid metabolism |
| topic | Arachis hypogaea MAGL TAG metabolism Germination ABA |
| url | https://doi.org/10.1186/s12870-024-06017-0 |
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