Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i>
Drought is one of the most serious environmental stresses affecting crop production. NAC transcription factors play a crucial role in responding to various abiotic stresses in plants. Here, we identified a maize NAC transcription factor, <i>ZmSNAC06</i>, between drought-tolerant and drou...
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2024-12-01
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| Series: | Plants |
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| author | Fei Wang Yong Chen Ruisi Yang Ping Luo Houwen Wang Runze Zhang Wenzhe Li Ke Yang Xinlong Xu Zhuanfang Hao Xinhai Li |
| author_facet | Fei Wang Yong Chen Ruisi Yang Ping Luo Houwen Wang Runze Zhang Wenzhe Li Ke Yang Xinlong Xu Zhuanfang Hao Xinhai Li |
| author_sort | Fei Wang |
| collection | DOAJ |
| description | Drought is one of the most serious environmental stresses affecting crop production. NAC transcription factors play a crucial role in responding to various abiotic stresses in plants. Here, we identified a maize NAC transcription factor, <i>ZmSNAC06</i>, between drought-tolerant and drought-sensitive inbred lines through RNA-seq analysis and characterized its function in <i>Arabidopsis</i>. <i>ZmSNAC06</i> had five transcripts, of which <i>ZmSNAC06</i>-T02 had a typical NAC domain, while ZmSNAC06-P02 was localized in the nucleus of maize protoplasts and had transactivation activity in yeasts. The expression of <i>ZmSNAC06</i> in maize was induced by drought. The overexpression of <i>ZmSNAC06</i>-T02 in <i>Arabidopsis</i> resulted in hypersensitivity to abscisic acid (ABA) at the germination stage, and overexpression lines exhibited higher survival rates and higher antioxidant enzyme activities compared with the wild-type under drought stress. These results suggest that <i>ZmSNAC06</i> acts as a positive regulator in drought tolerance and may be used to improve drought tolerance in crops. |
| format | Article |
| id | doaj-art-4fb175adc1d6492fa464daf3c3995755 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-4fb175adc1d6492fa464daf3c39957552025-01-10T13:19:29ZengMDPI AGPlants2223-77472024-12-011411210.3390/plants14010012Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i>Fei Wang0Yong Chen1Ruisi Yang2Ping Luo3Houwen Wang4Runze Zhang5Wenzhe Li6Ke Yang7Xinlong Xu8Zhuanfang Hao9Xinhai Li10State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaDrought is one of the most serious environmental stresses affecting crop production. NAC transcription factors play a crucial role in responding to various abiotic stresses in plants. Here, we identified a maize NAC transcription factor, <i>ZmSNAC06</i>, between drought-tolerant and drought-sensitive inbred lines through RNA-seq analysis and characterized its function in <i>Arabidopsis</i>. <i>ZmSNAC06</i> had five transcripts, of which <i>ZmSNAC06</i>-T02 had a typical NAC domain, while ZmSNAC06-P02 was localized in the nucleus of maize protoplasts and had transactivation activity in yeasts. The expression of <i>ZmSNAC06</i> in maize was induced by drought. The overexpression of <i>ZmSNAC06</i>-T02 in <i>Arabidopsis</i> resulted in hypersensitivity to abscisic acid (ABA) at the germination stage, and overexpression lines exhibited higher survival rates and higher antioxidant enzyme activities compared with the wild-type under drought stress. These results suggest that <i>ZmSNAC06</i> acts as a positive regulator in drought tolerance and may be used to improve drought tolerance in crops.https://www.mdpi.com/2223-7747/14/1/12maize (<i>Zea mays</i> L.)<i>ZmSNAC06</i>transcription factordrought tolerance |
| spellingShingle | Fei Wang Yong Chen Ruisi Yang Ping Luo Houwen Wang Runze Zhang Wenzhe Li Ke Yang Xinlong Xu Zhuanfang Hao Xinhai Li Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i> Plants maize (<i>Zea mays</i> L.) <i>ZmSNAC06</i> transcription factor drought tolerance |
| title | Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i> |
| title_full | Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i> |
| title_fullStr | Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i> |
| title_full_unstemmed | Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i> |
| title_short | Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i> |
| title_sort | identification of i zmsnac06 i a maize nac family transcription factor with multiple transcripts conferring drought tolerance in i arabidopsis i |
| topic | maize (<i>Zea mays</i> L.) <i>ZmSNAC06</i> transcription factor drought tolerance |
| url | https://www.mdpi.com/2223-7747/14/1/12 |
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