Rapid molecular detection of Senecavirus A based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and CRISPR/Cas12a

Rapid molecular detection of Senecavirus A based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and CRISPR/Cas12a

IntroductionSenecavirus A (SVA), an emerging vesicular pathogen, is responsible for porcine idiopathic vesicular disease (PIVD). This disease is closely associated with porcine vesicular disease and acute neonatal piglet mortality, presenting a substantial threat to the global swine industry. At pre...

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Bibliographic Details
Main Authors: Chenghui Jiang, Huibao Wang, Rongxia Guo, Rui Yang, Xiaoming Li, Ping Liu, Jing Wang, Jincai Yang, Yanyan Chang, Qiaoying Zeng
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Senecavirus A
RT-LAMP
CRISPR/Cas12a
nucleic acid detection
visualisation
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2025.1451125/full
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