Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection
Molecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube....
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Main Authors: | Marcela Pereira Salazar, Juliana Figueiredo da Costa Lima Suassuna Monteiro, Wlisses Henrique Veloso Carvalho-Silva, George Tadeu Nunes Diniz, Roberto Pereira Werkhauser, Lílian Maria Lapa Montenegro, Haiana Charifker Schindler |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2024-06-01
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Series: | BioTechniques |
Subjects: | |
Online Access: | https://www.tandfonline.com/doi/10.2144/btn-2023-0080 |
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