Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze]
Bud blight caused by Pseudomonas syringae is a serious disease affecting tea plants and causing severe damage to production output and quality. Phages play an important role in controlling the development of bacterial diseases in plants. Previous studies have shown that the tolerance of phage-treate...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1525040/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556714160128000 |
|---|---|
| author | Li Liu Anqi Huang Hua Zhang Yubao Li Lei Wang Lei Wang |
| author_facet | Li Liu Anqi Huang Hua Zhang Yubao Li Lei Wang Lei Wang |
| author_sort | Li Liu |
| collection | DOAJ |
| description | Bud blight caused by Pseudomonas syringae is a serious disease affecting tea plants and causing severe damage to production output and quality. Phages play an important role in controlling the development of bacterial diseases in plants. Previous studies have shown that the tolerance of phage-treated tea plants to bud blight was notably greater compared with that of the control group. In the present study, we determined the effect of bacteriophage therapy on physiological and biochemical parameters of tea leaves. Transmission electron microscopy (TEM) was used to analyze the cellular structure of tea leaves, and bioinformatics was used to analyze the phage. Results revealed that bacteriophage treatment can enhance the expression of antioxidant enzyme genes (CsSOD, CsCAT, and CsPOD). The levels of osmotic adjustment compounds, including proline and soluble sugars, were also elevated, suggesting that bacteriophage enhances the osmotic adjustment capacity in tea plants. TEM analysis revealed that the integrity of the cell structure of the tea leaves treated with phage was notably better compared with that of the control group. Interestingly, we also observed that the phage lysed the animal pathogen Salmonella as well as the plant pathogen P. syringae. Using NCBI BLASTn to compare the entire genome with other nucleotide sequences, we found that the phage LDT325 exhibited cross-species characteristics that had not been previously reported. In summary, our findings demonstrate that bacteriophages can protect tea plants from damage caused by bacterial diseases by regulating antioxidant systems. |
| format | Article |
| id | doaj-art-4e6827a6a1c54b8f9437b2a1a22d90c8 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-4e6827a6a1c54b8f9437b2a1a22d90c82025-01-07T06:41:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.15250401525040Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze]Li Liu0Anqi Huang1Hua Zhang2Yubao Li3Lei Wang4Lei Wang5College of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng, ChinaSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng, ChinaSchool of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng, ChinaNational Key Laboratory of Macromolecular Drug Development and Manufacturing, Liaocheng University, Liaocheng, ChinaBud blight caused by Pseudomonas syringae is a serious disease affecting tea plants and causing severe damage to production output and quality. Phages play an important role in controlling the development of bacterial diseases in plants. Previous studies have shown that the tolerance of phage-treated tea plants to bud blight was notably greater compared with that of the control group. In the present study, we determined the effect of bacteriophage therapy on physiological and biochemical parameters of tea leaves. Transmission electron microscopy (TEM) was used to analyze the cellular structure of tea leaves, and bioinformatics was used to analyze the phage. Results revealed that bacteriophage treatment can enhance the expression of antioxidant enzyme genes (CsSOD, CsCAT, and CsPOD). The levels of osmotic adjustment compounds, including proline and soluble sugars, were also elevated, suggesting that bacteriophage enhances the osmotic adjustment capacity in tea plants. TEM analysis revealed that the integrity of the cell structure of the tea leaves treated with phage was notably better compared with that of the control group. Interestingly, we also observed that the phage lysed the animal pathogen Salmonella as well as the plant pathogen P. syringae. Using NCBI BLASTn to compare the entire genome with other nucleotide sequences, we found that the phage LDT325 exhibited cross-species characteristics that had not been previously reported. In summary, our findings demonstrate that bacteriophages can protect tea plants from damage caused by bacterial diseases by regulating antioxidant systems.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1525040/fullCamellia sinensisPseudomonas syringaebacteriophagephysiological charactersantioxidant enzyme |
| spellingShingle | Li Liu Anqi Huang Hua Zhang Yubao Li Lei Wang Lei Wang Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze] Frontiers in Microbiology Camellia sinensis Pseudomonas syringae bacteriophage physiological characters antioxidant enzyme |
| title | Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze] |
| title_full | Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze] |
| title_fullStr | Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze] |
| title_full_unstemmed | Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze] |
| title_short | Bacteriophage LDT325 enhances Pseudomonas syringae tolerance by improving antioxidant defense in tea plant [Camellia sinensis (L.) O. Kuntze] |
| title_sort | bacteriophage ldt325 enhances pseudomonas syringae tolerance by improving antioxidant defense in tea plant camellia sinensis l o kuntze |
| topic | Camellia sinensis Pseudomonas syringae bacteriophage physiological characters antioxidant enzyme |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1525040/full |
| work_keys_str_mv | AT liliu bacteriophageldt325enhancespseudomonassyringaetolerancebyimprovingantioxidantdefenseinteaplantcamelliasinensislokuntze AT anqihuang bacteriophageldt325enhancespseudomonassyringaetolerancebyimprovingantioxidantdefenseinteaplantcamelliasinensislokuntze AT huazhang bacteriophageldt325enhancespseudomonassyringaetolerancebyimprovingantioxidantdefenseinteaplantcamelliasinensislokuntze AT yubaoli bacteriophageldt325enhancespseudomonassyringaetolerancebyimprovingantioxidantdefenseinteaplantcamelliasinensislokuntze AT leiwang bacteriophageldt325enhancespseudomonassyringaetolerancebyimprovingantioxidantdefenseinteaplantcamelliasinensislokuntze AT leiwang bacteriophageldt325enhancespseudomonassyringaetolerancebyimprovingantioxidantdefenseinteaplantcamelliasinensislokuntze |