Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway
Abstract Concerted migration of basal stem cells (BCs) in the airway, also known as epithelial fluidization, has been implicated in epithelial repair after injury. How BC migration is regulated, and how it influences the success of epithelial repair, remains unclear. Here we have identified non-cano...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-59320-1 |
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| _version_ | 1849314617570885632 |
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| author | Daniel Jun-Kit Hu Xiaoyu Tracy Cai Jesse Simons Jina Yun Justin Elstrott Heinrich Jasper |
| author_facet | Daniel Jun-Kit Hu Xiaoyu Tracy Cai Jesse Simons Jina Yun Justin Elstrott Heinrich Jasper |
| author_sort | Daniel Jun-Kit Hu |
| collection | DOAJ |
| description | Abstract Concerted migration of basal stem cells (BCs) in the airway, also known as epithelial fluidization, has been implicated in epithelial repair after injury. How BC migration is regulated, and how it influences the success of epithelial repair, remains unclear. Here we have identified non-canonical Wnt signaling through Ptk7, Fzd7, and YAP as a critical regulator of BC migration in the mouse trachea. Using live imaging and genetic studies in the mouse, we find that Ptk7 is required for the concerted movement of BCs after injury, and that this requirement extends to BC proliferation and subsequent restoration of epithelial homeostasis after injury. We demonstrate that Ptk7 exerts this function in conjunction with Wnt5a and Fzd7, and through YAP activation in BCs. Our data provide mechanistic insight into the regulation of epithelial repair in the airway. |
| format | Article |
| id | doaj-art-4d8093d1e67d4c1ab4b8d921d1b85b6a |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-4d8093d1e67d4c1ab4b8d921d1b85b6a2025-08-20T03:52:24ZengNature PortfolioNature Communications2041-17232025-05-0116111310.1038/s41467-025-59320-1Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airwayDaniel Jun-Kit Hu0Xiaoyu Tracy Cai1Jesse Simons2Jina Yun3Justin Elstrott4Heinrich Jasper5Regenerative Medicine, Genentech Inc.Regenerative Medicine, Genentech Inc.Regenerative Medicine, Genentech Inc.Regenerative Medicine, Genentech Inc.Translational Imaging, Genentech Inc.Regenerative Medicine, Genentech Inc.Abstract Concerted migration of basal stem cells (BCs) in the airway, also known as epithelial fluidization, has been implicated in epithelial repair after injury. How BC migration is regulated, and how it influences the success of epithelial repair, remains unclear. Here we have identified non-canonical Wnt signaling through Ptk7, Fzd7, and YAP as a critical regulator of BC migration in the mouse trachea. Using live imaging and genetic studies in the mouse, we find that Ptk7 is required for the concerted movement of BCs after injury, and that this requirement extends to BC proliferation and subsequent restoration of epithelial homeostasis after injury. We demonstrate that Ptk7 exerts this function in conjunction with Wnt5a and Fzd7, and through YAP activation in BCs. Our data provide mechanistic insight into the regulation of epithelial repair in the airway.https://doi.org/10.1038/s41467-025-59320-1 |
| spellingShingle | Daniel Jun-Kit Hu Xiaoyu Tracy Cai Jesse Simons Jina Yun Justin Elstrott Heinrich Jasper Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway Nature Communications |
| title | Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway |
| title_full | Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway |
| title_fullStr | Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway |
| title_full_unstemmed | Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway |
| title_short | Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway |
| title_sort | non canonical wnt signaling promotes epithelial fluidization in the repairing airway |
| url | https://doi.org/10.1038/s41467-025-59320-1 |
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