Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids
Abstract Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoid characterization predominantly rely on molecular a...
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Nature Portfolio
2024-09-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-72038-2 |
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| author | Caroline E. Serafini Seleipiri Charles Paloma Casteleiro Costa Weibo Niu Brian Cheng Zhexing Wen Hang Lu Francisco E. Robles |
| author_facet | Caroline E. Serafini Seleipiri Charles Paloma Casteleiro Costa Weibo Niu Brian Cheng Zhexing Wen Hang Lu Francisco E. Robles |
| author_sort | Caroline E. Serafini |
| collection | DOAJ |
| description | Abstract Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoid characterization predominantly rely on molecular analysis methods, which are expensive, time-consuming, labor-intensive, and involve the destruction of the valuable three-dimensional (3D) architecture of the organoids. This reliance on end-point assays makes it challenging to assess cellular and subcellular events occurring during organoid development in their 3D context. As a result, the long developmental processes are not monitored nor assessed. The ability to perform non-invasive assays is critical for longitudinally assessing features of organoid development during culture. In this paper, we demonstrate a label-free high-content imaging approach for observing changes in organoid morphology and structural changes occurring at the cellular and subcellular level. Enabled by microfluidic-based culture of 3D cell systems and a novel 3D quantitative phase imaging method, we demonstrate the ability to perform non-destructive high-resolution quantitative image analysis of the organoid. The highlighted results demonstrated in this paper provide a new approach to performing live, non-destructive monitoring of organoid systems during culture. |
| format | Article |
| id | doaj-art-4d0b0f8e141c41bea1ae88a5397d8de1 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4d0b0f8e141c41bea1ae88a5397d8de12024-12-15T12:08:51ZengNature PortfolioScientific Reports2045-23222024-09-0114111510.1038/s41598-024-72038-2Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoidsCaroline E. Serafini0Seleipiri Charles1Paloma Casteleiro Costa2Weibo Niu3Brian Cheng4Zhexing Wen5Hang Lu6Francisco E. Robles7George W. Woodruff School of Mechanical Engineering, Georgia Institute of TechnologyGeorgia Institute of Technology, Interdisciplinary Program in BioengineeringGeorgia Institute of Technology, School of Electrical and Computer EngineeringDepartment of Psychiatry and Behavioral Sciences, Emory University School of MedicineWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory UniversityDepartment of Psychiatry and Behavioral Sciences, Emory University School of MedicineGeorgia Institute of Technology, Interdisciplinary Program in BioengineeringGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of TechnologyAbstract Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoid characterization predominantly rely on molecular analysis methods, which are expensive, time-consuming, labor-intensive, and involve the destruction of the valuable three-dimensional (3D) architecture of the organoids. This reliance on end-point assays makes it challenging to assess cellular and subcellular events occurring during organoid development in their 3D context. As a result, the long developmental processes are not monitored nor assessed. The ability to perform non-invasive assays is critical for longitudinally assessing features of organoid development during culture. In this paper, we demonstrate a label-free high-content imaging approach for observing changes in organoid morphology and structural changes occurring at the cellular and subcellular level. Enabled by microfluidic-based culture of 3D cell systems and a novel 3D quantitative phase imaging method, we demonstrate the ability to perform non-destructive high-resolution quantitative image analysis of the organoid. The highlighted results demonstrated in this paper provide a new approach to performing live, non-destructive monitoring of organoid systems during culture.https://doi.org/10.1038/s41598-024-72038-2MesofluidicsBrain organoidsQuantitative phase imaging systemsLive imagingNeurodevelopmental disordersNon-invasive imaging |
| spellingShingle | Caroline E. Serafini Seleipiri Charles Paloma Casteleiro Costa Weibo Niu Brian Cheng Zhexing Wen Hang Lu Francisco E. Robles Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids Scientific Reports Mesofluidics Brain organoids Quantitative phase imaging systems Live imaging Neurodevelopmental disorders Non-invasive imaging |
| title | Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids |
| title_full | Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids |
| title_fullStr | Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids |
| title_full_unstemmed | Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids |
| title_short | Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids |
| title_sort | non invasive label free imaging analysis pipeline for in situ characterization of 3d brain organoids |
| topic | Mesofluidics Brain organoids Quantitative phase imaging systems Live imaging Neurodevelopmental disorders Non-invasive imaging |
| url | https://doi.org/10.1038/s41598-024-72038-2 |
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