A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans
Abstract Background To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of Streptococcus mutans, a key cariogenic pathogen in dental caries. Methods SB-10 was compared to Mitis Salivarius Bacitracin a...
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BMC
2025-08-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-06675-y |
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| author | Yuwen Fang Qizhao Ma Wei Zhao Nian Liu Lei Cheng Jing Chen Jing Zou |
| author_facet | Yuwen Fang Qizhao Ma Wei Zhao Nian Liu Lei Cheng Jing Chen Jing Zou |
| author_sort | Yuwen Fang |
| collection | DOAJ |
| description | Abstract Background To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of Streptococcus mutans, a key cariogenic pathogen in dental caries. Methods SB-10 was compared to Mitis Salivarius Bacitracin agar with 20% sucrose (MSB-20) for its ability to support S. mutans growth, promote distinctive colony morphology, and differentiate it from other oral bacteria. Sugar particle formation was assessed using wild-type and mutant strains of S. mutans lacking glucosyltransferases B and C (GtfB and GtfC). Liquid chromatography was performed to characterize the sugar particles around colonies. The specificity and clinical utility of SB-10 were further assessed by culturing clinical saliva samples and confirming S. mutans colonies via 16S rRNA sequencing. Results SB-10 supported robust S. mutans growth with larger colonies and distinctive sugar particle formation compared to MSB-20. GtfB and GtfC were essential for sugar particle deposition around colonies. SB-10 demonstrated high specificity by suppressing the growth of non-target oral bacteria and accurately isolating S. mutans from clinical saliva samples, with all colonies confirmed as S. mutans via 16S rRNA sequencing. Conclusions SB-10 is a novel selective medium that enhances the isolation and identification of S. mutans, characterized by unique sugar particles deposition and colony morphology. |
| format | Article |
| id | doaj-art-4b04a57b125b4587b98bca61484fbfd5 |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-4b04a57b125b4587b98bca61484fbfd52025-08-20T03:43:45ZengBMCBMC Oral Health1472-68312025-08-0125111010.1186/s12903-025-06675-yA novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutansYuwen Fang0Qizhao Ma1Wei Zhao2Nian Liu3Lei Cheng4Jing Chen5Jing Zou6State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityAbstract Background To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of Streptococcus mutans, a key cariogenic pathogen in dental caries. Methods SB-10 was compared to Mitis Salivarius Bacitracin agar with 20% sucrose (MSB-20) for its ability to support S. mutans growth, promote distinctive colony morphology, and differentiate it from other oral bacteria. Sugar particle formation was assessed using wild-type and mutant strains of S. mutans lacking glucosyltransferases B and C (GtfB and GtfC). Liquid chromatography was performed to characterize the sugar particles around colonies. The specificity and clinical utility of SB-10 were further assessed by culturing clinical saliva samples and confirming S. mutans colonies via 16S rRNA sequencing. Results SB-10 supported robust S. mutans growth with larger colonies and distinctive sugar particle formation compared to MSB-20. GtfB and GtfC were essential for sugar particle deposition around colonies. SB-10 demonstrated high specificity by suppressing the growth of non-target oral bacteria and accurately isolating S. mutans from clinical saliva samples, with all colonies confirmed as S. mutans via 16S rRNA sequencing. Conclusions SB-10 is a novel selective medium that enhances the isolation and identification of S. mutans, characterized by unique sugar particles deposition and colony morphology.https://doi.org/10.1186/s12903-025-06675-yDental cariesStreptococcus mutansSelective mediumSucrose-Bacitracin agarGlucosyltransferases |
| spellingShingle | Yuwen Fang Qizhao Ma Wei Zhao Nian Liu Lei Cheng Jing Chen Jing Zou A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans BMC Oral Health Dental caries Streptococcus mutans Selective medium Sucrose-Bacitracin agar Glucosyltransferases |
| title | A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans |
| title_full | A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans |
| title_fullStr | A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans |
| title_full_unstemmed | A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans |
| title_short | A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans |
| title_sort | novel selective medium sucrose bacitracin agar 10 for accurate isolation and identification of streptococcus mutans |
| topic | Dental caries Streptococcus mutans Selective medium Sucrose-Bacitracin agar Glucosyltransferases |
| url | https://doi.org/10.1186/s12903-025-06675-y |
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