A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans
Abstract Background To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of Streptococcus mutans, a key cariogenic pathogen in dental caries. Methods SB-10 was compared to Mitis Salivarius Bacitracin a...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-08-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-025-06675-y |
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| Summary: | Abstract Background To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of Streptococcus mutans, a key cariogenic pathogen in dental caries. Methods SB-10 was compared to Mitis Salivarius Bacitracin agar with 20% sucrose (MSB-20) for its ability to support S. mutans growth, promote distinctive colony morphology, and differentiate it from other oral bacteria. Sugar particle formation was assessed using wild-type and mutant strains of S. mutans lacking glucosyltransferases B and C (GtfB and GtfC). Liquid chromatography was performed to characterize the sugar particles around colonies. The specificity and clinical utility of SB-10 were further assessed by culturing clinical saliva samples and confirming S. mutans colonies via 16S rRNA sequencing. Results SB-10 supported robust S. mutans growth with larger colonies and distinctive sugar particle formation compared to MSB-20. GtfB and GtfC were essential for sugar particle deposition around colonies. SB-10 demonstrated high specificity by suppressing the growth of non-target oral bacteria and accurately isolating S. mutans from clinical saliva samples, with all colonies confirmed as S. mutans via 16S rRNA sequencing. Conclusions SB-10 is a novel selective medium that enhances the isolation and identification of S. mutans, characterized by unique sugar particles deposition and colony morphology. |
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| ISSN: | 1472-6831 |