Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties
Abstract Background The mucus from fish and gastropods contains a wide range of bioactive molecules with biomedical properties. The fish and gastropods were collected from Oragadam lake, Kanchipuram district, Tamil Nadu, India. In this study, we wanted to examine the anticancer potential of fish and...
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SpringerOpen
2025-01-01
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| Series: | Journal of Basic and Applied Zoology |
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| Online Access: | https://doi.org/10.1186/s41936-024-00423-4 |
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| author | Elumalai Devan Hemavathi Maduraiveeran Kavitha Raja Arulvasu Chinnasamy Govindan Sivalingam Sathya Balaji Vinoth Neelakandan Sharon Agnita Murugan Rajaram |
| author_facet | Elumalai Devan Hemavathi Maduraiveeran Kavitha Raja Arulvasu Chinnasamy Govindan Sivalingam Sathya Balaji Vinoth Neelakandan Sharon Agnita Murugan Rajaram |
| author_sort | Elumalai Devan |
| collection | DOAJ |
| description | Abstract Background The mucus from fish and gastropods contains a wide range of bioactive molecules with biomedical properties. The fish and gastropods were collected from Oragadam lake, Kanchipuram district, Tamil Nadu, India. In this study, we wanted to examine the anticancer potential of fish and gastropods mucus. The biochemical components of the crude mucus were screened. Results The biochemical analysis showed that the mucus of Anabas testudineus and Pila virens contained a high level of carbohydrates (2.8 and 1.5 mg/ml), the mucus of Channa striata contained a high level of lipids (0.9 mg/ml), and the mucus of Helix aspersa contained a high level of protein (1.3 mg/ml). The results showed morphological variations in the HT-29 cells upon treatment with crude mucus. Upon 24 h of gestation, the frozen cells began to shrink and seem round in shape. Using the MTT assay, the mucus crude extract was evaluated for its anticancer properties against the human colon cancer cell line (HT 29). The inhibitory concentration (IC50) was determined at 100 µg/ml after 24 h. Using specific staining techniques; fluorescent microscopy was utilized to examine the cell morphology and early and late apoptotic stages. Propidium iodide staining showed nuclear damage followed by DNA damage. This showed that the rudimentary mucus could prompt cell death and increased the number of fragments and mucus concentration, respectively. Conclusion This study showed that the edible or commercially important fish and gastropod mucus have potential anticancer activity against HT-29 cancer cells. |
| format | Article |
| id | doaj-art-44f011dc0806492e8a6827337cae8b7c |
| institution | Kabale University |
| issn | 2090-990X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj-art-44f011dc0806492e8a6827337cae8b7c2025-01-12T12:34:05ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2025-01-018611910.1186/s41936-024-00423-4Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical propertiesElumalai Devan0Hemavathi Maduraiveeran1Kavitha Raja2Arulvasu Chinnasamy3Govindan Sivalingam4Sathya Balaji5Vinoth Neelakandan6Sharon Agnita7Murugan Rajaram8PG Department of Zoology, Pachaiyappa’s College for MenDepartment of Zoology, Pachaiyappa’s College for WomenDepartment of Zoology, University of MadrasDepartment of Zoology, University of MadrasPG Department of Zoology, Pachaiyappa’s College for MenPG Department of Zoology, Pachaiyappa’s College for MenPG Department of Zoology, Pachaiyappa’s College for MenDNA Barcoding Lab, Zoological Survey of India, Southern Regional CentreCoastal System Research Division, MS. Swaminathan Research Foundation (MSSRF)Abstract Background The mucus from fish and gastropods contains a wide range of bioactive molecules with biomedical properties. The fish and gastropods were collected from Oragadam lake, Kanchipuram district, Tamil Nadu, India. In this study, we wanted to examine the anticancer potential of fish and gastropods mucus. The biochemical components of the crude mucus were screened. Results The biochemical analysis showed that the mucus of Anabas testudineus and Pila virens contained a high level of carbohydrates (2.8 and 1.5 mg/ml), the mucus of Channa striata contained a high level of lipids (0.9 mg/ml), and the mucus of Helix aspersa contained a high level of protein (1.3 mg/ml). The results showed morphological variations in the HT-29 cells upon treatment with crude mucus. Upon 24 h of gestation, the frozen cells began to shrink and seem round in shape. Using the MTT assay, the mucus crude extract was evaluated for its anticancer properties against the human colon cancer cell line (HT 29). The inhibitory concentration (IC50) was determined at 100 µg/ml after 24 h. Using specific staining techniques; fluorescent microscopy was utilized to examine the cell morphology and early and late apoptotic stages. Propidium iodide staining showed nuclear damage followed by DNA damage. This showed that the rudimentary mucus could prompt cell death and increased the number of fragments and mucus concentration, respectively. Conclusion This study showed that the edible or commercially important fish and gastropod mucus have potential anticancer activity against HT-29 cancer cells.https://doi.org/10.1186/s41936-024-00423-4FishGastropodsMucusHT-29 anticancer |
| spellingShingle | Elumalai Devan Hemavathi Maduraiveeran Kavitha Raja Arulvasu Chinnasamy Govindan Sivalingam Sathya Balaji Vinoth Neelakandan Sharon Agnita Murugan Rajaram Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties Journal of Basic and Applied Zoology Fish Gastropods Mucus HT-29 anticancer |
| title | Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties |
| title_full | Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties |
| title_fullStr | Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties |
| title_full_unstemmed | Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties |
| title_short | Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties |
| title_sort | antitumor activity of edible fishes channa striata and anabas testudineus and gastropods helix aspersa and pila virens rudimentary mucus against ht 29 cell line and its biochemical properties |
| topic | Fish Gastropods Mucus HT-29 anticancer |
| url | https://doi.org/10.1186/s41936-024-00423-4 |
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