Study of Salidroside on Expression of Synaptophysin and Tau in Rats after Local Cerebral Ischemia-reperfusion Injury
Objective:To investigate the effect of salidroside on the expression of synaptophysin (SYN) and Tau in rats after cerebral ischemia-reperfusion injury.Methods:A total of 36 healthy adult male Sprague-Dawley rats were randomly divided into sham group (<italic>n</italic>=12) and model grou...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Office of Rehabilitation Medicine
2017-12-01
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| Series: | 康复学报 |
| Subjects: | |
| Online Access: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2017.06023 |
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| Summary: | Objective:To investigate the effect of salidroside on the expression of synaptophysin (SYN) and Tau in rats after cerebral ischemia-reperfusion injury.Methods:A total of 36 healthy adult male Sprague-Dawley rats were randomly divided into sham group (<italic>n</italic>=12) and model group (<italic>n</italic>=24). Cerebral ischemia model of middle cerebral artery occlusion (MCAO) was established in rats of model group using suture method, and the nerves function score was determined by Zeal Longa score after the animal model was fully awakened. Then the successful model rats were randomly divided into model group and salidroside group, twelve rats in each group. With occluding of middle cerebral arteries for two hours and reperfusion for 24 hours, the sham group and model group were treated with normal saline at a dose of 50 mg/kg, and the salidroside group received salidroside at a dose of 50 mg/kg by intraperitoneal injection. All rats were sacrificed 24 hours after MCAO operation. The rats were treated with drugs one more time at two hours before sacrifice. The mRNA expression of SYN and Tau in ischemic brain tissue were detected by RT-qPCR, the protein expression of phosphorylated Tau at threonine 231 (p-Tau231) was detected by immunohistochemical method, and the protein expression of SYN and p-Tau231 in ischemic brain tissue were detected by Western Blot.Results:Compared with the sham group, SYN expression in model group was decreased (<italic>P</italic><0.05);SYN expression in salidroside group was increased compared with the model group (<italic>P</italic><0.05); p-Tau231 expression in model group was increased compared with the sham group (<italic>P</italic><0.01). p-Tau231 expression in salidroside group was decreased compared with model group (<italic>P</italic><0.01).Conclusion:Salidroside can increase the injury-induced downregulation of SYN when the injury caused by MCAO, and decrease hyperphosphorylation of Tau protein in the ipsilateral thalamus of rats after cerebral ischemia-reperfusion injury. |
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| ISSN: | 2096-0328 |