The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar rats
Background Skin aging is a complex biological phenomenon influenced by intrinsic and extrinsic factors. Photoaging can be prevented by applying phytochemicals that have sun-protective properties. This study aimed to evaluate the effect of saffron serum to restore collagen density and autophagy proc...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Trisakti University
2024-12-01
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| Series: | Universa Medicina |
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| Online Access: | https://univmed.org/ejurnal/index.php/medicina/article/view/1646 |
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| author | Chitra Octavia Julia Windi Gunadi Oeij Anindita Adhika Lani Ishak Diana Krisanti Jasaputra Alexandrina Everdine Rosali Ardo Sanjaya |
| author_facet | Chitra Octavia Julia Windi Gunadi Oeij Anindita Adhika Lani Ishak Diana Krisanti Jasaputra Alexandrina Everdine Rosali Ardo Sanjaya |
| author_sort | Chitra Octavia |
| collection | DOAJ |
| description |
Background
Skin aging is a complex biological phenomenon influenced by intrinsic and extrinsic factors. Photoaging can be prevented by applying phytochemicals that have sun-protective properties. This study aimed to evaluate the effect of saffron serum to restore collagen density and autophagy processes and reduce inflammatory gene expression in UVB-exposed Wistar rats.
Methods
An experimental laboratory study was conducted involving 20 male Wistar rats that were divided into 4 groups: control, UVB, UVB + base serum, UVB + saffron serum exposed to UVB radiation for 5 weeks with a total dose of 3100 mJ/cm2. The skin was extracted then underwent Masson Trichrome staining and real-time PCR to obtain collagen density and gene expression.
Results
The gene expression of MMP1, IL6, TNFá, LC3, and p62 was significantly increased in the UVB group compared to the control group. Topical administration of saffron serum significantly increased collagen density (p=0.001). Induction by UVB significantly increased LC3 (p=0.020) and p62 (p=0.030) gene expression, indicating an inhibition of autophagy. The saffron serum might modulate autophagy by increasing LC3, but not significant (p=0.495) and significantly decreasing p62 gene expression (p=0.001). As for MMP1, IL6, and TNFá, no significant decrease in gene expression was found in the UVB + saffron serum group compared to the UVB group.
Conclusion
Saffron serum increases collagen density and modulates autophagy in the skin of UVB-exposed Wistar rats. Inflammatory markers were increased after UVB induction, but no differences were found after saffron serum topical administration.
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| format | Article |
| id | doaj-art-41de24b6cfe247ec914856ca1c5664c4 |
| institution | Kabale University |
| issn | 2407-2230 1907-3062 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Faculty of Medicine Trisakti University |
| record_format | Article |
| series | Universa Medicina |
| spelling | doaj-art-41de24b6cfe247ec914856ca1c5664c42024-12-13T07:38:25ZengFaculty of Medicine Trisakti UniversityUniversa Medicina2407-22301907-30622024-12-0143310.18051/UnivMed.2024.v43.329-339The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar ratsChitra Octavia0Julia Windi Gunadi1Oeij Anindita Adhika2Lani Ishak3Diana Krisanti Jasaputra4Alexandrina Everdine Rosali5Ardo Sanjaya6Master Program of Skin Ageing and Aesthetic Medicine, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, IndonesiaMaster Program of Skin Ageing and Aesthetic Medicine, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia; Department of Physiology, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, IndonesiaDepartment of Anatomy, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, IndonesiaMaster Program of Skin Ageing and Aesthetic Medicine, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, IndonesiaMaster Program of Skin Ageing and Aesthetic Medicine, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia; Department of Pharmacology, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, IndonesiaMaster Program of Skin Ageing and Aesthetic Medicine, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, IndonesiaDepartment of Anatomy, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia Background Skin aging is a complex biological phenomenon influenced by intrinsic and extrinsic factors. Photoaging can be prevented by applying phytochemicals that have sun-protective properties. This study aimed to evaluate the effect of saffron serum to restore collagen density and autophagy processes and reduce inflammatory gene expression in UVB-exposed Wistar rats. Methods An experimental laboratory study was conducted involving 20 male Wistar rats that were divided into 4 groups: control, UVB, UVB + base serum, UVB + saffron serum exposed to UVB radiation for 5 weeks with a total dose of 3100 mJ/cm2. The skin was extracted then underwent Masson Trichrome staining and real-time PCR to obtain collagen density and gene expression. Results The gene expression of MMP1, IL6, TNFá, LC3, and p62 was significantly increased in the UVB group compared to the control group. Topical administration of saffron serum significantly increased collagen density (p=0.001). Induction by UVB significantly increased LC3 (p=0.020) and p62 (p=0.030) gene expression, indicating an inhibition of autophagy. The saffron serum might modulate autophagy by increasing LC3, but not significant (p=0.495) and significantly decreasing p62 gene expression (p=0.001). As for MMP1, IL6, and TNFá, no significant decrease in gene expression was found in the UVB + saffron serum group compared to the UVB group. Conclusion Saffron serum increases collagen density and modulates autophagy in the skin of UVB-exposed Wistar rats. Inflammatory markers were increased after UVB induction, but no differences were found after saffron serum topical administration. https://univmed.org/ejurnal/index.php/medicina/article/view/1646UVBcollagen densityMMP1IL6TNFαautophagy |
| spellingShingle | Chitra Octavia Julia Windi Gunadi Oeij Anindita Adhika Lani Ishak Diana Krisanti Jasaputra Alexandrina Everdine Rosali Ardo Sanjaya The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar rats Universa Medicina UVB collagen density MMP1 IL6 TNFα autophagy |
| title | The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar rats |
| title_full | The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar rats |
| title_fullStr | The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar rats |
| title_full_unstemmed | The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar rats |
| title_short | The effect of saffron serum on collagen density, inflammatory gene expression, and autophagy in UVB-exposed Wistar rats |
| title_sort | effect of saffron serum on collagen density inflammatory gene expression and autophagy in uvb exposed wistar rats |
| topic | UVB collagen density MMP1 IL6 TNFα autophagy |
| url | https://univmed.org/ejurnal/index.php/medicina/article/view/1646 |
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