Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic Aneurysms
Macrophage infiltration is a prominent feature of abdominal aortic aneurysm (AAA) progression. We used a combined imaging approach with bioluminescence (BLI) and magnetic resonance imaging (MRI) to study macrophage homing and accumulation in experimental AAA disease. Murine AAAs were created via int...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2012-03-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2011.00033 |
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| author | Noriyuki Miyama Monica M. Dua Geoffrey M. Schultz Hisanori Kosuge Masahiro Terashima Laura J. Pisani Ronald L. Dalman Michael V. McConnell |
| author_facet | Noriyuki Miyama Monica M. Dua Geoffrey M. Schultz Hisanori Kosuge Masahiro Terashima Laura J. Pisani Ronald L. Dalman Michael V. McConnell |
| author_sort | Noriyuki Miyama |
| collection | DOAJ |
| description | Macrophage infiltration is a prominent feature of abdominal aortic aneurysm (AAA) progression. We used a combined imaging approach with bioluminescence (BLI) and magnetic resonance imaging (MRI) to study macrophage homing and accumulation in experimental AAA disease. Murine AAAs were created via intra-aortic infusion of porcine pancreatic elastase. Mice were imaged over 14 days after injection of prepared peritoneal macrophages. For BLI, macrophages were from transgenic mice expressing luciferase. For MRI, macrophages were labeled with iron oxide particles. Macrophage accumulation during aneurysm progression was observed by in situ BLI and by in vivo 7T MRI. Mice were sacrificed after imaging for histologic analysis. In situ BLI ( n = 32) demonstrated high signal in the AAA by days 7 and 14, which correlated significantly with macrophage number and aortic diameter. In vivo 7T MRI ( n = 13) at day 14 demonstrated T 2 * signal loss in the AAA and not in sham mice. Immunohistochemistry and Prussian blue staining confirmed the presence of injected macrophages in the AAA. BLI and MRI provide complementary approaches to track macrophage homing and accumulation in experimental AAAs. Similar dual imaging strategies may aid the study of AAA biology and the evaluation of novel therapies. |
| format | Article |
| id | doaj-art-41522f6de8d245479af466055ca02802 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2012-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-41522f6de8d245479af466055ca028022025-01-03T00:10:43ZengSAGE PublishingMolecular Imaging1536-01212012-03-011110.2310/7290.2011.0003310.2310_7290.2011.00033Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic AneurysmsNoriyuki MiyamaMonica M. DuaGeoffrey M. SchultzHisanori KosugeMasahiro TerashimaLaura J. PisaniRonald L. DalmanMichael V. McConnellMacrophage infiltration is a prominent feature of abdominal aortic aneurysm (AAA) progression. We used a combined imaging approach with bioluminescence (BLI) and magnetic resonance imaging (MRI) to study macrophage homing and accumulation in experimental AAA disease. Murine AAAs were created via intra-aortic infusion of porcine pancreatic elastase. Mice were imaged over 14 days after injection of prepared peritoneal macrophages. For BLI, macrophages were from transgenic mice expressing luciferase. For MRI, macrophages were labeled with iron oxide particles. Macrophage accumulation during aneurysm progression was observed by in situ BLI and by in vivo 7T MRI. Mice were sacrificed after imaging for histologic analysis. In situ BLI ( n = 32) demonstrated high signal in the AAA by days 7 and 14, which correlated significantly with macrophage number and aortic diameter. In vivo 7T MRI ( n = 13) at day 14 demonstrated T 2 * signal loss in the AAA and not in sham mice. Immunohistochemistry and Prussian blue staining confirmed the presence of injected macrophages in the AAA. BLI and MRI provide complementary approaches to track macrophage homing and accumulation in experimental AAAs. Similar dual imaging strategies may aid the study of AAA biology and the evaluation of novel therapies.https://doi.org/10.2310/7290.2011.00033 |
| spellingShingle | Noriyuki Miyama Monica M. Dua Geoffrey M. Schultz Hisanori Kosuge Masahiro Terashima Laura J. Pisani Ronald L. Dalman Michael V. McConnell Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic Aneurysms Molecular Imaging |
| title | Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic Aneurysms |
| title_full | Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic Aneurysms |
| title_fullStr | Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic Aneurysms |
| title_full_unstemmed | Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic Aneurysms |
| title_short | Bioluminescence and Magnetic Resonance Imaging of Macrophage Homing to Experimental Abdominal Aortic Aneurysms |
| title_sort | bioluminescence and magnetic resonance imaging of macrophage homing to experimental abdominal aortic aneurysms |
| url | https://doi.org/10.2310/7290.2011.00033 |
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