MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy
This study aimed to elucidate the expression patterns of miR-33 and ARC in both a rat model of temporal lobe epilepsy (TLE) and human TLE patients, to explore the role of miR-33 in epilepsy onset through its regulation of ARC expression in the hippocampus. Our findings, supported by a Dual-Luciferas...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Neuroscience Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168010224001093 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841527156817002496 |
|---|---|
| author | Yuchen Xu Lily Zhang Yan Yan Wenbiao Xiao Wei Zou Zhaohui Luo Bo Xiao Hongyu Long |
| author_facet | Yuchen Xu Lily Zhang Yan Yan Wenbiao Xiao Wei Zou Zhaohui Luo Bo Xiao Hongyu Long |
| author_sort | Yuchen Xu |
| collection | DOAJ |
| description | This study aimed to elucidate the expression patterns of miR-33 and ARC in both a rat model of temporal lobe epilepsy (TLE) and human TLE patients, to explore the role of miR-33 in epilepsy onset through its regulation of ARC expression in the hippocampus. Our findings, supported by a Dual-Luciferase reporter assay, suggest that miR-33 can bind to the 3′ UTR region of ARC. We observed that miR-33 levels were reduced at 1 hour and 60 days post-seizure, while ARC expression notably increased at these time points. In the hippocampal CA1 and CA3 regions of post-seizure rats, ARC expression significantly exceeded that of control groups. Following the transfection of HEK cells with a miR-33 mimic, there was a decrease in both ARC mRNA and protein levels, whereas the group treated with a miR-33 inhibitor displayed the opposite effect. RNA sequencing in TLE patients revealed a similar miR-33 and ARC interaction. The regulation of Arc expression by miR-33 suggests that Arc may be a target gene of miR-33 in the context of epilepsy. Our findings indicate that miR-33 downregulation could contribute to the dysregulation of Arc expression observed in TLE, potentially influencing the disease process. Further studies are required to establish the exact role of miR-33-mediated Arc regulation in the development of epilepsy. |
| format | Article |
| id | doaj-art-40395f35aa164c2fa453847210bbdab6 |
| institution | Kabale University |
| issn | 0168-0102 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neuroscience Research |
| spelling | doaj-art-40395f35aa164c2fa453847210bbdab62025-01-16T04:28:16ZengElsevierNeuroscience Research0168-01022025-01-012101927MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsyYuchen Xu0Lily Zhang1Yan Yan2Wenbiao Xiao3Wei Zou4Zhaohui Luo5Bo Xiao6Hongyu Long7Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China; Corresponding authors.Neurology Department, Xiangya Hospital, Central South University, Changsha, Hunan, ChinaNeurology Department, Loudi Central Hospital, Loudi, Hunan, ChinaNeurology Department, Xiangya Hospital, Central South University, Changsha, Hunan, ChinaNHC Key Laboratory of Birth Defects Research, Prevention, and Treatment, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, ChinaNeurology Department, Xiangya Hospital, Central South University, Changsha, Hunan, ChinaNeurology Department, Xiangya Hospital, Central South University, Changsha, Hunan, ChinaNeurology Department, Xiangya Hospital, Central South University, Changsha, Hunan, China; Corresponding authors.This study aimed to elucidate the expression patterns of miR-33 and ARC in both a rat model of temporal lobe epilepsy (TLE) and human TLE patients, to explore the role of miR-33 in epilepsy onset through its regulation of ARC expression in the hippocampus. Our findings, supported by a Dual-Luciferase reporter assay, suggest that miR-33 can bind to the 3′ UTR region of ARC. We observed that miR-33 levels were reduced at 1 hour and 60 days post-seizure, while ARC expression notably increased at these time points. In the hippocampal CA1 and CA3 regions of post-seizure rats, ARC expression significantly exceeded that of control groups. Following the transfection of HEK cells with a miR-33 mimic, there was a decrease in both ARC mRNA and protein levels, whereas the group treated with a miR-33 inhibitor displayed the opposite effect. RNA sequencing in TLE patients revealed a similar miR-33 and ARC interaction. The regulation of Arc expression by miR-33 suggests that Arc may be a target gene of miR-33 in the context of epilepsy. Our findings indicate that miR-33 downregulation could contribute to the dysregulation of Arc expression observed in TLE, potentially influencing the disease process. Further studies are required to establish the exact role of miR-33-mediated Arc regulation in the development of epilepsy.http://www.sciencedirect.com/science/article/pii/S0168010224001093Temporal lobe epilepsyMiR-33ARC |
| spellingShingle | Yuchen Xu Lily Zhang Yan Yan Wenbiao Xiao Wei Zou Zhaohui Luo Bo Xiao Hongyu Long MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy Neuroscience Research Temporal lobe epilepsy MiR-33 ARC |
| title | MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy |
| title_full | MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy |
| title_fullStr | MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy |
| title_full_unstemmed | MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy |
| title_short | MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy |
| title_sort | microrna 33 regulates the synaptic plasticity related gene arc in temporal lobe epilepsy |
| topic | Temporal lobe epilepsy MiR-33 ARC |
| url | http://www.sciencedirect.com/science/article/pii/S0168010224001093 |
| work_keys_str_mv | AT yuchenxu microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy AT lilyzhang microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy AT yanyan microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy AT wenbiaoxiao microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy AT weizou microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy AT zhaohuiluo microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy AT boxiao microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy AT hongyulong microrna33regulatesthesynapticplasticityrelatedgenearcintemporallobeepilepsy |